FastQCFastQC Report
Fri 17 Jun 2016
SRR1526839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51873
Sequences flagged as poor quality0
Sequence length56
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT13712.642993464808282No Hit
CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5391.039076205347676No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGT3620.6978582306787731No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.6342413201472827No Hit
CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1910.36820696701559574No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGG1900.36627918184797487No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.36049582634511207No Hit
CGTATTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.32579569332793556No Hit
CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.3219401229926937No Hit
CGTATTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.32001233782507277No Hit
CGTATTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.3161567674898309No Hit
CGTATTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1230.23711757561737318No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.23518979044975227No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.1908507315944711No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG930.1792840205887456No Hit
CGTATTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.169645094750641No Hit
CGTATTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA870.16771730958302006No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.16193395408015732No Hit
CGTATTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC780.15036724307443178No Hit
CGTATTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.14651167273918994No Hit
CGTATTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1426561024039481No Hit
CGTATTGGGGAGAGTCACCCCTGGGAAAAGAACTCTAAAGATGAAATGTAAATTCC690.1330171765658435No Hit
CGTATTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGA650.12530603589535982No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.11952268039249705No Hit
CGTATTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.11566711005725522No Hit
CGTATTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.10602818421915061No Hit
CGTATTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.1041003990515297No Hit
CGTATTGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT520.10024482871628786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATTC150.0080789750.4731749
TTCGCGC150.0080789750.4731749
AAGATTA150.0080789750.4731748
TTGATCA251.9478139E-550.4731750
GATTAAG150.0080789750.4731750
GGTATTA150.0080789750.4731750
GTAAATT150.0080789750.4731748
GTCGGAG600.050.4731750
GTTCGCG150.0080789750.4731748
CGTTCGC150.00833145250.08228747
AACTGGG150.00833145250.08228740
CTCTCCT150.00833145250.08228740
CTAAGGT150.00833145250.08228740
GTTTCGG150.00833145250.08228745
ACTCTCC150.00833145250.08228739
AAGGTTT150.00833145250.08228742
TCGTTCG150.00833145250.08228746
AGGTTTC150.00833145250.08228743
AGACTCT150.00833145250.08228737
ATGTAAA150.00833145250.08228746
Produced by FastQC (version 0.11.5)