FastQCFastQC Report
Fri 17 Jun 2016
SRR1526833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92558
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6980.7541217398820199No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.400829749994598No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3670.39650813543940017No Hit
TCAAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2580.2787441388102595No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.21932193867628944No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.21716113139869053No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1870.20203548045549818No Hit
TCAAAGGGGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGT1820.1966334622615009No Hit
TCAAAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1660.1793470040407096No Hit
TCAAAGGGGGTGACATGGCTGGCAATTCTGCTTTATAAAATCGTGTCTCTAAAACTGTGTTCAT1650.17826660040191014No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1610.17394498584671234No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.16854296765271504No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1550.1674625640139156No Hit
TCAAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1530.1653017567363167No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1520.16422135309751723No Hit
TCAAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1480.1598997385423194No Hit
TCAAAGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCC1440.1555781239871216No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1430.15449772034832213No Hit
TCAAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1410.15233691307072322No Hit
TCAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1370.1480152985155254No Hit
TCAAAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC1200.12964843665593465No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1180.12748762937833574No Hit
TCAAAGGGGGTATGGCAAGAGGGTGATTCAGAAGGCCCTGGAGGAGATGGAAAGTAAAGACTGG1160.12532682210073684No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1160.12532682210073684No Hit
TCAAAGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1130.12208561118433849No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1120.121005207545539No Hit
TCAAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1020.11020117115754446No Hit
TCAAAGGGGGGAGTTATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGA1010.10912076751874501No Hit
TCAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG980.10587955660234663No Hit
TCAAAGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT950.10263834568594826No Hit
TCAAAGGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC940.10155794204714881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGAC150.00458856258.26946355
TGTATGC150.00458856258.26946353
GTGGATT150.00458856258.26946356
GCACAAG303.606092E-758.26946356
ATTCAGC150.00458856258.26946358
GCTAAGT150.00458856258.26946358
CAAAATC201.9552896E-458.2694657
TGCGAGC201.9552896E-458.2694658
CCGAGGT406.7848305E-1058.2694658
AAAATCT201.9552896E-458.2694658
TGTCAAA150.00459846758.2377652
ACTAAGT150.00459846758.2377652
ACGCCCA150.00460838858.20609348
CGCACTA150.00460838858.20609349
GCACTAA150.00460838858.20609350
TTTTACG150.00460838858.20609349
ATCTGTT150.00460838858.20609344
ATCGTTT150.00461832558.1744643
CTTATTC150.00461832558.1744642
GTCCCAC150.00461832558.1744643
Produced by FastQC (version 0.11.5)