##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526837_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11961 Sequences flagged as poor quality 0 Sequence length 64 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.400802608477555 39.0 38.0 40.0 35.0 40.0 2 37.92634395117465 39.0 38.0 40.0 35.0 40.0 3 38.39269291865229 39.0 38.0 40.0 36.0 40.0 4 38.15985285511245 39.0 38.0 40.0 35.0 40.0 5 37.968648106345626 39.0 38.0 40.0 35.0 40.0 6 37.91129504222055 39.0 38.0 40.0 35.0 40.0 7 38.50464008026085 39.0 38.0 40.0 36.0 40.0 8 38.44929353732965 39.0 38.0 40.0 36.0 40.0 9 38.46425884123401 39.0 38.0 40.0 36.0 40.0 10 37.5088203327481 39.0 38.0 40.0 33.0 40.0 11 37.492182927848845 39.0 38.0 40.0 33.0 40.0 12 37.62411169634646 39.0 38.0 40.0 33.0 40.0 13 37.557562076749434 39.0 38.0 40.0 33.0 40.0 14 37.466934202825854 39.0 37.0 40.0 33.0 40.0 15 37.57536995234512 39.0 38.0 40.0 33.0 40.0 16 37.25624947746844 39.0 37.0 40.0 33.0 40.0 17 37.24638408159853 39.0 37.0 40.0 33.0 40.0 18 37.182844243792324 39.0 36.0 40.0 33.0 40.0 19 37.02549954017223 39.0 36.0 40.0 33.0 40.0 20 37.06144971156258 39.0 36.0 40.0 33.0 40.0 21 37.04046484407658 39.0 36.0 40.0 33.0 40.0 22 36.91773263105092 39.0 36.0 40.0 32.0 40.0 23 36.74174400133768 38.0 36.0 40.0 31.0 40.0 24 36.61817573781456 38.0 36.0 40.0 31.0 40.0 25 36.71616085611571 38.0 36.0 40.0 31.0 40.0 26 37.303151910375384 39.0 38.0 40.0 33.0 40.0 27 37.221971407072985 39.0 37.0 40.0 33.0 40.0 28 37.10091129504222 39.0 37.0 40.0 33.0 40.0 29 37.15575620767494 39.0 37.0 40.0 33.0 40.0 30 37.034779700693925 39.0 37.0 40.0 33.0 40.0 31 37.10275060613661 39.0 37.0 40.0 33.0 40.0 32 36.92826686731878 39.0 36.0 40.0 33.0 40.0 33 36.978513502215534 39.0 37.0 40.0 33.0 40.0 34 36.90945573112616 39.0 37.0 40.0 32.0 40.0 35 36.6518685728618 39.0 36.0 40.0 32.0 40.0 36 36.475043892651115 39.0 36.0 40.0 31.0 40.0 37 36.370788395619094 39.0 36.0 40.0 31.0 40.0 38 36.250313518936544 39.0 36.0 40.0 31.0 40.0 39 36.215199398043644 39.0 36.0 40.0 31.0 40.0 40 35.937630632890226 39.0 35.0 40.0 30.0 40.0 41 35.59635481983112 39.0 35.0 40.0 29.0 40.0 42 35.533818242621855 39.0 35.0 40.0 29.0 40.0 43 35.34913468773514 39.0 35.0 40.0 28.0 40.0 44 35.18426552963799 39.0 35.0 40.0 28.0 40.0 45 35.039043558230915 39.0 35.0 40.0 27.0 40.0 46 34.44946074742914 39.0 35.0 40.0 24.0 40.0 47 34.23074993729621 39.0 34.0 39.0 23.0 40.0 48 34.298302817490175 39.0 35.0 40.0 23.0 40.0 49 33.99264275562244 38.0 34.0 39.0 21.0 40.0 50 33.78889724939386 38.0 34.0 39.0 19.0 40.0 51 33.22439595351559 38.0 33.0 39.0 8.0 40.0 52 33.119806036284594 38.0 33.0 39.0 7.0 40.0 53 32.73095894992058 38.0 33.0 39.0 4.0 40.0 54 32.33617590502466 37.0 32.0 39.0 4.0 40.0 55 31.964635063957864 37.0 32.0 39.0 4.0 40.0 56 31.335005434328234 37.0 31.0 39.0 4.0 40.0 57 31.037204247136525 36.0 31.0 39.0 4.0 40.0 58 30.68388930691414 36.0 30.0 39.0 4.0 40.0 59 30.12064208678204 36.0 29.0 39.0 4.0 40.0 60 29.876515341526627 36.0 29.0 39.0 4.0 40.0 61 29.45823927765237 36.0 28.0 39.0 4.0 40.0 62 29.18376389933952 35.0 27.0 39.0 4.0 40.0 63 28.90025917565421 35.0 26.0 39.0 4.0 40.0 64 28.595602374383414 35.0 23.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5121486823167345 1 2 -1.7978724123681857 1 3 -1.057217828856487 1 4 -2.7397011485246807 1 5 -1.822552424233109 1 6 -2.278509142584774 1 7 -3.9684810987331858 1 8 -2.1424116266553526 1 9 -2.634195403102993 1 10 -2.629157303947224 1 11 -3.457538563210832 1 12 -2.06471336786462 1 13 -0.6018926451699613 1 14 -2.6569684517163665 1 15 -0.9089195584993846 1 16 -0.3281249096375163 1 17 -0.9875619012173544 1 18 -1.723234701806149 1 19 -0.5188898869570977 1 20 -0.37096332705576174 1 21 -0.0976826394893564 1 22 -1.3194139194139396 1 23 -0.025284939150488128 1 24 -0.329946990451198 1 25 -0.3427562866638567 1 26 -1.519141642671066 1 27 -0.07811635143567486 1 28 -2.3021619370358692 1 29 -2.6359904334694377 1 30 -1.7668360817520465 1 31 -1.171619533594317 1 32 -2.3392935262683068 1 33 -3.2723825194413365 1 34 -1.6938142623016716 1 35 -1.228873787277152 1 36 -4.946740794219785 1 37 -6.251867180438612 1 38 -5.623125427327121 1 39 -2.885796229913886 1 40 -4.530881723318686 1 41 -5.145072201374713 1 42 -5.756348670004126 1 43 -4.959569725326023 1 44 -4.170192482587449 1 45 -4.082067768832481 1 46 -3.2838435840536597 1 47 -4.360681452068011 1 48 -3.7410050500386696 1 49 -4.66176053171851 1 50 -3.1587101740463055 1 51 -3.4465334152308955 1 52 -2.806337639951078 1 53 -2.88697150188748 1 54 -2.0345420219369856 1 55 -3.527484816770521 1 56 -3.3974720424300244 1 57 -2.9598168638084594 1 58 -1.6539477096199882 1 59 -1.2511893801809677 1 60 -4.011642209331281 1 61 0.8750936271944596 1 62 -1.5522205618844325 1 63 -1.9136039264190607 1 64 -2.115393360141251 2 1 -0.4212395914076481 2 2 -0.5251451396409124 2 3 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1.7388984265034608 2 45 0.645204958440246 2 46 -0.9202072204172964 2 47 -2.451590542977101 2 48 -1.741005050038666 2 49 -0.2981241680821469 2 50 -1.976891992228122 2 51 -4.719260687958165 2 52 -5.169974003587441 2 53 -3.88697150188748 2 54 -3.307269294664259 2 55 -2.891121180406884 2 56 -5.579290224248208 2 57 -5.232544136535733 2 58 -7.381220436892715 2 59 -6.978462107453694 2 60 -6.920733118422191 2 61 -6.852179100078267 2 62 -6.097675107338979 2 63 -6.186331199146334 2 64 -6.479029723777614 3 1 1.1242149540468986 3 2 0.6566730421772675 3 3 0.6700548984162396 3 4 1.2602988514753193 3 5 1.2683566666759774 3 6 1.4487635846879598 3 7 0.6678825376304474 3 8 0.8575883733446474 3 9 0.8203500514424675 3 10 1.6435699687800494 3 11 0.9970068913346211 3 12 1.2989229957717399 3 13 0.1253800821027653 3 14 1.25212245737454 3 15 0.3638077142278888 3 16 0.21732963581703046 3 17 0.37607446241900533 3 18 -0.45050742907887553 3 19 0.11747374940654254 3 20 1.0835821274896915 3 21 1.175044633237917 3 22 1.0442224442224202 3 23 1.1565332426676918 3 24 0.3973257368215286 3 25 0.9299709860634096 3 26 1.0263129027834808 3 27 -1.2599345332538547 3 28 0.6978380629641308 3 29 1.0003732028942025 3 30 0.6877093727934067 3 31 0.8283804664056831 3 32 -0.0665662535410334 3 33 0.5457992987404836 3 34 1.4880039195165082 3 35 -2.3197828781862384 3 36 -0.5831044305834183 3 37 -0.7064126349840691 3 38 -0.07767088187257798 3 39 0.023294679177027433 3 40 0.19639100395404085 3 41 0.6731096168071034 3 42 0.6981967845413308 3 43 1.22224845649216 3 44 1.1025347901398206 3 45 1.0997504129856992 3 46 1.4434291432190705 3 47 1.1847730933865321 3 48 1.6226313135976937 3 49 0.7927849228269466 3 50 -2.6132556285917588 3 51 -1.9010788697763488 3 52 -1.624519458132898 3 53 -2.3415169564329332 3 54 -1.8527238401188058 3 55 -1.2547575440432475 3 56 -0.6701993151572978 3 57 -0.6870895910811896 3 58 0.43696138128910533 3 59 -3.9784621074536943 3 60 -2.2843694820585547 3 61 -2.2158154637146303 3 62 -3.915856925520796 3 63 -5.368149380964514 3 64 -5.660847905595798 4 1 0.7605785904105389 4 2 0.20212758763181427 4 3 0.3973276256889662 4 4 0.5330261242025855 4 5 1.2683566666759774 4 6 0.9033090392334131 4 7 0.576973446721361 4 8 0.1303156460719137 4 9 0.45671368780610067 4 10 1.0981154233255026 4 11 1.360643254970988 4 12 1.0261957230444665 4 13 0.2162891730118588 4 14 1.25212245737454 4 15 1.181989532409709 4 16 1.6718750903624837 4 17 1.8306199169644657 4 18 1.3676743891029446 4 19 2.208382840315629 4 20 2.174491218398785 4 21 0.9932264514197371 4 22 1.589676989676967 4 23 1.7019877881222385 4 24 1.670053009548802 4 25 1.1117891678815965 4 26 1.3899492664198405 4 27 1.1037018303825121 4 28 1.0614744266004976 4 29 3.7320289420250674E-4 4 30 1.14225482733886 4 31 -1.353437715412504 4 32 -2.7029298899046665 4 33 -1.5451097921686099 4 34 -1.6938142623016716 4 35 -2.3197828781862384 4 36 -0.9467407942197852 4 37 -0.6155035440749757 4 38 -0.7140345182362111 4 39 -0.24943259355024594 4 40 -1.5308817233186858 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118 4 -4.703337512161049 118 5 -4.313461515142201 118 6 -5.11487277894841 118 7 -4.0139356441877325 118 8 -4.406047990291718 118 9 -4.243286312193902 118 10 -5.829157303947223 118 11 -5.257538563210829 118 12 -5.410167913319164 118 13 -6.610983554260869 118 14 -4.875150269898185 118 15 -5.236192285772113 118 16 -4.53721581872842 118 17 -4.360289173944629 118 18 -3.5777801563516007 118 19 -4.673435341502554 118 20 -4.316417872510307 118 21 -5.497682639489355 118 22 -4.764868464868485 118 23 -4.452557666423218 118 24 -5.884492444996653 118 25 -4.606392650300222 118 26 -5.04641436994379 118 27 -4.559934533253852 118 28 -5.129434664308597 118 29 -3.590535888014891 118 30 -5.257745172661139 118 31 -3.489801351776137 118 32 -3.29383898081376 118 33 -3.6178370648958804 118 34 -3.293814262301673 118 35 -5.110691969095331 118 36 -2.5467407942197866 118 37 -5.4882308168022504 118 38 -4.704943609145303 118 39 -4.394887139004794 118 40 -6.630881723318687 118 41 -4.46325401955653 118 42 -6.147257760913217 118 43 -6.377751543507841 118 44 -5.333828846223813 118 45 -4.036613223377934 118 46 -3.320207220417295 118 47 -6.924317815704374 118 48 -5.731914140947758 118 49 -5.179942349900326 118 50 -4.858710174046305 118 51 -1.2010788697763495 118 52 -2.3154285490419895 118 53 -3.3415169564329332 118 54 -0.14363293102789498 118 55 -2.3365757258614295 118 56 0.5570734121154288 118 57 -0.3052714092630069 118 58 0.6915068358345593 118 59 1.794265165273579 118 60 1.7429032452141726 118 61 3.4841845362853725 118 62 2.384143074479205 118 63 4.0591233463082155 118 64 4.011879367131474 119 1 -1.3303305004985546 119 2 -2.1387815032772792 119 3 -0.7466117682504247 119 4 -1.1033375121610476 119 5 -1.0801281818088668 119 6 -0.664872778948407 119 7 -1.2639356441877325 119 8 -0.33938132362505513 119 9 -0.37661964552723504 119 10 -1.879157303947224 119 11 -0.3742052298774965 119 12 0.5564987533474977 119 13 -0.5943168875942035 119 14 -0.891816936564851 119 15 -1.3028589524387755 119 16 -0.9872158187284228 119 17 -0.4269558406112921 119 18 -0.7611134896849379 119 19 -1.0567686748358867 119 20 -2.333084539176973 119 21 -2.5143493061560207 119 22 -2.6148684648684863 119 23 -1.7525576664232148 119 24 -2.7011591116633156 119 25 -2.2063926503002236 119 26 -1.4964143699437926 119 27 -1.3432678665871904 119 28 -1.1961013309752602 119 29 -1.7572025546815553 119 30 -1.10774517266114 119 31 -1.0731346851094727 119 32 -1.04383898081376 119 33 -0.5678370648958833 119 34 -0.6938142623016716 119 35 -0.1606919690953319 119 36 0.2199258724468791 119 37 -1.4715641501355847 119 38 -0.3049436091453046 119 39 -0.3782204723381284 119 40 0.13578494334797853 119 41 -6.129920686223198 119 42 -6.013924427579884 119 43 -5.361084876841172 119 44 -5.0338288462238125 119 45 -4.953279890044602 119 46 -3.8368738870839643 119 47 -4.224317815704374 119 48 -4.0819141409477595 119 49 -3.3966090165669947 119 50 -4.325376840712973 119 51 -3.4010788697763488 119 52 -3.715428549041988 119 53 -2.508183623099601 119 54 -2.026966264361228 119 55 -4.603242392528099 119 56 -4.692926587884571 119 57 -4.338604742596338 119 58 -5.858493164165441 119 59 -5.289068168059753 119 60 -3.3904300881191602 119 61 -2.9658154637146303 119 62 -2.5825235921874636 119 63 -2.3908766536917874 119 64 -2.2214539662018566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 2.0 6 0.0 7 8.0 8 10.0 9 8.0 10 6.0 11 11.0 12 10.0 13 23.0 14 23.0 15 16.0 16 30.0 17 34.0 18 34.0 19 45.0 20 57.0 21 71.0 22 79.0 23 102.0 24 100.0 25 106.0 26 126.0 27 148.0 28 217.0 29 206.0 30 279.0 31 338.0 32 384.0 33 525.0 34 650.0 35 767.0 36 942.0 37 1484.0 38 2470.0 39 2631.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.47002758966642 1.3711228158180755 0.07524454477050413 0.0836050497450046 8 99.27160080375084 0.5693235097119892 0.06697923643670461 0.09209645010046885 9 97.20759133851683 1.6470194799765907 0.509990803444528 0.6353983780620349 10 36.242789064459494 42.92283253908536 7.900677200902935 12.93370119555221 11 23.242203829111276 38.29111278321211 19.864559819413092 18.602123568263522 12 38.525206922498114 16.294624195301395 24.956107348883872 20.22406153331661 13 24.830699774266364 15.776272886882367 32.17122314187777 27.221804196973498 14 22.305827271967228 29.86372376891564 26.185101580135438 21.64534737898169 15 33.450380402976336 21.48649778446618 24.822339269291867 20.240782543265613 16 23.41777443357579 25.59986623192041 29.29520943064961 21.68714990385419 17 27.656550455647523 24.70529219964886 23.04155170972327 24.596605634980353 18 27.12983864225399 29.236685895828106 21.227322130256667 22.406153331661233 19 20.93470445614915 26.068054510492434 29.98077083855865 23.016470194799766 20 19.914722849260095 29.1530808460831 22.038291112783213 28.893905191873586 21 22.87434161023326 25.65002926176741 27.180001672100996 24.295627455898337 22 22.489758381406237 27.07967561240699 26.35231167962545 24.078254326561325 23 27.65886287625418 30.292642140468228 23.18561872909699 18.862876254180602 24 22.94958615500376 35.42345957695845 21.720591923752195 19.906362344285593 25 21.61551969228196 27.351785266326615 24.55054770465758 26.482147336733842 26 24.715623954499833 28.60488457678153 25.309468049514887 21.370023419203747 27 20.832984862423682 26.494940202391902 27.05528142510663 25.61679351007778 28 16.213502886304692 32.26804986195934 30.042667112858695 21.47578013887727 29 20.257719019328928 24.257384319303824 33.93021504476613 21.55468161660112 30 15.329261149694586 31.269349845201237 35.71249267843695 17.688896326667226 31 16.29425056490083 26.311825257343713 39.45936898485229 17.934555192903172 32 14.755607633076664 26.322397053900232 42.8439906260462 16.0780046869769 33 18.588294398392364 25.26165954952692 39.395461776773004 16.754584275307714 34 16.79095098449937 29.551738583996652 39.564306661080856 14.093003770423126 35 12.598491198658843 35.004191114836544 39.92455993294216 12.472757753562448 36 15.822996982903117 27.329869259135098 41.17499161917533 15.672142138786457 37 16.549295774647888 26.349765258215964 44.93628437290409 12.16465459423206 38 12.687631027253667 29.744234800838576 45.509433962264154 12.058700209643606 39 13.922670468841735 28.725991780592132 44.275769521093686 13.075568229472449 40 11.549106768430764 29.287930889876705 44.183510861360396 14.979451480332132 41 13.265648598758181 26.682329249874144 43.027353582815905 17.02466856855177 42 13.494461228600201 28.222557905337364 42.30446458543135 15.978516280631084 43 13.840859493033406 27.068994460298807 45.920765485982876 13.16938056068491 44 12.841185857058873 26.47182329722012 46.77080708826741 13.916183757453599 45 16.453781512605044 25.554621848739494 41.66386554621849 16.327731092436977 46 13.743279569892472 25.865255376344088 45.83333333333333 14.558131720430106 47 14.649307013859723 27.21545569088618 45.77908441831163 12.356152876942462 48 16.458035789296815 28.345795177686295 41.83819205242376 13.357976980593127 49 13.047130975384357 28.312190204150212 44.92984961774343 13.710829202722003 50 11.992604420539541 27.52332128750315 46.415665181948064 14.068409110009245 51 13.00327813734555 27.31781121291082 43.725308901403714 15.953601748339919 52 14.116361190516228 28.35042878762401 43.80359845300151 13.729611568858246 53 14.038332212508406 28.42972427706792 42.30833893745797 15.223604572965701 54 14.439401580097497 27.618087073457726 42.956799462094466 14.98571188435031 55 16.43973777105396 28.878803160194995 39.04857959320894 15.632879475542108 56 16.58680117696511 29.735182849936947 40.159730979403115 13.518284993694829 57 19.024800336275746 29.113072719630097 38.94913829340059 12.912988650693569 58 17.18658034137728 32.632641049356764 36.45842091986883 13.722357689397125 59 13.944290162416898 34.402087015063536 38.12168644281747 13.531936379702096 60 17.22166162125799 33.60242179616549 35.61217625294316 13.563740329633367 61 15.121951219512194 32.548359966358284 38.83936080740118 13.490328006728344 62 13.110756033975276 34.75737953073753 36.32999747708351 15.801866958203684 63 16.583971070557567 32.579261626440164 34.63964342780254 16.197123875199733 64 14.896482073724963 34.39656623464064 37.359030466251475 13.347921225382933 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 2.0 8 2.0 9 2.0 10 14.0 11 26.0 12 37.0 13 76.5 14 105.0 15 82.0 16 101.0 17 143.0 18 154.5 19 166.0 20 224.5 21 306.5 22 330.0 23 413.0 24 511.5 25 527.0 26 555.0 27 550.0 28 517.0 29 581.0 30 518.5 31 392.0 32 470.0 33 548.0 34 431.0 35 274.0 36 234.0 37 203.0 38 187.0 39 202.0 40 205.0 41 207.0 42 206.0 43 232.5 44 259.0 45 262.5 46 288.5 47 311.0 48 326.5 49 304.5 50 267.0 51 362.0 52 537.5 53 618.0 54 519.5 55 421.5 56 422.0 57 462.0 58 502.0 59 412.5 60 377.5 61 432.0 62 353.0 63 236.5 64 199.0 65 190.5 66 159.0 67 136.0 68 122.5 69 109.0 70 84.5 71 66.0 72 72.0 73 54.5 74 29.0 75 21.0 76 19.5 77 13.0 78 8.0 79 8.5 80 5.0 81 1.0 82 1.0 83 1.0 84 1.0 85 0.5 86 0.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.14212858456650782 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.008360504974500459 24 0.0 25 0.016721009949000918 26 0.0418025248725023 27 0.033442019898001836 28 0.06688403979600367 29 0.0836050497450046 30 0.0836050497450046 31 0.1003260596940055 32 0.10868656466850597 33 0.15048908954100826 34 0.21737312933701194 35 0.2591756542095142 36 0.24245464426051333 37 0.27589666415851516 38 0.3009781790820165 39 0.31769918903101746 40 0.31769918903101746 41 0.35950171390351976 42 0.3762227238525207 43 0.39294373380152164 44 0.4514672686230248 45 0.509990803444528 46 0.4765487835465262 47 0.4681882785720257 48 0.48490928852102666 49 0.48490928852102666 50 0.5183513084190284 51 0.5350723183680294 52 0.5601538332915308 53 0.5434328233425298 54 0.5267118133935289 55 0.5267118133935289 56 0.5517933283170303 57 0.5517933283170303 58 0.5685143382660313 59 0.6521193880110359 60 0.5768748432405317 61 0.5935958531895327 62 0.5852353482150322 63 0.5852353482150322 64 0.6604798929855363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 11961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.91873589164786 #Duplication Level Percentage of deduplicated Percentage of total 1 73.9291465378422 38.38307833793161 2 12.028985507246377 12.490594431903688 3 4.99194847020934 7.775269626285428 4 3.140096618357488 6.521193880110358 5 1.6586151368760065 4.305660061867736 6 1.497584541062802 4.6651617757712565 7 0.5475040257648953 1.9898001839311095 8 0.5152979066022545 2.1402892734721175 9 0.3864734299516908 1.805869074492099 >10 1.1755233494363928 10.132932029094558 >50 0.032206119162640906 1.3794833207925759 >100 0.0966183574879227 8.410668004347462 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA 246 2.056684223727113 No Hit GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 245 2.048323718752613 No Hit GAACGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAA 155 1.2958782710475714 No Hit GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 1.1704706964300644 No Hit GAACGCGGGGGTCCCTTCCCTCCAGGGCTATCCCATCTGCTGCCCTACTTCTACGTCCTCTACT 119 0.9949000919655547 No Hit GAACGCGGGAACCATTACCAGATGAAAAACCCAAATGGCCCGTACCCATACACTCTGAAGTTGT 101 0.8444110024245465 No Hit GAACGCGGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCTGATAATGT 86 0.7190034278070395 No Hit GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG 79 0.6604798929855363 No Hit GAACGCGGGATGTGGACCCGAGTAGATCCATAATCATGGAAGCAGCACGTGGCACCTGCATCTT 50 0.41802524872502295 No Hit GAACGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.401304238776022 No Hit GAACGCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGA 45 0.3762227238525207 No Hit GAACGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.2675361591840147 No Hit GAACGCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAG 31 0.2591756542095142 No Hit GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.2591756542095142 No Hit GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.2508151492350138 No Hit GAACGCGGGCCTTGTATTCGTTTGTGAGTATTTACGGATTATGTACTCTTCTGTTCCTCGTGCA 30 0.2508151492350138 No Hit GAACGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 25 0.20901262436251147 No Hit GAACGCGGGTACTTTTTAAAGTTAAGTTTATGTTCTTCCAGAACCTAGTGTCTTAAAGTTGCCT 25 0.20901262436251147 No Hit GAACGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG 22 0.18393110943901012 No Hit GAACGCGGGAGCTGTTGTAGAATTCCGTAGTTCCCCATGCAGCCCGTTGCTCTCCTGGCTTCTC 22 0.18393110943901012 No Hit GAACGCGGGCACTGAGCTACATTGCCAGCCTCAAGAGCTTCAGGATCGACATCCAGAGAATGGA 21 0.17557060446450964 No Hit GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGACTCTGGAAACAGACAAACCT 21 0.17557060446450964 No Hit GAACGCGGGAGTTTGGAAGCAGCAGTGAATTCAGAGAAGGTTCCTGTAAAGTCCATTTCTCTGT 21 0.17557060446450964 No Hit GAACGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG 20 0.1672100994900092 No Hit GAACGCGGGTACTACAGCCTCGTGCTGAAGTGTTATCACTAAACTGTCACCTTTGCTCCTATTC 19 0.15884959451550873 No Hit GAACGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.15884959451550873 No Hit GAACGCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.15048908954100826 No Hit GAACGCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATG 18 0.15048908954100826 No Hit GAACGCGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCTCAAAACCC 17 0.14212858456650782 No Hit GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.14212858456650782 No Hit GAACGCGGGGGGTGGGGCCCAGTCCGAACCTGGCCCTCCCGCCCCCATCTGCCTTTAGGTCGCT 17 0.14212858456650782 No Hit GAACGCGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCAT 17 0.14212858456650782 No Hit GAACGCGGGATCCAGAAGGTAATTTTTAAAGTTCTGAGGGCCTCCCTTTCTGCTTCAGGAGAAA 16 0.13376807959200734 No Hit GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.13376807959200734 No Hit GAACGCGGGAGCGAACCCGGGGAACTGAAACATCTAAGTACCCGGAGGAAAGGACATCAAACGA 16 0.13376807959200734 No Hit GAACGCAGGACTTAAAGGCTCTTAATACCACCTACCAATCCCAGCTCATCAAACCCAGCCGCAT 16 0.13376807959200734 No Hit GAACGCGGGACTATGAGGCTCTGAGCCTCTCTGTGCCTTGGGCCCTAAAGCCCCATGTGTAGTA 15 0.1254075746175069 No Hit GAACGCGGGGTGCCTACCTTCTCACTCCTGTTGTCCTGCCTCGGTTTGCCCAATTGTCCTCATC 15 0.1254075746175069 No Hit GAACGCGGGAGTTACTTCTCCATCTCAACTAAGGTGTAAAGTGGGTTTGCCCCACTACTGCAAG 15 0.1254075746175069 No Hit GAACGCGGGGGCCATCTTGCGTCCCCGCGTGTGTGCGCCTTCTCTCAGCTGGTCTGCCCGAGAC 15 0.1254075746175069 No Hit GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA 15 0.1254075746175069 No Hit GAACGCGGGACTGCACAATAGCTCCTCCCAGGGTTTTAACTTTGTTTTATTTTCAAAACCAGGT 14 0.11704706964300643 No Hit GAACGCGGGATCATTGGGTCACCTCAAGTTTGCCTGTGTACAGCTCTGCTTGGCCATGAGCCGC 14 0.11704706964300643 No Hit GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.11704706964300643 No Hit GAACGCGGGATGTGGGATGTTATTTGTCCTGGGCAGATTTCCATATCTGATGGTTGTCAGCGCT 14 0.11704706964300643 No Hit GAACGCGGGAAGCAGTGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.11704706964300643 No Hit GAACGCGGGGGGATGAGTCTCCGGGTGGGTCAGGAGCAAACCTCCCTGACCAGTGTTCAATGCT 13 0.10868656466850597 No Hit GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 13 0.10868656466850597 No Hit GAACGCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.10868656466850597 No Hit GAACGCGGGATGGCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.1003260596940055 No Hit GAACGCGGGGTTGCGAAAGAGCCCCCTGCTCCAAGTTTTCGTCCATCAGAGCAGCATATTCCTC 12 0.1003260596940055 No Hit GAACGCGGGGTCTTTGGTTCGTCCTCAGTGCAGGGCAACAGGACTTTGGGCTCAGGATGGTGGC 12 0.1003260596940055 No Hit GAACGCGGGAAAGAAATTCTGTGGTACTTGTTTGTTTTGTTACTGTTTTTATTCAGGTTTTGGT 12 0.1003260596940055 No Hit GAACGCGGGTGATGTAGGGCTCTGGATGGTGGCACCGCCTGGAAGAGGCGTGAGGCGTGTTGAA 12 0.1003260596940055 No Hit GAACGCGGGCAGTACGCAGAGTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.1003260596940055 No Hit GAACGCGGGCAGCTACTTTTCCACAGGCTGTCTCTGAAAAGTTCCTGTAGATATTACAGGGATG 12 0.1003260596940055 No Hit GAACGCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.1003260596940055 No Hit GAACGCGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCAACATGCC 12 0.1003260596940055 No Hit GAACGCGGGGGTTTCCTCTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.1003260596940055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.008360504974500459 0.0 0.0 0.0 0.0 11 0.02508151492350138 0.0 0.0 0.0 0.0 12 0.02508151492350138 0.0 0.0 0.0 0.0 13 0.033442019898001836 0.0 0.0 0.0 0.0 14 0.05852353482150322 0.0 0.0 0.0 0.0 15 0.05852353482150322 0.0 0.0 0.0 0.0 16 0.05852353482150322 0.0 0.0 0.0 0.0 17 0.05852353482150322 0.0 0.0 0.0 0.0 18 0.05852353482150322 0.0 0.0 0.0 0.0 19 0.05852353482150322 0.0 0.0 0.0 0.0 20 0.06688403979600367 0.0 0.0 0.0 0.0 21 0.06688403979600367 0.0 0.0 0.0 0.0 22 0.09196555471950506 0.0 0.0 0.0 0.0 23 0.10032605969400551 0.0 0.0 0.0 0.0 24 0.10032605969400551 0.0 0.0 0.0 0.0 25 0.10868656466850597 0.0 0.0 0.0 0.0 26 0.10868656466850597 0.0 0.0 0.0 0.0 27 0.1254075746175069 0.0 0.0 0.0 0.0 28 0.18393110943901012 0.0 0.0 0.0 0.0 29 0.18393110943901012 0.0 0.0 0.0 0.0 30 0.1922916144135106 0.0 0.0 0.0 0.0 31 0.20065211938801103 0.0 0.0 0.0 0.0 32 0.20065211938801103 0.0 0.0 0.0 0.0 33 0.2090126243625115 0.0 0.0 0.0 0.0 34 0.21737312933701194 0.0 0.0 0.0 0.0 35 0.2675361591840147 0.0 0.0 0.0 0.0 36 0.27589666415851516 0.0 0.0 0.0 0.0 37 0.27589666415851516 0.0 0.0 0.0 0.0 38 0.28425716913301563 0.0 0.0 0.0 0.0 39 0.2926176741075161 0.0 0.0 0.0 0.0 40 0.3009781790820166 0.0 0.0 0.0 0.0 41 0.309338684056517 0.0 0.0 0.0 0.0 42 0.309338684056517 0.0 0.0 0.0 0.0 43 0.3344201989800184 0.0 0.0 0.0 0.0 44 0.3427807039545189 0.0 0.0 0.0 0.0 45 0.3427807039545189 0.0 0.0 0.0 0.0 46 0.3511412089290193 0.0 0.0 0.0 0.0 47 0.35950171390351976 0.0 0.0 0.0 0.0 48 0.3762227238525207 0.0 0.0 0.0 0.0 49 0.3845832288270212 0.0 0.0 0.0 0.0 50 0.3845832288270212 0.0 0.0 0.0 0.0 51 0.3845832288270212 0.0 0.0 0.0 0.0 52 0.3845832288270212 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGAGT 15 0.0044956123 58.253166 43 GTTTGAT 15 0.0044956123 58.253166 48 TGATCAT 15 0.0044956123 58.253166 51 ACGTAGA 15 0.0044956123 58.253166 41 TCATGGC 15 0.0044956123 58.253166 54 TTGATCA 15 0.0044956123 58.253166 50 TAGAGTT 15 0.0044956123 58.253166 44 ATCATGG 15 0.0044956123 58.253166 53 CATGGCT 15 0.0044956123 58.253166 55 AGTTTGA 15 0.0044956123 58.253166 47 TTTGATC 15 0.0044956123 58.253166 49 GTGTCTC 15 0.004571667 58.008404 25 AAACTGG 20 1.928881E-4 58.008404 39 GGGCAAA 20 1.928881E-4 58.008404 35 TGCGTGT 15 0.004571667 58.008404 22 TTCCGGG 20 1.928881E-4 58.008404 31 TGTCTCC 15 0.004571667 58.008404 26 CAGTACG 15 0.004571667 58.008404 37 GGCAAAC 20 1.928881E-4 58.008404 36 GCGTGTC 15 0.004571667 58.008404 23 >>END_MODULE