FastQCFastQC Report
Fri 17 Jun 2016
SRR1526835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2402.2742348147446223No Hit
GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG2212.0941912252440065No Hit
GAACGCGGGGGTCCCTTCCCTCCAGGGCTATCCCATCTGCTGCCCTACTTCTACGT1601.5161565431630817No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1411.3361129536624656No Hit
GAACGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGG1391.3171609968729272No Hit
GAACGCGGGAACCATTACCAGATGAAAAACCCAAATGGCCCGTACCCATACACTCT920.871790012318772No Hit
GAACGCGGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCT850.8054581635553871No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC750.7106983796076944No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGA550.5211788117123093No Hit
GAACGCGGGATGTGGACCCGAGTAGATCCATAATCATGGAAGCAGCACGTGGCACC490.46432294134369373No Hit
GAACGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.41694304936984744No Hit
GAACGCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCT380.3600871790012319No Hit
GAACGCGGGAGTTTGGAAGCAGCAGTGAATTCAGAGAAGGTTCCTGTAAAGTCCAT350.33165924381692413No Hit
GAACGCGGCAGGGCAGGGCAATAGTCCCTGTGTCTAGGCCTGGGATGAGGAAGGGG340.32218326542215486No Hit
GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.3127072870273856No Hit
GAACGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG330.3127072870273856No Hit
GAACGCGGGCCTTGTATTCGTTTGTGAGTATTTACGGATTATGTACTCTTCTGTTC280.2653273950535393No Hit
GAACGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.25585141665877004No Hit
GAACGCGGGGGGTGGGGCCCAGTCCGAACCTGGCCCTCCCGCCCCCATCTGCCTTT270.25585141665877004No Hit
GAACGCGGGTACTTTTTAAAGTTAAGTTTATGTTCTTCCAGAACCTAGTGTCTTAA220.20847152468492372No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.19899554629015445No Hit
GAACGCGGGAGTATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCT210.19899554629015445No Hit
GAACGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.18004358950061594No Hit
GAACGCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.18004358950061594No Hit
GAACGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA190.18004358950061594No Hit
GAACGCGGGTACTACAGCCTCGTGCTGAAGTGTTATCACTAAACTGTCACCTTTGC190.18004358950061594No Hit
GAACGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG180.17056761110584667No Hit
GAACGCGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT180.17056761110584667No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.16109163271107743No Hit
GAACGCGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACA170.16109163271107743No Hit
GAACGCAGGACTTAAAGGCTCTTAATACCACCTACCAATCCCAGCTCATCAAACCC170.16109163271107743No Hit
GAACGCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT160.15161565431630816No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGACTCTGGAAACAG160.15161565431630816No Hit
GAACGCGGGACGCACACACACAAACATCTGTATACCTCTGAATTTACTACAGACTC160.15161565431630816No Hit
GAACGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG150.1421396759215389No Hit
GAACGCAGGCGGCTACGGGAGCTCTTCAAGCCCAGCATGGCCGAGCTGGGACTCTG150.1421396759215389No Hit
GAACGCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC150.1421396759215389No Hit
GAACGCAGGAAGCAGTGGTAATGTAACACCATCCTGTTGAGATTCAGATTAGTCCT150.1421396759215389No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.13266369752676965No Hit
GAACGCGGGTGATGTAGGGCTCTGGATGGTGGCACCGCCTGGAAGAGGCGTGAGGC140.13266369752676965No Hit
GAACGCGGGAAGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.13266369752676965No Hit
GAACGCGGGATTTCAAACAAAACCAAATGCTGTCGTCGACAAGCCAGCACAGGCGA140.13266369752676965No Hit
GAACGCGGGCAAACCATACTGAATTTAGGTGGATAAGGGCTAGAAATTTTGAGCAA140.13266369752676965No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12318771913200038No Hit
GAACGCGGGGTCTTTGGTTCGTCCTCAGTGCAGGGCAACAGGACTTTGGGCTCAGG130.12318771913200038No Hit
GAACGCGGGGTTGCGAAAGAGCCCCCTGCTCCAAGTTTTCGTCCATCAGAGCAGCA130.12318771913200038No Hit
GAACGCGGGAGCTGTTGTAGAATTCCGTAGTTCCCCATGCAGCCCGTTGCTCTCCT130.12318771913200038No Hit
GAACGCGGGACTATGAGGCTCTGAGCCTCTCTGTGCCTTGGGCCCTAAAGCCCCAT130.12318771913200038No Hit
GAACGCGGGGTACTACATTTTTATTTTCTAACCGTTAAACACTGTATTTGAGTTTT130.12318771913200038No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC130.12318771913200038No Hit
GAACGCGGGGGGGTCCGTCTGAGTCCTGGCCGAGGAACTTCTGTTGGTGTTTGGGG130.12318771913200038No Hit
GAACGCGGGATGTGGGATGTTATTTGTCCTGGGCAGATTTCCATATCTGATGGTTG130.12318771913200038No Hit
GAACGCGGGCAGTACGCAGAGTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.12318771913200038No Hit
GAACGCGGGTGTGCTCAATTTAATGCCTAGCAGTCTCCTCCTGACTGGTTTTCCTG130.12318771913200038No Hit
GAACGCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAG130.12318771913200038No Hit
GAACGCGGGAAGCATGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT120.11371174073723112No Hit
GAACGCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGGGCTCTCTGG120.11371174073723112No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11371174073723112No Hit
GAACGCGGGGCAGTGCTGAACGCGGGAAGCATGGTATAACGAGAGTCGACTTTTTT120.11371174073723112No Hit
GAACGCGGGCACTGAGCTACATTGCCAGCCTCAAGAGCTTCAGGATCGACATCCAG120.11371174073723112No Hit
GAACGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA120.11371174073723112No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC120.11371174073723112No Hit
GAACGCGGGACAGTGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT120.11371174073723112No Hit
GAACGCGGGACTGCACAATAGCTCCTCCCAGGGTTTTAACTTTGTTTTATTTTCAA120.11371174073723112No Hit
GAACGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG110.10423576234246186No Hit
GAACGCGGGATCCAGAAGGTAATTTTTAAAGTTCTGAGGGCCTCCCTTTCTGCTTC110.10423576234246186No Hit
GAACGCGGGTGGTTATTTGCTGTGGACTTAAAATACCGTCCTGAATATTCCTTGCC110.10423576234246186No Hit
GAACGCGGGGTGGATTTCTGCATAATTTACTAGTGCATCCTGTTCAGCATCCCTTT110.10423576234246186No Hit
GAACGCGGGAGTGGGAAATGGCGTCTCGGGAGTCAGGCGGCTCTCGGGCGGCCGCC110.10423576234246186No Hit
GAACGCGGGGTTTGAACGGAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGC110.10423576234246186No Hit
GAACGCGGGAGCGAACCCGGGGAACTGAAACATCTAAGTACCCGGAGGAAAGGACA110.10423576234246186No Hit
GAACGCGGGAGGCGGTTTCAGAGTGAATAGGGACTGGCCACTTCCAAGGCGAGCAC110.10423576234246186No Hit
GAACGCGGGATCATTGGGTCACCTCAAGTTTGCCTGTGTACAGCTCTGCTTGGCCA110.10423576234246186No Hit
GAACGCGGGAAGTGGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT110.10423576234246186No Hit
GAACGCGGGACAGTGGTCAACGCTTTGTAACTTTTGATTCTTTTTTTATTCTGAAA110.10423576234246186No Hit
GAACGCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10423576234246186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCAT150.00783593250.57692750
GAGCGAC251.819191E-550.57692350
CGAGCGA251.8721985E-550.3349349
TTCTACG203.86554E-450.3349349
CCGAGCG251.8721985E-550.3349348
AAAGTCC150.00798700950.3349348
CTTCTAC203.86554E-450.3349348
AAGTCCA150.00798700950.3349349
GCCCTAC251.9264793E-550.09523842
ATCCCAT251.9264793E-550.09523830
TGGTCCA251.9264793E-550.09523839
TCCCATC251.9264793E-550.09523831
CCTACTT251.9264793E-550.09523844
AGAAGGT150.00814024450.09523835
GGCGCGT203.9585689E-450.09523833
TCAGGGC251.9264793E-550.09523826
CCTGTAA150.00814024450.09523843
CACGCCG251.9264793E-550.09523844
CCACGCC251.9264793E-550.09523843
GGTCCAC251.9264793E-550.09523840
Produced by FastQC (version 0.11.5)