FastQCFastQC Report
Fri 17 Jun 2016
SRR1526829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28845
Sequences flagged as poor quality0
Sequence length64
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5361.8582076616397987No Hit
AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.7141618998093258No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.4021494193101057No Hit
AACGCCGGGAGGTAGGCATCAGTGACAGACCAGAGCAGAGATTCCACTCAAATCTGTCCTGGTG1070.37094817126018376No Hit
AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.3605477552435431No Hit
AACGCCGGGAGCTTTAATGAGGAGCCTGTCGTGTTTCCCCATAAATTTATTGCCTGAGAGCTTG930.32241289651586064No Hit
AACGCCGGGCTCGGTCACTGCTGGTCGCTTCCTGAGCCGCTGCTGGCTCTGTGTCTCTGTCCTC900.31201248049922No Hit
AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.2842780377881782No Hit
AACGCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.26001040041601664No Hit
AACGCCGGGCTGGCTGCCCTATCCGGAACTTGATTAACTCTGTAGTCCAGGCTGGCCTCAAACT740.2565435950771364No Hit
AACGCCGGGATCATTACACTGGCAACTCCGGGCAGCAATAATGGTCAGCAAGTGTGTCACACAT720.24960998439937598No Hit
AACGCCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.23574276304385508No Hit
AACGCCGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG640.22187554168833418No Hit
AACGCCGGGAGGAGTGAAGCTTCTAGTTAACACCAGCTTTACTTTTCTGTGCTTGATAGCATAT630.21840873634945399No Hit
AACGCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG620.21494193101057377No Hit
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.1906742936384122No Hit
AACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.1906742936384122No Hit
AACGCCGGGGGGCAAGCCTTCACACCTCAGTTAACATGATCAAGATAACCCTCCACAGAATTGC530.18374068296065177No Hit
AACGCCGGGGGAACTGTGTTAGAATATAAAAATTTCCCCAAGGATTTATAAGCCCTCTATTTTA500.17334026694401108No Hit
AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.16987346160513087No Hit
AACGCCGGGACTGAAGCTTGGGACGTTTGTTCTTCGGTTCACGTGCTCCTCAGCACGGTCTGTC480.16640665626625065No Hit
AACGCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCA450.15600624024961No Hit
AACGCCGGGCTGGAAACGCAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.15253943491072974No Hit
AACGCCGGGAAGGGGTCCAGCTCCTAGAATGAGAACCAAAGGATTAGCTCTCTGACTACCCAAG430.14907262957184955No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.14907262957184955No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.14907262957184955No Hit
AACGCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG390.13520540821632865No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.12133818686080777No Hit
AACGCCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.11787138152192754No Hit
AACGCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG320.11093777084416709No Hit
AACGCCGGGAAGTGACTTATGAAAGTCCTTCTTGATGTCCTCTATCATCATCATGAGAAATGTT310.10747096550528688No Hit
AACGCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.10747096550528688No Hit
AACGCCGGGATTGACTCTGAGCTCCCTCTGGAACCACATTCCGAAAGCTGTGCACTTCCGTGGT300.10400416016640666No Hit
AACGCCGGGGGGCCAGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT290.10053735482752643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGG150.00458376158.18150353
TGCACTT150.00458376158.18150351
GTGCACT150.00458376158.18150350
TGATCCT150.00458376158.18150351
GCTGGCC150.00458376158.18150351
CTGGCCT150.00458376158.18150352
GTCTCTG150.00458376158.18150353
AGGCTGG150.00458376158.18150349
TTGATCC150.00458376158.18150350
GAGAGCT150.00458376158.18150356
CTTCCGT150.00458376158.18150355
TGTGCAC150.00458376158.18150349
CTCAAAC150.00458376158.18150357
CTCTGTG150.00458376158.18150347
CTCTGTC150.00458376158.18150355
TGCTGGC150.004615596558.08014341
TAGTCCA150.004615596558.08014343
TCCAGGC150.004615596558.08014346
ACTTCCG150.004615596558.08014354
ATAAATT150.004615596558.08014341
Produced by FastQC (version 0.11.5)