SRR1526831_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85563
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	727	0.8496663277351192	No Hit
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	410	0.47917908441733	No Hit
TCAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	276	0.32256933487605627	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	252	0.2945198274955296	No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	233	0.27231396748594605	No Hit
TCAAAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT	222	0.2594579432698713	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	195	0.22790224746677884	No Hit
TCAAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	179	0.2092025758797611	No Hit
TCAAAGGGGGAAATCAAGACCCTTTTCACCAAGACACAGGGGAAGTCCCTGAAAGA	169	0.19751528113787503	No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	166	0.1940090927153092	No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159	0.1858279863959889	No Hit
TCAAAGGGGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACCTC	152	0.17764688007666865	No Hit
TCAAAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC	147	0.17180323270572562	No Hit
TCAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	146	0.170634503231537	No Hit
TCAAAGGGGGTGACATGGCTGGCAATTCTGCTTTATAAAATCGTGTCTCTAAAACT	145	0.1694657737573484	No Hit
TCAAAGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATG	130	0.15193483164451924	No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	126	0.1472599137477648	No Hit
TCAAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	123	0.14375372532519898	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	121	0.14141626637682175	No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	118	0.13791007795425592	No Hit
TCAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA	116	0.13557261900587872	No Hit
TCAAAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	115	0.1344038895316901	No Hit
TCAAAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC	113	0.13206643058331288	No Hit
TCAAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	113	0.13206643058331288	No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	110	0.12856024216074705	No Hit
TCAAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	109	0.12739151268655846	No Hit
TCAAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	108	0.12622278321236985	No Hit
TCAAAGGGGGAGTGGAGAGTGATTGGGAGTTACTTGTTCATTCAAATCTTGCACCC	107	0.12505405373818124	No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	95	0.1110293000479179	No Hit
TCAAAGGGGGGCGTGCTAGGACCACCGTGGCAGCTTGAGGGCGGCCTAGAGGCCTG	95	0.1110293000479179	No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	94	0.10986057057372929	No Hit
TCAAAGGGGCCTTCAGCATCTCCTGGGTTTTGATGCTTGGCTCTTTGCTGATCTTG	93	0.10869184109954068	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	93	0.10869184109954068	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTTCA	15	0.0079674665	50.67936	50
TTTTGAG	15	0.0079674665	50.67936	50
GATTAAG	90	0.0	50.679356	50
ATTTTCC	25	1.9254705E-5	50.619133	48
CTTGCAC	20	3.9164702E-4	50.619133	48
TTGATTG	15	0.008252459	50.231136	46
TACTCTC	15	0.008252459	50.231136	46
ATCTTGC	20	4.0682047E-4	50.231133	46
TATTTGA	15	0.008271724	50.201534	42
TGTTGGA	20	4.0800672E-4	50.201534	44
TCTACTC	15	0.008271724	50.201534	44
ACAGCCA	20	4.0800672E-4	50.201534	42
CGGTCTG	15	0.008271724	50.201534	42
GCGTCAT	15	0.008271724	50.201534	43
CGTCATG	15	0.008271724	50.201534	44
ATGTAAA	15	0.008291023	50.17197	38
TCTGCTA	15	0.008291023	50.17197	40
ACTCACG	25	2.0293895E-5	50.171967	39
CTAGACA	20	4.1038747E-4	50.142437	34
GATGTAA	15	0.008310354	50.142437	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content