FastQCFastQC Report
Fri 17 Jun 2016
SRR1526830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24105
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3131.2984857913295997No Hit
AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.8089607965152459No Hit
AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.43974279195187715No Hit
AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.3899605890894005No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.37336652146857496No Hit
AACGCCGGGAGCTTTAATGAGGAGCCTGTCGTGTTTCCCCATAAATTTATTGCCTGAGAGCTTG890.36921800456336856No Hit
AACGCCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.21987139597593858No Hit
AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.21572287907073223No Hit
AACGCCGGGAGGTAGGCATCAGTGACAGACCAGAGCAGAGATTCCACTCAAATCTGTCCTGGTG500.20742584526031943No Hit
AACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.18668326073428748No Hit
AACGCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.1783862269238747No Hit
AACGCCGGGCTGGAAACGCAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.14934660858743No Hit
AACGCCGGGGGGCAAGCCTTCACACCTCAGTTAACATGATCAAGATAACCCTCCACAGAATTGC360.14934660858743No Hit
AACGCCGGGAGGAGTGAAGCTTCTAGTTAACACCAGCTTTACTTTTCTGTGCTTGATAGCATAT340.1410495747770172No Hit
AACGCCGGGGGAACTGTGTTAGAATATAAAAATTTCCCCAAGGATTTATAAGCCCTCTATTTTA320.13275254096660444No Hit
AACGCCGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG310.12860402406139806No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.12860402406139806No Hit
AACGCCGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.11615847334577889No Hit
AACGCCGGGCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.11200995644057249No Hit
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11200995644057249No Hit
AACGCCGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.10786143953536612No Hit
AACGCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.10371292263015972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG150.00447004458.52100856
TCCTGGT150.00447004458.52100857
GTGTTTC201.9652066E-458.03333731
TAAATTT201.9652066E-458.03333742
AATCTGT150.004621099758.03333751
CTGAGAG201.9652066E-458.03333754
AAATTTA201.9652066E-458.03333743
ACAGACC150.004621099758.03333725
TGTCCTG150.004621099758.03333755
AATTTAT201.9652066E-458.03333744
GTCGTGT201.9652066E-458.03333728
CCTGTCG201.9652066E-458.03333725
TTATTGC201.9652066E-458.03333747
TGTTTCC201.9652066E-458.03333732
CCTGAGA201.9652066E-458.03333753
GTTTCCC201.9652066E-458.03333733
TCGTGTT201.9652066E-458.03333729
TATTGCC201.9652066E-458.03333748
CAGAGAT150.004621099758.03333736
TCAAATC150.004621099758.03333748
Produced by FastQC (version 0.11.5)