FastQCFastQC Report
Fri 17 Jun 2016
SRR1526832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92688
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6630.7153029518384256No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3880.4186086656309339No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.41752977731745206No Hit
TCAAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2260.24382875884688418No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.19419989642672192No Hit
TCAAAGGGGGTGACATGGCTGGCAATTCTGCTTTATAAAATCGTGTCTCTAAAACTGTGTTCAT1770.19096323148627656No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.1855687899188676No Hit
TCAAAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1680.18125323666494045No Hit
TCAAAGGGGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGT1670.18017434835145865No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1660.17909546003797686No Hit
TCAAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1640.1769376834110133No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1590.17154324184360434No Hit
TCAAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1490.16075435870878646No Hit
TCAAAGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCC1470.1585965820818229No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.1575176937683411No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1420.15320214051441394No Hit
TCAAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1310.14133436906611427No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1300.1402554807526325No Hit
TCAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1270.13701881581218714No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1250.13486103918522355No Hit
TCAAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1210.1305454859312964No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1210.1305454859312964No Hit
TCAAAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC1180.12730882099085103No Hit
TCAAAGGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1140.12299326773692389No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1080.11651993785603315No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1080.11651993785603315No Hit
TCAAAGGGGGTATGGCAAGAGGGTGATTCAGAAGGCCCTGGAGGAGATGGAAAGTAAAGACTGG990.10680994303469704No Hit
TCAAAGGGGGAGTGGAGAGTGATTGGGAGTTACTTGTTCATTCAAATCTTGCACCCCCCCCCCC990.10680994303469704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA150.004518321258.4967245
CATTCAA201.9231743E-458.46473739
ATCCGTT150.00452812358.46473742
TGTTCGA150.00452812358.46473741
GCTAGGC258.208628E-658.46473739
GCGGACT150.00452812358.46473739
CGGTCCA406.5847416E-1058.46473744
ACTTTCA150.00452812358.46473743
GTGCGAA150.00452812358.46473743
CCGTTCG150.00452812358.46473744
ACGGTCC351.5201294E-858.46473743
TAATCTG150.00452812358.46473740
CAAAAGT150.00459717958.24183323
TCGTGTA150.00459717958.24183324
ATTTCTA150.00459717958.24183321
GTGCTAA258.39685E-658.2418322
TGATTGG201.959873E-458.2418320
ATGATGA150.004646982558.08365257
CGAAATC150.004646982558.08365257
CCTCGAA150.004646982558.08365254
Produced by FastQC (version 0.11.5)