##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526838_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9343 Sequences flagged as poor quality 0 Sequence length 64 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.50219415605266 38.0 38.0 39.0 35.0 40.0 2 36.55763673338328 38.0 35.0 39.0 31.0 39.0 3 37.56095472546291 39.0 38.0 39.0 35.0 40.0 4 36.96425131114203 38.0 36.0 39.0 33.0 40.0 5 36.740126297763034 38.0 35.0 39.0 33.0 39.0 6 36.62774269506583 38.0 35.0 39.0 33.0 39.0 7 37.249598629990366 38.0 37.0 39.0 34.0 40.0 8 36.846195012308684 38.0 36.0 39.0 33.0 40.0 9 36.97484747939634 38.0 36.0 39.0 33.0 40.0 10 34.95301295087231 37.0 33.0 39.0 27.0 39.0 11 35.38338863320133 38.0 35.0 39.0 29.0 39.0 12 35.53655142887723 38.0 35.0 39.0 29.0 39.0 13 35.465803275179276 38.0 35.0 39.0 29.0 39.0 14 35.30429198330301 38.0 34.0 39.0 29.0 39.0 15 35.3920582254094 38.0 35.0 39.0 29.0 39.0 16 35.13678689928289 38.0 33.0 39.0 28.0 39.0 17 35.155196403724716 38.0 33.0 39.0 29.0 39.0 18 34.93149951835599 37.0 33.0 39.0 27.0 39.0 19 34.70309322487424 37.0 33.0 38.0 27.0 39.0 20 34.782189874772556 37.0 33.0 38.0 27.0 39.0 21 34.764208498341006 37.0 33.0 38.0 27.0 39.0 22 34.335973456063364 36.0 33.0 38.0 27.0 39.0 23 34.17296371615113 36.0 33.0 38.0 27.0 39.0 24 33.89393128545435 36.0 32.0 38.0 26.0 39.0 25 33.983303007599275 36.0 33.0 38.0 25.0 39.0 26 34.253237718077706 36.0 33.0 39.0 27.0 39.0 27 34.14663384351921 36.0 33.0 39.0 26.0 39.0 28 33.97623889542973 36.0 33.0 38.0 25.0 39.0 29 33.86203574868886 36.0 33.0 38.0 25.0 39.0 30 33.57679546184309 36.0 32.0 38.0 23.0 39.0 31 33.48795889971101 36.0 32.0 39.0 23.0 39.0 32 32.9523707588569 36.0 31.0 39.0 22.0 39.0 33 33.27314567055549 36.0 32.0 38.0 23.0 39.0 34 32.98233971957615 35.0 31.0 39.0 23.0 39.0 35 32.61885903885262 35.0 31.0 38.0 21.0 39.0 36 32.48753077170074 35.0 31.0 39.0 18.0 39.0 37 32.138499411323984 35.0 31.0 38.0 18.0 39.0 38 31.782189874772556 35.0 30.0 38.0 17.0 39.0 39 31.590602590174463 35.0 29.0 38.0 17.0 39.0 40 31.353205608476934 35.0 29.0 38.0 15.0 39.0 41 31.368618216846837 35.0 29.0 38.0 13.0 39.0 42 30.970780263298725 35.0 29.0 38.0 11.0 39.0 43 30.729530129508724 35.0 29.0 38.0 11.0 39.0 44 30.2035748688858 35.0 27.0 38.0 10.0 39.0 45 30.117521138820507 35.0 27.0 38.0 10.0 39.0 46 29.465910307181847 35.0 26.0 38.0 4.0 39.0 47 28.935887830461308 34.0 24.0 38.0 4.0 39.0 48 28.929679974312318 34.0 25.0 37.0 4.0 39.0 49 28.493952691854865 33.0 23.0 37.0 4.0 39.0 50 28.0849834100396 33.0 23.0 37.0 4.0 39.0 51 27.689714224553143 33.0 22.0 37.0 4.0 39.0 52 27.224446109386708 32.0 19.0 37.0 4.0 39.0 53 26.81419244354062 32.0 17.0 37.0 4.0 39.0 54 26.279460558707054 32.0 16.0 36.0 4.0 39.0 55 25.792786043026865 32.0 11.0 36.0 4.0 39.0 56 24.971850583324414 32.0 8.0 36.0 4.0 39.0 57 24.007492240179815 30.0 4.0 35.0 4.0 39.0 58 23.55089371722145 30.0 4.0 35.0 4.0 39.0 59 22.83463555603125 29.0 4.0 35.0 4.0 39.0 60 22.228941453494595 29.0 4.0 35.0 4.0 39.0 61 21.565557101573372 28.0 4.0 35.0 4.0 39.0 62 20.982232687573585 27.0 4.0 35.0 4.0 38.0 63 20.52542010061008 25.0 4.0 35.0 4.0 38.0 64 20.3752542010061 25.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4481859410430928 1 2 2.3760637588368567 1 3 1.4876950780312086 1 4 1.933506736027752 1 5 0.40287114845938277 1 6 1.250250100040013 1 7 1.1726223822862494 1 8 -1.2565826330532275 1 9 1.2702480992397014 1 10 -0.2300753634787256 1 11 1.963061891423223 1 12 3.373202614379089 1 13 1.1751433906896054 1 14 -1.8441476590636157 1 15 -0.11238161931438384 1 16 2.2555455515539506 1 17 -4.110734293717478 1 18 -0.9321795384820604 1 19 3.0727524343070627 1 20 -0.9229325063358758 1 21 1.226137121515265 1 22 2.402007469654528 1 23 3.691593303988263 1 24 3.8412264905962346 1 25 1.5256269174336339 1 26 2.019321061758035 1 27 2.8097572362278314 1 28 3.7858043217286905 1 29 1.8996765372815645 1 30 -0.019171001734036963 1 31 1.6853307989862714 1 32 0.9579365079365161 1 33 4.762058156595977 1 34 4.086087768440713 1 35 0.8634420434840706 1 36 0.04385420835000531 1 37 2.2173369347739182 1 38 3.641356542617043 1 39 4.810214085634247 1 40 1.3535414165666353 1 41 4.655578898225947 1 42 2.5157396291850134 1 43 3.9463618780845664 1 44 2.433133253301328 1 45 -1.1116146458583387 1 46 1.1197378951580568 1 47 0.11494264372415586 1 48 -8.754528478057892 1 49 0.0021541950113359576 1 50 -0.799269707883159 1 51 2.9147458983593424 1 52 3.0624283046551852 1 53 2.6580532212885117 1 54 2.485697612378292 1 55 2.603971588635467 1 56 3.5866913432039453 1 57 1.401704014939309 1 58 3.1285580899026293 1 59 5.288992263572101 1 60 9.7219154328398 1 61 4.961978124583169 1 62 9.74729225023344 1 63 6.11396225156728 1 64 4.706029078297984 2 1 0.5910430839002387 2 2 0.8939209016940026 2 3 1.2376950780312086 2 4 1.451363878884898 2 5 1.9028711484593828 2 6 1.0359643857542977 2 7 0.7619080965719647 2 8 1.0469887955182031 2 9 -0.4976090436174445 2 10 0.6627817793784203 2 11 1.7309190342803689 2 12 0.15891690009337367 2 13 0.4965719621181748 2 14 0.012995198079238435 2 15 -0.8623816193143838 2 16 3.3626884086968047 2 17 1.174979991996807 2 18 2.8356776043750855 2 19 1.537038148592778 2 20 0.48778177937840894 2 21 1.5475656929438344 2 22 1.3484360410830973 2 23 0.7451647325596937 2 24 2.68051220488195 2 25 -1.0279445111377967 2 26 1.4836067760437501 2 27 3.5776143790849773 2 28 -1.5356242496998789 2 29 4.11396225156728 2 30 -0.30488528744832166 2 31 2.4174736561291255 2 32 1.7436507936508008 2 33 2.0477724423102615 2 34 4.711087768440713 2 35 5.077727757769786 2 36 2.347425636921436 2 37 1.4673369347739182 2 38 2.998499399759897 2 39 1.881642657062816 2 40 5.710684273709489 2 41 3.548436041083093 2 42 -0.26997465652927133 2 43 4.857076163798851 2 44 2.6474189675870434 2 45 3.0133853541416613 2 46 4.423309323729484 2 47 7.007799786581298 2 48 0.5133286647992499 2 49 4.841439909297051 2 50 5.200730292116841 2 51 7.504031612645058 2 52 4.919571161798043 2 53 3.800910364145654 2 54 6.682126183806865 2 55 7.211114445778325 2 56 8.05097705748966 2 57 10.383846872082167 2 58 12.432129518474056 2 59 9.413992263572101 2 60 8.882629718554085 2 61 9.551263838868884 2 62 7.193720821662012 2 63 11.685390822995853 2 64 12.616743364012269 3 1 0.3688208616780173 3 2 1.5189209016940026 3 3 0.3765839669200943 3 4 1.631919434440455 3 5 0.513982259570497 3 6 1.5359643857542977 3 7 -0.7797585700946996 3 8 -2.1613445378151326 3 9 -0.5392757102841088 3 10 0.801670668267306 3 11 0.8836968120581474 3 12 -0.8966386554621835 3 13 2.5243497398959533 3 14 -0.7647825796985401 3 15 -0.0012705082032695714 3 16 -0.9984227024143095 3 17 -2.158353341336529 3 18 -0.23376684006935733 3 19 0.12037148192611369 3 20 -0.6372182206215911 3 21 0.019787915166055825 3 22 0.30676937441643304 3 23 -1.213168600773642 3 24 0.5555122048819499 3 25 0.5414999333066461 3 26 1.8447178871548644 3 27 -0.8251633986928013 3 28 -2.8967353608109896 3 29 -4.38603774843272 3 30 1.584003601440564 3 31 0.4313625450180112 3 32 -1.86746031746031 3 33 -2.730005335467517 3 34 -3.2472455648926193 3 35 -3.6444944644524355 3 36 -0.6386854741896748 3 37 0.550670268107254 3 38 -3.05705615579566 3 39 -3.618357342937184 3 40 -0.9004268374016213 3 41 -3.8682306255835748 3 42 0.4522475656929501 3 43 -2.656812725090038 3 44 -0.18591436574628872 3 45 2.1106075763638827 3 46 -1.3405795651594055 3 47 0.7022442310257411 3 48 -2.056115779645193 3 49 -1.3946712018140595 3 50 3.0896191810057303 3 51 -0.14874616513272088 3 52 -0.41376217153528927 3 53 2.0231325863678755 3 54 4.834903961584644 3 55 3.016670001333882 3 56 0.8565326130452178 3 57 1.481069094304388 3 58 4.8904628518073885 3 59 1.7334367080165443 3 60 2.118740829665196 3 61 5.231819394424441 3 62 0.98538748832868 3 63 2.018724156329185 3 64 0.7695211417900474 4 1 0.03548752834468161 4 2 0.7411431239162241 4 3 0.1543617446978729 4 4 0.6319194344404551 4 5 1.6250933706816042 4 6 -0.5751467253568165 4 7 1.109130318794186 4 8 0.5053221288515388 4 9 0.016279845271441218 4 10 3.0238928904895346 4 11 -0.7829698546085169 4 12 0.9922502334267094 4 13 0.3021275176737319 4 14 0.9018840869681242 4 15 2.887618380685616 4 16 2.5571328531412476 4 17 -0.15835334133652879 4 18 2.2106776043750855 4 19 1.4537048152594423 4 20 2.473892890489523 4 21 -0.42465652927838704 4 22 -1.4710084033613455 4 23 -1.213168600773642 4 24 0.22217887154861415 4 25 1.2081665999733175 4 26 -0.821948779511807 4 27 -2.269607843137244 4 28 0.5477090836334568 4 29 1.8361844737895012 4 30 0.8062258236627926 4 31 0.0980292116846826 4 32 3.1325396825396865 4 33 1.3811057756435972 4 34 2.752754435107377 4 35 1.9110610911031216 4 36 2.58353674803255 4 37 0.7728924903294754 4 38 2.054054955315454 4 39 1.7149759903961517 4 40 0.4329064959317108 4 41 1.5762138188608716 4 42 2.785580899026286 4 43 0.009853941576629666 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7 18 0.21067760437508554 7 19 -4.837961851407222 7 20 1.237781779378409 7 21 -0.7024343070561656 7 22 1.2234360410830973 7 23 -1.3798352674403063 7 24 3.68051220488195 7 25 -2.0279445111377967 7 26 -2.76639322395625 7 27 -0.6723856209150227 7 28 -2.035624249699879 7 29 -2.01103774843272 7 30 -0.9298852874483217 7 31 0.04247365612912546 7 32 0.9936507936508008 7 33 -2.5772275576897385 7 34 0.5860877684407129 7 35 -0.2972722422302141 7 36 0.2224256369214359 7 37 0.5923369347739182 7 38 1.623499399759897 7 39 -0.993357342937184 7 40 3.3356842737094894 7 41 3.298436041083093 7 42 1.8550253434707287 7 43 -1.517923836201149 7 44 -1.2275810324129566 7 45 1.7633853541416613 7 46 0.04830932372948382 7 47 3.132799786581298 7 48 1.5133286647992499 7 49 2.3414399092970513 7 50 -0.924269707883159 7 51 -0.6209683873549423 7 52 -4.455428838201957 7 53 -8.199089635854346 7 54 -5.692873816193135 7 55 -3.4138855542216753 7 56 -2.0740229425103394 7 57 -3.6161531279178334 7 58 -1.3178704815259437 7 59 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118 58 3.8904628518073885 118 59 6.06677004134988 118 60 1.0076297185540852 118 61 0.8984860610911056 118 62 4.318720821662012 118 63 0.4631686007736313 118 64 1.7695211417900474 119 1 -1.1589569160997613 119 2 -2.7310790983059974 119 3 -3.1373049219687914 119 4 -0.42363612111510207 119 5 -1.7221288515406172 119 6 0.4109643857542977 119 7 -1.7380919034280353 119 8 -2.828011204481797 119 9 -1.9976090436174445 119 10 -3.9622182206215797 119 11 0.48091903428036886 119 12 1.0339169000933737 119 13 -0.12842803788182522 119 14 -1.8620048019207616 119 15 -2.987381619314384 119 16 -1.0123115913031953 119 17 -0.07502000800319308 119 18 1.3356776043750855 119 19 1.537038148592778 119 20 1.112781779378409 119 21 1.1725656929438344 119 22 -1.1515639589169027 119 23 -4.629835267440306 119 24 0.43051220488194986 119 25 0.3470554888622033 119 26 -1.3913932239562499 119 27 0.32761437908497726 119 28 -0.16062424969987887 119 29 0.9889622515672798 119 30 -2.6798852874483217 119 31 1.1674736561291255 119 32 3.618650793650801 119 33 -0.07722755768973855 119 34 -3.413912231559287 119 35 -0.6722722422302141 119 36 -1.777574363078564 119 37 -6.407663065226082 119 38 -6.001500600240103 119 39 -3.868357342937184 119 40 -2.2893157262905106 119 41 -3.326563958916907 119 42 -1.2699746565292713 119 43 -5.017923836201149 119 44 -5.352581032412957 119 45 -3.2366146458583387 119 46 -3.326690676270516 119 47 -3.617200213418702 119 48 -4.73667133520075 119 49 -2.5335600907029487 119 50 -0.799269707883159 119 51 -5.245968387354942 119 52 -4.205428838201957 119 53 -5.574089635854346 119 54 -7.567873816193135 119 55 -7.288885554221675 119 56 -10.57402294251034 119 57 -9.366153127917833 119 58 -8.317870481525944 119 59 -7.336007736427899 119 60 -10.367370281445915 119 61 -9.448736161131116 119 62 -9.306279178337988 119 63 -7.939609177004147 119 64 -8.383256635987731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 0.0 6 4.0 7 3.0 8 5.0 9 2.0 10 3.0 11 7.0 12 12.0 13 23.0 14 32.0 15 37.0 16 48.0 17 63.0 18 94.0 19 102.0 20 128.0 21 149.0 22 146.0 23 180.0 24 194.0 25 275.0 26 261.0 27 337.0 28 408.0 29 426.0 30 581.0 31 625.0 32 700.0 33 740.0 34 753.0 35 883.0 36 763.0 37 743.0 38 452.0 39 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.38139136027442 1.500696752063458 0.032157787544216956 0.08575410011791189 8 99.30212583208073 0.6012454369765944 0.021473051320592657 0.0751556796220743 9 96.88536872524885 1.8837632452103177 0.6528952156694852 0.5779728138713475 10 33.57593920582254 44.00085625602055 8.551857005244567 13.87134753291234 11 22.58375254201006 39.559028149416676 20.66787969602911 17.18933961254415 12 39.92293695815049 17.1679332120304 23.943058974633416 18.966070855185702 13 25.55924221342181 17.232152413571658 31.296157551107783 25.91244782189875 14 23.70758856898213 30.62185593492454 25.66627421599058 20.004281280102752 15 34.29305362303329 21.962966927111204 24.884940597238575 18.859038852616933 16 22.851332548431984 27.34667665632024 27.164722251953332 22.637268543294446 17 27.25032147449636 24.603514787826832 23.071153021860265 25.075010715816543 18 27.121909450925823 29.990367119768813 20.646473295515356 22.241250133790004 19 20.81772449962539 28.020978272503477 29.198330300759928 21.962966927111204 20 20.753505298084125 29.037782296906776 22.0592957294231 28.149416675585996 21 22.337578936101895 26.972064647329553 26.93995504655892 23.750401370009634 22 22.530236540725674 28.128010275072246 25.238146205715513 24.103606978486567 23 26.98276784758643 31.16771914802526 23.579150165899605 18.27036283848871 24 22.134218131221235 36.7333832816012 22.6586749438082 18.473723643369368 25 21.99507652788184 25.74119661778872 25.27025580648614 26.993471047843304 26 23.779443254817988 28.329764453961452 26.895074946466806 20.995717344753746 27 19.728109612502674 24.94112609719546 29.394134018411478 25.936630271890387 28 16.154587303286586 30.788994754308963 32.66245583984584 20.393962102558614 29 20.520231213872833 24.470134874759154 34.06122885891672 20.948405052451296 30 14.830281614733911 32.50883392226148 37.52007709604882 15.140807366955777 31 16.557780871800365 26.61454428617329 41.276641319481634 15.551033522544714 32 12.37146529562982 26.745929734361614 45.64053127677806 15.242073693230507 33 17.0932847809789 25.575666702366927 42.19770804326872 15.133340473385456 34 14.69737546866631 28.323513658275306 43.985002678093196 12.994108194965184 35 11.037291041577369 32.46892413201886 43.67766823831976 12.81611658808401 36 14.036215579127825 26.850958962820098 43.2229722490089 15.889853209043181 37 14.564355374557925 25.28132033008252 49.24445397063552 10.90987032472404 38 13.043944265809218 27.395498392282956 47.09539121114684 12.465166130760986 39 11.94253859348199 27.144082332761577 47.85591766723842 13.057461406518009 40 10.816895368782161 27.75514579759863 47.90951972555746 13.51843910806175 41 13.005253564919053 26.128444301490294 46.703119974268255 14.163182159322396 42 13.242547716062619 26.635213381942958 45.732361140896415 14.389877761098004 43 13.288288288288289 26.351351351351347 47.77992277992278 12.58043758043758 44 11.432861432861433 26.99485199485199 48.12312312312312 13.449163449163448 45 16.08751608751609 25.557700557700556 44.755469755469754 13.5993135993136 46 13.087320317528429 25.13409139669599 46.620896803261104 15.157691482514482 47 13.826021666845437 27.27662769494798 47.17365654832136 11.72369408988523 48 16.032171581769436 27.378016085790886 44.9544235924933 11.63538873994638 49 13.542225560682478 27.76048932288872 45.07994420002146 13.61734091640734 50 12.371244635193133 26.90987124463519 46.866952789699575 13.851931330472103 51 12.427559562137798 26.636617299849753 46.41553981541103 14.520283322601415 52 14.8840206185567 27.01890034364261 45.45747422680412 12.639604810996563 53 13.684662444993023 26.61801008908447 44.552967693463565 15.144359772458946 54 13.570968434614558 28.258535537899938 44.40627013098562 13.764225896499893 55 15.11053874221936 28.139085640695427 42.14423696072119 14.606138656364026 56 15.554839402728543 28.144806101622088 41.85197121065635 14.44838328499302 57 16.378662659654395 28.281635719652247 42.363421702264674 12.976279918428679 58 16.582590962756253 29.451540195341845 39.916282065042395 14.049586776859504 59 12.913267496779735 31.483469300128807 39.62000858737655 15.983254615714898 60 14.649339490924712 31.317796155085382 39.716464396949846 14.31639995704006 61 13.850118101782263 29.750912604681123 41.357096843461456 15.041872450075155 62 13.76246207195492 30.830082358040745 37.75465973125271 17.652795838751626 63 15.60855086475454 31.077451928241484 37.85583843592222 15.458158771081749 64 15.13588999892577 31.303040068750672 38.44666451820819 15.114405414115373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 2.0 9 1.0 10 12.0 11 24.0 12 38.0 13 77.5 14 103.0 15 83.5 16 96.5 17 129.0 18 137.5 19 146.0 20 190.5 21 267.5 22 300.0 23 335.0 24 405.0 25 440.0 26 489.0 27 515.5 28 493.0 29 517.5 30 454.5 31 367.0 32 383.5 33 400.0 34 337.5 35 226.5 36 178.0 37 168.5 38 181.5 39 204.0 40 177.0 41 155.5 42 161.0 43 154.0 44 147.0 45 162.5 46 186.0 47 194.0 48 190.5 49 176.0 50 165.0 51 204.0 52 271.0 53 299.0 54 318.0 55 330.0 56 323.0 57 335.5 58 348.0 59 305.5 60 251.0 61 239.0 62 225.5 63 211.0 64 210.0 65 180.5 66 145.0 67 139.0 68 117.0 69 95.0 70 76.0 71 58.5 72 60.0 73 55.5 74 43.5 75 36.0 76 32.5 77 20.5 78 12.0 79 14.0 80 12.0 81 8.0 82 6.5 83 5.0 84 3.5 85 2.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.14984480359627528 8 0.3103928074494274 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.11773520282564486 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.032109600770630416 27 0.010703200256876806 28 0.02140640051375361 29 0.010703200256876806 30 0.04281280102750722 31 0.06421920154126083 32 0.07492240179813764 33 0.06421920154126083 34 0.08562560205501445 35 0.11773520282564486 36 0.10703200256876805 37 0.12843840308252166 38 0.13914160333939848 39 0.1605480038531521 40 0.1605480038531521 41 0.1712512041100289 42 0.18195440436690571 43 0.2033608048806593 44 0.2033608048806593 45 0.2033608048806593 46 0.22476720539441292 47 0.2140640051375361 48 0.1926576046237825 49 0.2568768061650433 50 0.24617360590816653 51 0.26758000642192015 52 0.33179920796318096 53 0.27828320667879697 54 0.3103928074494274 55 0.26758000642192015 56 0.36390880873381143 57 0.27828320667879697 58 0.27828320667879697 59 0.28898640693567373 60 0.3425024082200578 61 0.3103928074494274 62 1.2308680295408327 63 0.36390880873381143 64 0.36390880873381143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 9343.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.46216418709194 #Duplication Level Percentage of deduplicated Percentage of total 1 87.53773178094006 65.18248956437975 2 6.338939197930142 9.440222626565344 3 2.443582003737243 5.458632131007171 4 1.5667672847491734 4.666595311998288 5 0.7187005893344833 2.6758000642192017 6 0.4887164007474486 2.1834528524028682 7 0.301854247520483 1.5733704377608904 8 0.11499209429351731 0.6850048164401156 9 0.086244070720138 0.5779728138713475 >10 0.3737243064539313 5.1910521245852514 >50 0.014374011786689664 0.8990688215776517 >100 0.014374011786689664 1.4663384351921225 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 1.4663384351921225 No Hit GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.8990688215776517 No Hit GAACGCGGGATGTGGACCCGAGTAGATCCATAATCATGGAAGCAGCACGTGGCACCTGCATCTT 42 0.44953441078882583 No Hit GAACGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.438831210531949 No Hit GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG 39 0.4174248100181954 No Hit GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.3746120089906882 No Hit GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA 29 0.3103928074494274 No Hit GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.27828320667879697 No Hit GAACGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.2568768061650433 No Hit GAACGCGGGTACTTTTTAAAGTTAAGTTTATGTTCTTCCAGAACCTAGTGTCTTAAAGTTGCCT 20 0.2140640051375361 No Hit GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.1926576046237825 No Hit GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.18195440436690571 No Hit GAACGCGGGCCTTGTATTCGTTTGTGAGTATTTACGGATTATGTACTCTTCTGTTCCTCGTGCA 17 0.18195440436690571 No Hit GAACGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAA 16 0.1712512041100289 No Hit GAACGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.14984480359627528 No Hit GAACGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.13914160333939848 No Hit GAACGCGGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCTGATAATGT 13 0.13914160333939848 No Hit GAACGCGGGATGGCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.12843840308252166 No Hit GAACGCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.12843840308252166 No Hit GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.12843840308252166 No Hit GAACGCGGGAGCTGTTGTAGAATTCCGTAGTTCCCCATGCAGCCCGTTGCTCTCCTGGCTTCTC 12 0.12843840308252166 No Hit GAACGCGGGATAGCAAGACCCCATCTCAAAAGAAAAGAAAAAGACCCAAAATAGTTTCAGTCCT 12 0.12843840308252166 No Hit GAACGCGGGGTGCCTACCTTCTCACTCCTGTTGTCCTGCCTCGGTTTGCCCAATTGTCCTCATC 11 0.11773520282564486 No Hit GAACGCGGGAAGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10703200256876805 No Hit GAACGCGGGAGAGTTTCAAGCAGAGTCGACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10703200256876805 No Hit GAACGCGGGCAGTACGCAGAGTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10703200256876805 No Hit GAACGCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.10703200256876805 No Hit GAACGCGGGAACAGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10703200256876805 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.010703200256876806 0.0 0.0 0.0 0.0 8 0.010703200256876806 0.0 0.0 0.0 0.0 9 0.010703200256876806 0.0 0.0 0.0 0.0 10 0.010703200256876806 0.0 0.0 0.0 0.0 11 0.02140640051375361 0.0 0.0 0.0 0.0 12 0.02140640051375361 0.0 0.0 0.0 0.0 13 0.02140640051375361 0.0 0.0 0.0 0.0 14 0.032109600770630416 0.0 0.0 0.0 0.0 15 0.04281280102750722 0.0 0.0 0.0 0.0 16 0.04281280102750722 0.0 0.0 0.0 0.0 17 0.04281280102750722 0.0 0.0 0.0 0.0 18 0.04281280102750722 0.0 0.0 0.0 0.0 19 0.04281280102750722 0.0 0.0 0.0 0.0 20 0.04281280102750722 0.0 0.0 0.0 0.0 21 0.04281280102750722 0.0 0.0 0.0 0.0 22 0.06421920154126083 0.0 0.0 0.0 0.0 23 0.06421920154126083 0.0 0.0 0.0 0.0 24 0.06421920154126083 0.0 0.0 0.0 0.0 25 0.07492240179813764 0.0 0.0 0.0 0.0 26 0.07492240179813764 0.0 0.0 0.0 0.0 27 0.08562560205501445 0.0 0.0 0.0 0.0 28 0.11773520282564487 0.0 0.0 0.0 0.0 29 0.11773520282564487 0.0 0.0 0.0 0.0 30 0.11773520282564487 0.0 0.0 0.0 0.0 31 0.12843840308252166 0.0 0.0 0.0 0.0 32 0.13914160333939848 0.0 0.0 0.0 0.0 33 0.13914160333939848 0.0 0.0 0.0 0.0 34 0.14984480359627528 0.0 0.0 0.0 0.0 35 0.1712512041100289 0.0 0.0 0.0 0.0 36 0.1712512041100289 0.0 0.0 0.0 0.0 37 0.1926576046237825 0.0 0.0 0.0 0.0 38 0.1926576046237825 0.0 0.0 0.0 0.0 39 0.1926576046237825 0.0 0.0 0.0 0.0 40 0.22476720539441292 0.0 0.0 0.0 0.0 41 0.23547040565128974 0.0 0.0 0.0 0.0 42 0.24617360590816653 0.0 0.0 0.0 0.0 43 0.26758000642192015 0.0 0.0 0.0 0.0 44 0.27828320667879697 0.0 0.0 0.0 0.0 45 0.27828320667879697 0.0 0.0 0.0 0.0 46 0.27828320667879697 0.0 0.0 0.0 0.0 47 0.27828320667879697 0.0 0.0 0.0 0.0 48 0.27828320667879697 0.0 0.0 0.0 0.0 49 0.2889864069356738 0.0 0.0 0.0 0.0 50 0.2889864069356738 0.0 0.0 0.0 0.0 51 0.2889864069356738 0.0 0.0 0.0 0.0 52 0.2889864069356738 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGACA 20 1.8184852E-4 58.559788 58 GGAAGAC 15 0.0043716347 58.559784 57 CATCCCT 15 0.004467252 58.243248 16 CATCTCA 15 0.004467252 58.243248 11 ATGGTCA 15 0.004467252 58.243248 11 CCATCAA 15 0.004467252 58.243248 16 GCCATCA 15 0.004467252 58.243248 15 TGTGGCC 15 0.004467252 58.243248 11 TCATCCC 15 0.004467252 58.243248 15 TCTCATC 15 0.004467252 58.243248 13 ATCCCTG 15 0.004467252 58.243248 17 GGACCCG 25 7.815619E-6 58.243244 14 TGGACCC 25 7.815619E-6 58.243244 13 TGTGGAC 25 7.815619E-6 58.243244 11 GACCCGA 25 7.815619E-6 58.243244 15 GTGGACC 25 7.815619E-6 58.243244 12 ATGAGAT 25 7.815619E-6 58.243244 11 GTAGATC 25 8.072931E-6 57.93011 22 GCGGGAC 50 1.8189894E-12 57.93011 5 GGATGAG 25 8.072931E-6 57.93011 9 >>END_MODULE