FastQCFastQC Report
Fri 17 Jun 2016
SRR1526843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81645
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4100.502174046175516No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3320.40663849592749096No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2880.3527466470696307No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2870.35152183232286116No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2450.30007961295854No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2430.2976299834650009No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2230.2731336885296099No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2200.26945924428930124No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2090.25598628207483615No Hit
ACGAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1920.23516443137975382No Hit
ACGAGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1720.2106681364443628No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1620.1984199889766673No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1530.1873966562557413No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1480.18127258252189354No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1430.1751485087880458No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1230.1506522138526548No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1220.14942739910588523No Hit
ACGAGCGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1120.13717925163818973No Hit
ACGAGCGGGTGATTGCACAAAAATAATTTTAAAAATGTGGTGTGAACATGTAAACA1080.1322799926511115No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1060.12983036315757243No Hit
ACGAGCGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1040.1273807336640333No Hit
ACGAGCGGGTGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTG1020.12493110417049422No Hit
ACGAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.12370628942372466No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.11635740094310734No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG950.11635740094310734No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG940.1151325861963378No Hit
ACGAGCGGGATTGGAGTTTTGGTTCTCTCCTGGTATCCACCTGGCATGGCTGATGA920.11268295670279871No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG920.11268295670279871No Hit
ACGAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG910.11145814195602916No Hit
ACGAGCGGGGGAGCTACCCAGCTTCCTGTGGTACCAAGGAGTTATTTTGGATGATT870.10655888296895095No Hit
ACGAGCGGGATGTTTTAATTTATGTTTTTAAAGCTATGGAAGGGGAGAATGAAGTG870.10655888296895095No Hit
ACGAGCGGGATCACCTGCTGAGAAGAAAGTGCATTCTGTAGTGTTTGAGCAGAATG860.1053340682221814No Hit
ACGAGCGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG850.10410925347541183No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG840.1028844387286423No Hit
ACGAGCGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTACCGAA820.1004348092351032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGATT150.00810210350.4631650
TTATAGA204.1487816E-450.0294741
TTTGGAT150.00838353550.0294746
AACTGTT150.00838353550.0294746
TTTATAG150.00838353550.0294740
GAGTTAT150.00838353550.0294739
TTAAAAC150.00838353550.0294744
CAACTGT150.00838353550.0294745
AACTAGC150.00838353550.0294746
GCGAGAA150.00840391249.99877533
GATGTTC150.00840391249.99877536
TTCAATC150.00840391249.99877535
GAAAGCG150.00840391249.99877533
ATTCAAT150.00840391249.99877534
TACTGAC150.00840391249.99877535
TAGGCGA150.00840391249.99877536
TCAAGCA150.00840391249.99877532
ATTGATG150.00840391249.99877533
TGATGTT150.00840391249.99877535
AAGAGAC204.1613774E-449.9987732
Produced by FastQC (version 0.11.5)