FastQCFastQC Report
Fri 17 Jun 2016
SRR1526828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28624
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5121.7887087758524316No Hit
AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.6428172163219675No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.5205422023476802No Hit
AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.3458636109558412No Hit
AACGCCGGGAGGTAGGCATCAGTGACAGACCAGAGCAGAGATTCCACTCAAATCTGTCCTGGTG940.32839575181665737No Hit
AACGCCGGGAGCTTTAATGAGGAGCCTGTCGTGTTTCCCCATAAATTTATTGCCTGAGAGCTTG940.32839575181665737No Hit
AACGCCGGGCTCGGTCACTGCTGGTCGCTTCCTGAGCCGCTGCTGGCTCTGTGTCTCTGTCCTC920.32140860816098377No Hit
AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3004471771939631No Hit
AACGCCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.27249860257126884No Hit
AACGCCGGGCTGGCTGCCCTATCCGGAACTTGATTAACTCTGTAGTCCAGGCTGGCCTCAAACT770.26900503074343207No Hit
AACGCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.2515371716042482No Hit
AACGCCGGGATCATTACACTGGCAACTCCGGGCAGCAATAATGGTCAGCAAGTGTGTCACACAT680.23756288429290107No Hit
AACGCCGGGGGGCAAGCCTTCACACCTCAGTTAACATGATCAAGATAACCCTCCACAGAATTGC610.21310788149804358No Hit
AACGCCGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG570.1991335941866965No Hit
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.1991335941866965No Hit
AACGCCGGGGGAACTGTGTTAGAATATAAAAATTTCCCCAAGGATTTATAAGCCCTCTATTTTA510.1781721632196758No Hit
AACGCCGGGACTGAAGCTTGGGACGTTTGTTCTTCGGTTCACGTGCTCCTCAGCACGGTCTGTC510.1781721632196758No Hit
AACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1781721632196758No Hit
AACGCCGGGAGGAGTGAAGCTTCTAGTTAACACCAGCTTTACTTTTCTGTGCTTGATAGCATAT490.17118501956400223No Hit
AACGCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG440.15371716042481834No Hit
AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.14673001676914477No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1397428731134712No Hit
AACGCCGGGAAGGGGTCCAGCTCCTAGAATGAGAACCAAAGGATTAGCTCTCTGACTACCCAAG380.13275572945779765No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.13275572945779765No Hit
AACGCCGGGCTGGAAACGCAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.11878144214645053No Hit
AACGCCGGGGGGCCAGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT340.11878144214645053No Hit
AACGCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCA330.11528787031861376No Hit
AACGCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG330.11528787031861376No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.11528787031861376No Hit
AACGCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.11179429849077697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCA201.9090899E-458.41062543
CGTAAAA201.9090899E-458.41062544
CATATTA201.9090899E-458.41062526
CTGTAGT201.9090899E-458.41062540
GTAGTCC201.9090899E-458.41062542
GTAAAAT201.9090899E-458.41062545
TAAATTT150.00451233458.4106242
AACTGGA150.00451233458.4106240
AATTTAT150.00451233458.4106244
AACCTGC150.00451233458.4106242
ACCCGAC150.00451233458.4106241
ACCTGCT150.00451233458.4106243
ATAAATT150.00451233458.4106241
GAACTTG150.00451233458.4106226
CCCGACT150.00451233458.4106242
CCATAAA150.00451233458.4106239
CAAGGAT150.00451233458.4106239
CTGTCGT150.00451233458.4106226
CCGACTG150.00451233458.4106243
ATTTATA150.00451233458.4106244
Produced by FastQC (version 0.11.5)