FastQCFastQC Report
Fri 17 Jun 2016
SRR1526836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12022
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA2642.195974047579438No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2161.796706038928631No Hit
GAACGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAA1581.3142571951422393No Hit
GAACGCGGGGGTCCCTTCCCTCCAGGGCTATCCCATCTGCTGCCCTACTTCTACGTCCTCTACT1481.2310763600066543No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.9732157710863417No Hit
GAACGCGGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCTGATAATGT820.682082848111795No Hit
GAACGCGGGAACCATTACCAGATGAAAAACCCAAATGGCCCGTACCCATACACTCTGAAGTTGT740.6155381800033272No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG720.5989020129762103No Hit
GAACGCGGGATGTGGACCCGAGTAGATCCATAATCATGGAAGCAGCACGTGGCACCTGCATCTT520.4325403427050407No Hit
GAACGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.4242222591914823No Hit
GAACGCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGA420.349359507569456No Hit
GAACGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC300.2495425054067543No Hit
GAACGCGGGTACTTTTTAAAGTTAAGTTTATGTTCTTCCAGAACCTAGTGTCTTAAAGTTGCCT300.2495425054067543No Hit
GAACGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG280.23290633837963734No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.21627017135252036No Hit
GAACGCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAG250.20795208783896188No Hit
GAACGCGGGCACTGAGCTACATTGCCAGCCTCAAGAGCTTCAGGATCGACATCCAGAGAATGGA250.20795208783896188No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1996340043254034No Hit
GAACGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1996340043254034No Hit
GAACGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.19131592081184495No Hit
GAACGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG230.19131592081184495No Hit
GAACGCGGGCCTTGTATTCGTTTGTGAGTATTTACGGATTATGTACTCTTCTGTTCCTCGTGCA230.19131592081184495No Hit
GAACGCGGGAGTTTGGAAGCAGCAGTGAATTCAGAGAAGGTTCCTGTAAAGTCCATTTCTCTGT230.19131592081184495No Hit
GAACGCAGGCGGCTACGGGAGCTCTTCAAGCCCAGCATGGCCGAGCTGGGACTCTGTATCTACC220.18299783729828648No Hit
GAACGCGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCTCAAAACCC210.174679753784728No Hit
GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.16636167027116952No Hit
GAACGCGGGAGCGTCAAGAGCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.15804358675761104No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGACTCTGGAAACAGACAAACCT190.15804358675761104No Hit
GAACGCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATG190.15804358675761104No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA190.15804358675761104No Hit
GAACGCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.14972550324405257No Hit
GAACGCGGGTACTACAGCCTCGTGCTGAAGTGTTATCACTAAACTGTCACCTTTGCTCCTATTC180.14972550324405257No Hit
GAACGCGGGGTGCCTACCTTCTCACTCCTGTTGTCCTGCCTCGGTTTGCCCAATTGTCCTCATC170.1414074197304941No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1414074197304941No Hit
GAACGCGGGAGCTGTTGTAGAATTCCGTAGTTCCCCATGCAGCCCGTTGCTCTCCTGGCTTCTC170.1414074197304941No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC160.1330893362169356No Hit
GAACGCGGGGGGATGAGTCTCCGGGTGGGTCAGGAGCAAACCTCCCTGACCAGTGTTCAATGCT160.1330893362169356No Hit
GAACGCGGGATGGCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1330893362169356No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12477125270337715No Hit
GAACGCGGGTGATGTAGGGCTCTGGATGGTGGCACCGCCTGGAAGAGGCGTGAGGCGTGTTGAA150.12477125270337715No Hit
GAACGCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12477125270337715No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12477125270337715No Hit
GAACGCGGGATCCAGAAGGTAATTTTTAAAGTTCTGAGGGCCTCCCTTTCTGCTTCAGGAGAAA140.11645316918981867No Hit
GAACGCGGGATCATTGGGTCACCTCAAGTTTGCCTGTGTACAGCTCTGCTTGGCCATGAGCCGC140.11645316918981867No Hit
GAACGCGGGCAAACCATACTGAATTTAGGTGGATAAGGGCTAGAAATTTTGAGCAACTAAATTT140.11645316918981867No Hit
GAACGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG130.10813508567626018No Hit
GAACGCGGGACGCACACACACAAACATCTGTATACCTCTGAATTTACTACAGACTCTAGGTTCT130.10813508567626018No Hit
GAACGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10813508567626018No Hit
GAACGCGGGGTTGCGAAAGAGCCCCCTGCTCCAAGTTTTCGTCCATCAGAGCAGCATATTCCTC130.10813508567626018No Hit
GAACGCGGGAACATGTGTGTTGTGTAGAGCTGTTGCCGGTTTGGTCATGCCTGGTTTCTTATGT130.10813508567626018No Hit
GAACGCGGGATACGTACCGCGCGTGATTGACGTAACGGCTGGGATCGGCATCCCTTACAGCGAG130.10813508567626018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAC201.8500662E-458.49789442
TGCCCTA201.8500662E-458.49789441
GCTGCCC201.8500662E-458.49789439
CTGCCCT201.8500662E-458.49789440
CCCTACT201.8500662E-458.49789443
TTGTCAT150.00442160258.4978944
CCTATTG150.00442160258.4978940
TCCTATT150.00442160258.4978939
TGTCATC150.00442160258.4978945
TATTGTC150.00442160258.4978942
ATTGTCA150.00442160258.4978943
CTATTGT150.00442160258.4978941
TCTGGCG150.004496407758.252120
AGGGCTA303.3513788E-758.252124
CTCCAGG303.3513788E-758.252120
CAGGGCT303.3513788E-758.252123
GCGCCAC150.004496407758.252126
CGCGCCA150.004496407758.252125
ATCCTAT150.004572151758.0083738
ATGGGCC150.004572151758.0083752
Produced by FastQC (version 0.11.5)