FastQCFastQC Report
Fri 17 Jun 2016
SRR1526834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67217
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2930.4359016320276121No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.3897823467277623No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.2439858964250115No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.22762098873796804No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.19340345448324084No Hit
TCAAAGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCC730.10860347828674294No Hit
TCAAAGGGGTAAATCATCTATACAAAAGTCTTGGGTGTTAATTTTAATATTTTGAGCAAAATCT680.10116488388354136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAAC150.00446605458.65079558
AAACCCG150.00446605458.65079558
AAAATCA150.00446605458.65079556
TAAAACT150.004628779458.12359650
CTGATGT150.004628779458.12359650
ATAGACC150.004642536458.0800944
ATATGAA150.004642536458.0800940
AATATGA150.004642536458.0800939
ATCGTGT150.004642536458.0800940
TATGAAG201.9830602E-458.0800941
TGTTGCC150.004642536458.0800939
ATATCTG150.004642536458.0800948
TATCTGC150.004642536458.0800949
CTAAAAC150.004642536458.0800949
ATCCCGC150.004642536458.0800944
GATATCT150.004642536458.0800947
CTCTAAA150.004642536458.0800947
AATCGTG150.004642536458.0800939
GTGTTGC150.004656323758.03664838
AATTTGG150.004656323758.03664832
Produced by FastQC (version 0.11.5)