##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526839_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51873 Sequences flagged as poor quality 0 Sequence length 56 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.4771075511345 39.0 38.0 40.0 36.0 40.0 2 37.99518053708095 39.0 38.0 40.0 35.0 40.0 3 37.77618414203921 39.0 38.0 40.0 35.0 40.0 4 37.99681915447343 39.0 38.0 40.0 35.0 40.0 5 38.242052705646486 39.0 38.0 40.0 35.0 40.0 6 38.10205694677385 39.0 38.0 40.0 35.0 40.0 7 37.841613170628264 39.0 38.0 40.0 35.0 40.0 8 37.96304435833671 39.0 38.0 40.0 35.0 40.0 9 37.90906637364332 39.0 38.0 40.0 35.0 40.0 10 37.02319125556648 39.0 37.0 40.0 33.0 40.0 11 37.29047866905712 39.0 38.0 40.0 33.0 40.0 12 37.10192200181212 39.0 38.0 40.0 33.0 40.0 13 36.87342162589401 39.0 37.0 40.0 33.0 40.0 14 37.01748501147032 39.0 37.0 40.0 33.0 40.0 15 37.00015422281341 39.0 38.0 40.0 33.0 40.0 16 36.80635397991248 39.0 37.0 40.0 33.0 40.0 17 36.846663967767434 39.0 37.0 40.0 33.0 40.0 18 36.68052744202186 39.0 37.0 40.0 32.0 40.0 19 36.462764829487405 39.0 36.0 40.0 31.0 40.0 20 36.56806045534286 39.0 36.0 40.0 31.0 40.0 21 36.54891754862838 39.0 36.0 40.0 32.0 40.0 22 36.39033794073988 39.0 36.0 40.0 31.0 40.0 23 36.243074431785324 38.0 36.0 40.0 31.0 40.0 24 36.137952306594954 38.0 36.0 40.0 31.0 40.0 25 36.104524511788405 38.0 36.0 40.0 30.0 40.0 26 35.82117864785148 39.0 36.0 40.0 30.0 40.0 27 35.842152950475196 39.0 36.0 40.0 30.0 40.0 28 35.5695641277736 38.0 35.0 40.0 29.0 40.0 29 35.625084340601084 39.0 35.0 40.0 29.0 40.0 30 35.34100591830047 38.0 35.0 40.0 29.0 40.0 31 35.14163437626511 39.0 35.0 40.0 27.0 40.0 32 34.81988703178918 39.0 35.0 40.0 26.0 40.0 33 34.96227324426966 39.0 35.0 40.0 27.0 40.0 34 35.06353979912478 39.0 35.0 40.0 27.0 40.0 35 34.72743045515008 39.0 35.0 40.0 25.0 40.0 36 34.73115108052359 39.0 35.0 40.0 24.0 40.0 37 34.6082162203844 39.0 35.0 40.0 23.0 40.0 38 34.43047442792975 39.0 35.0 40.0 22.0 40.0 39 34.38000501224143 39.0 35.0 40.0 21.0 40.0 40 34.212056368438304 39.0 35.0 40.0 20.0 40.0 41 34.04805968422879 39.0 35.0 40.0 14.0 40.0 42 33.93568908680817 39.0 35.0 40.0 12.0 40.0 43 33.80874443352033 39.0 35.0 40.0 10.0 40.0 44 33.57149191294122 39.0 34.0 40.0 4.0 40.0 45 33.40452643957357 39.0 34.0 39.0 4.0 40.0 46 32.903051683920346 39.0 34.0 39.0 4.0 40.0 47 32.70499103579897 39.0 33.0 39.0 4.0 40.0 48 32.50266998245716 39.0 33.0 39.0 4.0 40.0 49 32.17317294160739 38.0 32.0 39.0 4.0 40.0 50 31.935785476066545 38.0 32.0 39.0 4.0 40.0 51 31.242129817053186 38.0 32.0 39.0 4.0 40.0 52 31.03763036647196 38.0 32.0 39.0 4.0 40.0 53 30.666319665336495 38.0 31.0 39.0 4.0 40.0 54 30.10909336263567 37.0 30.0 39.0 4.0 40.0 55 29.64708036936364 37.0 29.0 39.0 4.0 40.0 56 17.659437472288086 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.4649370442956169 1 2 -0.9624020766645103 1 3 -0.2963226756891473 1 4 -1.2964507886636412 1 5 -0.8755882849920482 1 6 -2.2996980937241176 1 7 -0.6926727109893918 1 8 -1.6426077283463627 1 9 -2.307947275168864 1 10 -0.7741063454319743 1 11 -1.1598565440579591 1 12 -1.4296562479354975 1 13 -1.9470997932318 1 14 -1.5613103834007518 1 15 -1.232851342240238 1 16 -0.4044986818189642 1 17 -1.060763915153359 1 18 -1.215511074434751 1 19 -0.9069291435286857 1 20 -0.6707535309705364 1 21 -0.03494471302133206 1 22 -0.7876394491941028 1 23 -0.557590244742471 1 24 -1.5685295231363057 1 25 -1.4249224831311338 1 26 -0.38253381282672194 1 27 -1.2482322053284562 1 28 -0.7664422279696268 1 29 -1.4977173192596709 1 30 -1.551932032323748 1 31 -1.666303192263662 1 32 -1.7252223659289783 1 33 -2.116061510284979 1 34 -2.1497983888169827 1 35 -1.50982688744309 1 36 -1.935667059705196 1 37 -1.5837863701341917 1 38 -1.0717082786373027 1 39 -1.9665607422529376 1 40 -1.970830644595985 1 41 -0.3258652170678218 1 42 -0.4012709234801193 1 43 -1.1133043953493456 1 44 -0.6331313126238669 1 45 -0.8603657811967764 1 46 -1.194633297416317 1 47 -0.42219602196772854 1 48 -0.7892518734778591 1 49 0.4768110766468041 1 50 0.5852305186558233 1 51 1.566387546215104 1 52 0.5665633507847581 1 53 -0.08284316337856268 1 54 -0.2135899594383197 1 55 0.561310738490608 1 56 0.34459732270105903 2 1 -0.5215408178805205 2 2 -0.8114586804380934 2 3 -0.8623604115382051 2 4 -0.27758286413533995 2 5 -0.23407885102978554 2 6 -0.37516979183732246 2 7 -0.20210667325354592 2 8 -0.37845678495013857 2 9 -0.553230294036787 2 10 -1.321276156752731 2 11 -1.235328242171164 2 12 -1.0900336064260614 2 13 -1.5886092271940626 2 14 -1.410366987174335 2 15 -0.6290777573345778 2 16 -0.6497817006868871 2 17 -0.8343488208137373 2 18 -1.0645676782083342 2 19 -0.850325369943782 2 20 -1.387734663046011 2 21 -1.6575862224552935 2 22 -1.466884732212968 2 23 -1.387778923987753 2 24 -0.45532197596649127 2 25 -1.2173753133198133 2 26 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2.0682743386679405 3 52 2.935997313048908 3 53 1.384326647942192 3 54 2.61961758773149 3 55 2.3937635686792866 3 56 0.6857293981727572 4 1 0.06336484249683849 4 2 -0.05674169930601636 4 3 -0.23971890210424363 4 4 -0.05116776979571824 4 5 -0.06426753027506749 4 6 0.03992454778531851 4 7 0.005440496557774566 4 8 0.20644887542722046 4 9 0.20148668709528295 4 10 -0.1892006850546153 4 11 -0.1221206950013567 4 12 0.1363814879135603 4 13 0.0906360558248025 4 14 0.17453867320302408 4 15 0.2954505445522173 4 16 0.18040697855839483 4 17 0.33546249994098076 4 18 0.029771944433178987 4 19 0.28175010175433357 4 20 0.27264269544456 4 21 0.4744892492428221 4 22 0.4387756451455189 4 23 0.7442965477103556 4 24 0.6390176466750148 4 25 0.29205864894434086 4 26 0.3344473192487456 4 27 0.5630885493885245 4 28 0.6863879607096237 4 29 0.5400185297969315 4 30 0.7310868356007774 4 31 0.8997345435853958 4 32 0.6710040491653615 4 33 0.09148565952634158 4 34 1.0200129319377353 4 35 1.1128146219908714 4 36 1.3662197327476306 4 37 1.472817403450712 4 38 1.6830087024947744 4 39 0.8070241634074407 4 40 1.1612448271021307 4 41 0.315644216894448 4 42 0.372313982180259 4 43 -0.03783269723613358 4 44 -0.2746407465861296 4 45 -0.2565921962911162 4 46 -0.779538957793676 4 47 -0.7995545125337671 4 48 -0.034534892345782 4 49 0.137188435137368 4 50 0.4531550469577077 4 51 0.056953583950956954 4 52 0.2269407092753255 4 53 -0.32812618224648915 4 54 -0.5909484500043582 4 55 -0.4952930350942957 4 56 -0.5044592810725241 5 1 0.13883654061004336 5 2 0.037597923335489725 5 3 0.062167890348582944 5 4 0.13751147548730103 5 5 0.10554379047965057 5 6 0.32294341570984386 5 7 0.6092140814634348 5 8 0.5649394414649578 5 9 0.5788451776613215 5 10 0.16928988098311493 5 11 -0.4428754119824845 5 12 -0.35418454982229264 5 13 0.14723982940971325 5 14 -0.1273481192498025 5 15 -0.478134361108161 5 16 -0.9894043421963232 5 17 -0.2871790094929807 5 18 -1.1589073008498403 5 19 -0.5484385774909484 5 20 -0.3688667385177098 5 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1.2134297013123785 8 15 0.6936022580678127 8 16 0.754916027460979 8 17 0.21416869940190253 8 18 1.0270765266434196 8 19 0.28829611638660424 8 20 -0.2248544166078119 8 21 0.5857714979913737 8 22 0.8346170005556104 8 23 0.6060601210642247 8 24 1.195043830733546 8 25 0.6836643478276656 8 26 1.3544704228297988 8 27 1.8480327157343126 8 28 1.8350209988305295 8 29 1.4345121763121398 8 30 2.339866196401701 8 31 2.860458455791786 8 32 2.2824788277560444 8 33 2.6659947084289257 8 34 2.8471211337090097 8 35 2.629564718255793 8 36 0.964217422389531 8 37 1.1713156706821337 8 38 1.4284842512048215 8 39 0.984151618162926 8 40 0.823162424329702 8 41 0.8709002815844684 8 42 1.3091641939630847 8 43 0.6548896747315212 8 44 1.6025252141377848 8 45 1.8092530097158175 8 46 2.121500703353803 8 47 2.5932063654023167 8 48 1.7529121619476342 8 49 2.5214780000199255 8 50 1.7165358709854353 8 51 2.766233522341409 8 52 2.7290585375387053 8 53 2.9133062397789224 8 54 3.5118624856906777 8 55 2.5782533645976535 8 56 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-1.7078840368079469 120 41 -1.3479447032179621 120 42 -1.790271324925321 120 43 -1.837511541073951 120 44 -2.1369450420497955 120 45 -1.8025576720036796 120 46 -0.838551402594959 120 47 -1.7385348417188347 120 48 -1.4520379031848023 120 49 -2.5384438410569317 120 50 -4.113685579296806 120 51 -4.174278852821423 120 52 -3.413364389078751 120 53 -4.386737181845042 120 54 -3.1216590080131894 120 55 -4.199427920682414 120 56 -1.9721429422527734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 147.0 5 13.0 6 86.0 7 54.0 8 324.0 9 265.0 10 68.0 11 82.0 12 92.0 13 98.0 14 158.0 15 170.0 16 186.0 17 212.0 18 253.0 19 314.0 20 274.0 21 348.0 22 356.0 23 332.0 24 419.0 25 502.0 26 543.0 27 497.0 28 637.0 29 792.0 30 961.0 31 1275.0 32 1664.0 33 1881.0 34 2449.0 35 3233.0 36 4099.0 37 7753.0 38 11459.0 39 9877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.50070364158618 0.3026622713164845 0.12145046556011799 0.07518362153721589 8 99.14961723133882 0.5592086233826338 0.1369096974488517 0.15426444782969204 9 95.392593449386 2.642993464808282 0.9176257397875581 1.0467873460181596 10 26.51861276579338 47.329053650261216 9.823993214196209 16.328340369749196 11 21.903495074508896 41.84064927804446 19.41472442311029 16.84113122433636 12 46.1665991941858 20.210899697337727 18.566498949357083 15.056002159119389 13 24.68143350105064 18.105758294295686 27.30900468451796 29.903803520135714 14 26.181250361459718 31.900988953790986 22.882809939660323 19.034950745088967 15 36.548878992925026 22.566653172170493 23.711757561737322 17.17271027316716 16 22.828831954966937 24.831800744125072 29.109556031075893 23.22981126983209 17 30.740462282883197 25.126751874771074 19.308696238891137 24.824089603454592 18 29.423785013398106 27.66950051086307 21.88614500800031 21.020569467738515 19 19.45906348196557 27.30322132901509 29.965492645499587 23.27222254351975 20 20.1071869216533 34.17257865515114 20.08212523133868 25.638109191856877 21 21.891928363503173 24.558055250322905 29.124978312416864 24.42503807375706 22 22.753648333429723 25.926782719333758 22.196518419987278 29.123050527249244 23 25.560503537485786 33.566595338615464 22.99462147938234 17.878279644516414 24 20.157310302282543 36.83490129549661 25.13109191856878 17.876696483652065 25 22.974718938619667 23.88684266347841 27.60283086179301 25.535607536108916 26 26.293452679501524 23.63131293645588 29.74458891160924 20.33064547243335 27 17.982946872950343 23.698830973417184 30.819090242679113 27.499131910953356 28 17.82778185186615 31.670462001621058 33.73731135898723 16.76444478752557 29 23.81917892866798 23.26690611362144 35.548217665005986 17.365697292704592 30 15.47961885618199 31.9236939252788 38.500937397321174 14.095749821218037 31 20.312560442536075 25.888747050404238 41.28080151638234 12.517890990677344 32 12.0032488252016 24.281100732919494 51.58090154899344 12.13474889288546 33 15.77359074554107 24.792045498510465 44.72279181336326 14.711571942585215 34 13.823592005726779 26.252249114864473 46.731286396966354 13.192872482442395 35 10.899235311199302 32.1827509437615 47.2771270932146 9.640886651824605 36 13.380336251646394 25.439683892461456 48.07662508716201 13.103354768730146 37 11.068709683668132 24.876508533018228 53.19140693101912 10.86337485229452 38 12.62135922330097 25.77950894327849 52.04348584384629 9.555645989574248 39 10.906271202869052 24.93942037413977 50.99738296016284 13.156925462828342 40 10.71012805587893 24.565386107877377 54.695382227396195 10.029103608847498 41 9.806889859291607 26.307617661329452 51.22367782629791 12.66181465308103 42 12.994021274943707 24.409892072365867 51.283096513704486 11.312990138985946 43 12.471355886122655 24.478580028741213 53.44311958674797 9.606944498388161 44 10.38895469639008 27.151096180360025 52.46907586849719 9.990873254752703 45 10.906936539694257 26.910899166682857 52.80783201569511 9.37433227792778 46 10.235531890085118 26.26608107582883 52.54965214349566 10.948734890590385 47 12.256446155641115 27.447205693618017 51.12588962781472 9.170458522926147 48 14.551679863043013 27.066513627609286 49.395949652744 8.9858568566037 49 12.651188114961304 28.347528487535488 49.605258040679814 9.396025356823397 50 10.995640666355285 29.80694379573408 50.159582749494 9.037832788416628 51 10.000583691655155 30.167130377259372 50.31811195205946 9.514173979026014 52 12.619223731848795 28.763576906606453 48.32210845953206 10.29509090201269 53 11.24435446192182 28.92656907023828 48.253776670300574 11.575299797539323 54 11.705298662587756 30.181982193983607 48.209593285484566 9.90312585794407 55 12.48661175050145 29.91957313392144 47.08963798173356 10.504177133843548 56 12.857504429258416 29.5524015341783 47.175982711290224 10.414111325273057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.5 4 9.0 5 13.0 6 16.0 7 19.0 8 135.0 9 251.0 10 240.0 11 229.0 12 224.0 13 266.0 14 313.0 15 611.5 16 910.0 17 1077.0 18 1244.0 19 1378.5 20 1513.0 21 1854.5 22 2569.0 23 2942.0 24 3190.5 25 3439.0 26 3800.0 27 4161.0 28 4164.0 29 3902.5 30 3638.0 31 2917.0 32 2196.0 33 2426.5 34 2657.0 35 1822.5 36 988.0 37 905.0 38 833.0 39 844.0 40 878.0 41 912.0 42 955.5 43 999.0 44 1075.5 45 1152.0 46 1144.0 47 1148.5 48 1161.0 49 1257.5 50 1354.0 51 1775.5 52 2197.0 53 2153.0 54 1835.0 55 1561.0 56 1518.0 57 1475.0 58 1457.0 59 1439.0 60 1316.0 61 1193.0 62 981.0 63 660.5 64 552.0 65 466.0 66 380.0 67 320.5 68 261.0 69 220.5 70 180.0 71 151.5 72 90.0 73 57.0 74 45.5 75 34.0 76 31.0 77 28.0 78 21.5 79 13.0 80 11.0 81 7.5 82 4.0 83 4.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.026988992346692883 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0019277851676209204 21 0.0 22 0.0 23 0.0 24 0.0019277851676209204 25 0.030844562681934726 26 0.06747248086673222 27 0.06747248086673222 28 0.10602818421915061 29 0.16771730958302006 30 0.2583232124612033 31 0.3296512636631774 32 0.3123011971545891 33 0.3450735450041447 34 0.3585680411774912 35 0.42025716654136064 36 0.47230736606712553 37 0.48194629190523003 38 0.5205019952576485 39 0.5552021282748251 40 0.6419524608177665 41 0.6708692383320802 42 0.6882193048406686 43 0.7306305785283288 44 0.724847223025466 45 0.7556917857074008 46 0.8000308445626819 47 0.8617199699265514 48 0.9060590287818325 49 0.8617199699265514 50 0.94268694696663 51 0.9176257397875581 52 0.9619647986428392 53 0.9735315096485647 54 1.6945231623387889 55 1.0063038574981205 56 0.9831704354866694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 51873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.461338268463365 #Duplication Level Percentage of deduplicated Percentage of total 1 73.84249242752055 39.477184662541205 2 13.237415260349056 14.153798700672798 3 5.304341554882446 8.507315944711122 4 2.57103706908986 5.498043298054864 5 1.4820424058848984 3.961598519460991 6 0.955574787249387 3.0651784165172633 7 0.6634934371844801 2.4829872958957453 8 0.40386557046011823 1.7272955101883445 9 0.3425645463724218 1.648256318315887 >10 1.0962065483917496 9.500125306035894 >50 0.05408913890090869 2.1128525437125285 >100 0.039665368527333045 4.183293813737397 >500 0.0036059425933939134 1.039076205347676 >1k 0.0036059425933939134 2.642993464808282 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1371 2.642993464808282 No Hit CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 539 1.039076205347676 No Hit CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGT 362 0.6978582306787731 No Hit CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.6342413201472827 No Hit CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 191 0.36820696701559574 No Hit CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGG 190 0.36627918184797487 No Hit CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.36049582634511207 No Hit CGTATTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169 0.32579569332793556 No Hit CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.3219401229926937 No Hit CGTATTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 166 0.32001233782507277 No Hit CGTATTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 164 0.3161567674898309 No Hit CGTATTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 123 0.23711757561737318 No Hit CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.23518979044975227 No Hit CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.1908507315944711 No Hit CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 93 0.1792840205887456 No Hit CGTATTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.169645094750641 No Hit CGTATTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 87 0.16771730958302006 No Hit CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.16193395408015732 No Hit CGTATTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 78 0.15036724307443178 No Hit CGTATTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.14651167273918994 No Hit CGTATTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 0.1426561024039481 No Hit CGTATTGGGGAGAGTCACCCCTGGGAAAAGAACTCTAAAGATGAAATGTAAATTCC 69 0.1330171765658435 No Hit CGTATTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGA 65 0.12530603589535982 No Hit CGTATTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.11952268039249705 No Hit CGTATTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.11566711005725522 No Hit CGTATTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.10602818421915061 No Hit CGTATTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.1041003990515297 No Hit CGTATTGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 52 0.10024482871628786 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.003855570335241841 0.0 0.0 0.0 0.0 8 0.0057833555028627606 0.0 0.0 0.0 0.0 9 0.009638925838104601 0.0 0.0 0.0 0.0 10 0.05205019952576485 0.0 0.0 0.0 0.0 11 0.07903919187245774 0.0 0.0 0.0 0.0 12 0.07903919187245774 0.0 0.0 0.0 0.0 13 0.08675033254294141 0.0 0.0 0.0 0.0 14 0.1041003990515297 0.0 0.0 0.0 0.0 15 0.1137393248896343 0.0 0.0 0.0 0.0 16 0.1233782507277389 0.0 0.0 0.0 0.0 17 0.1310893913982226 0.0 0.0 0.0 0.0 18 0.1330171765658435 0.0 0.0 0.0 0.0 19 0.15036724307443178 0.0 0.0 0.0 0.0 20 0.18313959092398743 0.0 0.0 0.0 0.0 21 0.19856187226495478 0.0 0.0 0.0 0.0 22 0.21205636843830122 0.0 0.0 0.0 0.0 23 0.23326200528213137 0.0 0.0 0.0 0.0 24 0.2563954272935824 0.0 0.0 0.0 0.0 25 0.26025099762882425 0.0 0.0 0.0 0.0 26 0.27760106413741253 0.0 0.0 0.0 0.0 27 0.28723998997551714 0.0 0.0 0.0 0.0 28 0.3894126038594259 0.0 0.0 0.0 0.0 29 0.3990515296975305 0.0 0.0 0.0 0.0 30 0.4144738110384979 0.0 0.0 0.0 0.0 31 0.42025716654136064 0.0 0.0 0.0 0.0 32 0.4279683072118443 0.0 0.0 0.0 0.0 33 0.4376072330499489 0.0 0.0 0.0 0.0 34 0.45110172922329533 0.0 0.0 0.0 0.0 35 0.5012241435814393 0.0 0.0 0.0 0.0 36 0.5166464249224066 0.0 0.0 0.0 0.0 37 0.5282131359281321 0.0 0.0 0.0 0.0 38 0.5359242765986159 0.0 0.0 0.0 0.0 39 0.557129913442446 0.0 0.0 0.0 0.0 40 0.5686966244481715 0.0 0.0 0.0 0.0 41 0.5783355502862761 0.0 0.0 0.0 0.0 42 0.5860466909567598 0.0 0.0 0.0 0.0 43 0.6014689722977271 0.0 0.0 0.0 0.0 44 0.6226746091415573 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATTC 15 0.00807897 50.47317 49 TTCGCGC 15 0.00807897 50.47317 49 AAGATTA 15 0.00807897 50.47317 48 TTGATCA 25 1.9478139E-5 50.47317 50 GATTAAG 15 0.00807897 50.47317 50 GGTATTA 15 0.00807897 50.47317 50 GTAAATT 15 0.00807897 50.47317 48 GTCGGAG 60 0.0 50.47317 50 GTTCGCG 15 0.00807897 50.47317 48 CGTTCGC 15 0.008331452 50.082287 47 AACTGGG 15 0.008331452 50.082287 40 CTCTCCT 15 0.008331452 50.082287 40 CTAAGGT 15 0.008331452 50.082287 40 GTTTCGG 15 0.008331452 50.082287 45 ACTCTCC 15 0.008331452 50.082287 39 AAGGTTT 15 0.008331452 50.082287 42 TCGTTCG 15 0.008331452 50.082287 46 AGGTTTC 15 0.008331452 50.082287 43 AGACTCT 15 0.008331452 50.082287 37 ATGTAAA 15 0.008331452 50.082287 46 >>END_MODULE