FastQCFastQC Report
Fri 17 Jun 2016
SRR1526837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11961
Sequences flagged as poor quality0
Sequence length64
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA2462.056684223727113No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2452.048323718752613No Hit
GAACGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAA1551.2958782710475714No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1401.1704706964300644No Hit
GAACGCGGGGGTCCCTTCCCTCCAGGGCTATCCCATCTGCTGCCCTACTTCTACGTCCTCTACT1190.9949000919655547No Hit
GAACGCGGGAACCATTACCAGATGAAAAACCCAAATGGCCCGTACCCATACACTCTGAAGTTGT1010.8444110024245465No Hit
GAACGCGGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCTGATAATGT860.7190034278070395No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG790.6604798929855363No Hit
GAACGCGGGATGTGGACCCGAGTAGATCCATAATCATGGAAGCAGCACGTGGCACCTGCATCTT500.41802524872502295No Hit
GAACGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.401304238776022No Hit
GAACGCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGA450.3762227238525207No Hit
GAACGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.2675361591840147No Hit
GAACGCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAG310.2591756542095142No Hit
GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.2591756542095142No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.2508151492350138No Hit
GAACGCGGGCCTTGTATTCGTTTGTGAGTATTTACGGATTATGTACTCTTCTGTTCCTCGTGCA300.2508151492350138No Hit
GAACGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG250.20901262436251147No Hit
GAACGCGGGTACTTTTTAAAGTTAAGTTTATGTTCTTCCAGAACCTAGTGTCTTAAAGTTGCCT250.20901262436251147No Hit
GAACGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG220.18393110943901012No Hit
GAACGCGGGAGCTGTTGTAGAATTCCGTAGTTCCCCATGCAGCCCGTTGCTCTCCTGGCTTCTC220.18393110943901012No Hit
GAACGCGGGCACTGAGCTACATTGCCAGCCTCAAGAGCTTCAGGATCGACATCCAGAGAATGGA210.17557060446450964No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGACTCTGGAAACAGACAAACCT210.17557060446450964No Hit
GAACGCGGGAGTTTGGAAGCAGCAGTGAATTCAGAGAAGGTTCCTGTAAAGTCCATTTCTCTGT210.17557060446450964No Hit
GAACGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG200.1672100994900092No Hit
GAACGCGGGTACTACAGCCTCGTGCTGAAGTGTTATCACTAAACTGTCACCTTTGCTCCTATTC190.15884959451550873No Hit
GAACGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.15884959451550873No Hit
GAACGCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.15048908954100826No Hit
GAACGCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATG180.15048908954100826No Hit
GAACGCGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCTCAAAACCC170.14212858456650782No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14212858456650782No Hit
GAACGCGGGGGGTGGGGCCCAGTCCGAACCTGGCCCTCCCGCCCCCATCTGCCTTTAGGTCGCT170.14212858456650782No Hit
GAACGCGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCAT170.14212858456650782No Hit
GAACGCGGGATCCAGAAGGTAATTTTTAAAGTTCTGAGGGCCTCCCTTTCTGCTTCAGGAGAAA160.13376807959200734No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.13376807959200734No Hit
GAACGCGGGAGCGAACCCGGGGAACTGAAACATCTAAGTACCCGGAGGAAAGGACATCAAACGA160.13376807959200734No Hit
GAACGCAGGACTTAAAGGCTCTTAATACCACCTACCAATCCCAGCTCATCAAACCCAGCCGCAT160.13376807959200734No Hit
GAACGCGGGACTATGAGGCTCTGAGCCTCTCTGTGCCTTGGGCCCTAAAGCCCCATGTGTAGTA150.1254075746175069No Hit
GAACGCGGGGTGCCTACCTTCTCACTCCTGTTGTCCTGCCTCGGTTTGCCCAATTGTCCTCATC150.1254075746175069No Hit
GAACGCGGGAGTTACTTCTCCATCTCAACTAAGGTGTAAAGTGGGTTTGCCCCACTACTGCAAG150.1254075746175069No Hit
GAACGCGGGGGCCATCTTGCGTCCCCGCGTGTGTGCGCCTTCTCTCAGCTGGTCTGCCCGAGAC150.1254075746175069No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA150.1254075746175069No Hit
GAACGCGGGACTGCACAATAGCTCCTCCCAGGGTTTTAACTTTGTTTTATTTTCAAAACCAGGT140.11704706964300643No Hit
GAACGCGGGATCATTGGGTCACCTCAAGTTTGCCTGTGTACAGCTCTGCTTGGCCATGAGCCGC140.11704706964300643No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11704706964300643No Hit
GAACGCGGGATGTGGGATGTTATTTGTCCTGGGCAGATTTCCATATCTGATGGTTGTCAGCGCT140.11704706964300643No Hit
GAACGCGGGAAGCAGTGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11704706964300643No Hit
GAACGCGGGGGGATGAGTCTCCGGGTGGGTCAGGAGCAAACCTCCCTGACCAGTGTTCAATGCT130.10868656466850597No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC130.10868656466850597No Hit
GAACGCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10868656466850597No Hit
GAACGCGGGATGGCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1003260596940055No Hit
GAACGCGGGGTTGCGAAAGAGCCCCCTGCTCCAAGTTTTCGTCCATCAGAGCAGCATATTCCTC120.1003260596940055No Hit
GAACGCGGGGTCTTTGGTTCGTCCTCAGTGCAGGGCAACAGGACTTTGGGCTCAGGATGGTGGC120.1003260596940055No Hit
GAACGCGGGAAAGAAATTCTGTGGTACTTGTTTGTTTTGTTACTGTTTTTATTCAGGTTTTGGT120.1003260596940055No Hit
GAACGCGGGTGATGTAGGGCTCTGGATGGTGGCACCGCCTGGAAGAGGCGTGAGGCGTGTTGAA120.1003260596940055No Hit
GAACGCGGGCAGTACGCAGAGTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1003260596940055No Hit
GAACGCGGGCAGCTACTTTTCCACAGGCTGTCTCTGAAAAGTTCCTGTAGATATTACAGGGATG120.1003260596940055No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1003260596940055No Hit
GAACGCGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCAACATGCC120.1003260596940055No Hit
GAACGCGGGGGTTTCCTCTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1003260596940055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT150.004495612358.25316643
GTTTGAT150.004495612358.25316648
TGATCAT150.004495612358.25316651
ACGTAGA150.004495612358.25316641
TCATGGC150.004495612358.25316654
TTGATCA150.004495612358.25316650
TAGAGTT150.004495612358.25316644
ATCATGG150.004495612358.25316653
CATGGCT150.004495612358.25316655
AGTTTGA150.004495612358.25316647
TTTGATC150.004495612358.25316649
GTGTCTC150.00457166758.00840425
AAACTGG201.928881E-458.00840439
GGGCAAA201.928881E-458.00840435
TGCGTGT150.00457166758.00840422
TTCCGGG201.928881E-458.00840431
TGTCTCC150.00457166758.00840426
CAGTACG150.00457166758.00840437
GGCAAAC201.928881E-458.00840436
GCGTGTC150.00457166758.00840423
Produced by FastQC (version 0.11.5)