FastQCFastQC Report
Fri 17 Jun 2016
SRR1526841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60891
Sequences flagged as poor quality0
Sequence length64
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13032.139889310407121No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGTGGTATCAA4980.8178548553973493No Hit
CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.7291717987879982No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.5304560608300077No Hit
CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2020.3317403228720172No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.31203297695882803No Hit
CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.3038215828283326No Hit
CGTATTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.28739879456734163No Hit
CGTATTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.275902842784648No Hit
CGTATTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1590.26112233334975615No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.259480054523657No Hit
CGTATTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.25619549687145887No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.18721978617529683No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.18557750734919776No Hit
CGTATTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1090.17900839204480137No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT930.15273193082721584No Hit
CGTATTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.15273193082721584No Hit
CGTATTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG900.14780509434891856No Hit
CGTATTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.14123597904452218No Hit
CGTATTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAGATTAGC830.1363091425662249No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.13138230608792761No Hit
CGTATTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.11824407547913485No Hit
CGTATTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.11331723900083757No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGGGGTATCAA660.10839040252254029No Hit
CGTATTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.10017900839204481No Hit
CGTATTGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.10017900839204481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAC550.058.1469952
AGACACG550.058.1469955
AGCAGGG201.9703257E-458.14698851
CCTACCC150.004619340458.14698456
CGATACA150.004619340458.14698446
AACGATA150.004619340458.14698444
TGTCCTA150.004619340458.14698453
TCCTACC150.004619340458.14698455
ATGGCCT150.004619340458.14698450
CTAAGAC150.004619340458.14698450
TAAGACC150.004619340458.14698451
CTTAGGG150.004619340458.14698445
GAAGATT150.004619340458.14698455
GTCCTAC150.004619340458.14698454
CCTTCCA150.004619340458.14698455
CCTCAGG150.004619340458.14698454
TTGATCA303.6379242E-758.14698450
CCTTAGG150.004619340458.14698444
CCCCTTC150.004619340458.14698453
GATTAGC150.004619340458.14698458
Produced by FastQC (version 0.11.5)