FastQCFastQC Report
Fri 17 Jun 2016
SRR1526844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89589
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4330.48331826451908155No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3050.340443581243233No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2730.30472491042427086No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2490.2779359073100492No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2290.25561173804819787No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2250.2511469041958276No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2160.2411010280279945No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1940.21654444183995805No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.20203373181975465No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1570.17524472870553306No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1490.1663150610007925No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1410.15738539329605197No Hit
ACGAGCGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1320.14733951712821886No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC1260.14064226634966345No Hit
ACGAGCGGGATCACCTGCTGAGAAGAAAGTGCATTCTGTAGTGTTTGAGCAGAATGTTCTGTAG1200.13394501557110805No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1200.13394501557110805No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1190.1328288071080155No Hit
ACGAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1170.13059639018183036No Hit
ACGAGCGGGCAGCTTTCCTTTACACACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGA1160.1294801817187378No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1150.12836397325564522No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1100.1227829309401824No Hit
ACGAGCGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG1090.12166672247708982No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1080.12055051401399725No Hit
ACGAGCGGGGGAGCTACCCAGCTTCCTGTGGTACCAAGGAGTTATTTTGGATGATTAGAAGCAC1010.11273705477234928No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.11162084630925671No Hit
ACGAGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCACGCGA980.10938842938307158No Hit
ACGAGCGGGATTGGAGTTTTGGTTCTCTCCTGGTATCCACCTGGCATGGCTGATGATAATGGAG960.10715601245688644No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC950.10603980399379387No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT930.10380738706760875No Hit
ACGAGCGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC920.10269117860451618No Hit
ACGAGCGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC920.10269117860451618No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG910.10157497014142362No Hit
ACGAGCGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTACCGAACATCTAGA910.10157497014142362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACC201.9141762E-458.5178741
CTACGTA201.9141762E-458.5178739
CGTACTG201.9141762E-458.5178742
ACGTACT201.9141762E-458.5178741
TACGTAC201.9141762E-458.5178740
AGGGTTG150.00451132858.51786445
GAGTTAT150.00451132858.51786439
AGTTACC150.00451132858.51786440
GTCCATG150.00451132858.51786444
CATTTAA150.00451132858.51786441
TAATGTC150.00451132858.51786440
CTAATGT150.00451132858.51786439
CCATTTA150.00451132858.51786440
TAGCGCC201.9575472E-458.25353221
CCTAAAG201.9575472E-458.25353221
CTAGAAT150.00459298958.2535322
TAGAATG150.00459298958.2535323
ACACACC303.6114216E-758.2535322
AATAATT303.6114216E-758.2535322
TTCAATA150.00462389458.15501857
Produced by FastQC (version 0.11.5)