FastQCFastQC Report
Fri 17 Jun 2016
SRR1526838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9343
Sequences flagged as poor quality0
Sequence length64
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1371.4663384351921225No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.8990688215776517No Hit
GAACGCGGGATGTGGACCCGAGTAGATCCATAATCATGGAAGCAGCACGTGGCACCTGCATCTT420.44953441078882583No Hit
GAACGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.438831210531949No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG390.4174248100181954No Hit
GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.3746120089906882No Hit
GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA290.3103928074494274No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.27828320667879697No Hit
GAACGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2568768061650433No Hit
GAACGCGGGTACTTTTTAAAGTTAAGTTTATGTTCTTCCAGAACCTAGTGTCTTAAAGTTGCCT200.2140640051375361No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1926576046237825No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.18195440436690571No Hit
GAACGCGGGCCTTGTATTCGTTTGTGAGTATTTACGGATTATGTACTCTTCTGTTCCTCGTGCA170.18195440436690571No Hit
GAACGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAA160.1712512041100289No Hit
GAACGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.14984480359627528No Hit
GAACGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.13914160333939848No Hit
GAACGCGGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCTGATAATGT130.13914160333939848No Hit
GAACGCGGGATGGCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.12843840308252166No Hit
GAACGCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.12843840308252166No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12843840308252166No Hit
GAACGCGGGAGCTGTTGTAGAATTCCGTAGTTCCCCATGCAGCCCGTTGCTCTCCTGGCTTCTC120.12843840308252166No Hit
GAACGCGGGATAGCAAGACCCCATCTCAAAAGAAAAGAAAAAGACCCAAAATAGTTTCAGTCCT120.12843840308252166No Hit
GAACGCGGGGTGCCTACCTTCTCACTCCTGTTGTCCTGCCTCGGTTTGCCCAATTGTCCTCATC110.11773520282564486No Hit
GAACGCGGGAAGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10703200256876805No Hit
GAACGCGGGAGAGTTTCAAGCAGAGTCGACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10703200256876805No Hit
GAACGCGGGCAGTACGCAGAGTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10703200256876805No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10703200256876805No Hit
GAACGCGGGAACAGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10703200256876805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGACA201.8184852E-458.55978858
GGAAGAC150.004371634758.55978457
CATCCCT150.00446725258.24324816
CATCTCA150.00446725258.24324811
ATGGTCA150.00446725258.24324811
CCATCAA150.00446725258.24324816
GCCATCA150.00446725258.24324815
TGTGGCC150.00446725258.24324811
TCATCCC150.00446725258.24324815
TCTCATC150.00446725258.24324813
ATCCCTG150.00446725258.24324817
GGACCCG257.815619E-658.24324414
TGGACCC257.815619E-658.24324413
TGTGGAC257.815619E-658.24324411
GACCCGA257.815619E-658.24324415
GTGGACC257.815619E-658.24324412
ATGAGAT257.815619E-658.24324411
GTAGATC258.072931E-657.9301122
GCGGGAC501.8189894E-1257.930115
GGATGAG258.072931E-657.930119
Produced by FastQC (version 0.11.5)