SRR1526827_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24319
Sequences flagged as poor quality	0
Sequence length	56
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	487	2.0025494469344958	No Hit
AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	157	0.6455857559932563	No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	155	0.6373617336239155	No Hit
AACGCCGGGCTGGCTGCCCTATCCGGAACTTGATTAACTCTGTAGTCCAGGCTGGC	104	0.42764916320572394	No Hit
AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.4153131296517127	No Hit
AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.3659689954356676	No Hit
AACGCCGGGAGGTAGGCATCAGTGACAGACCAGAGCAGAGATTCCACTCAAATCTG	80	0.3289608947736338	No Hit
AACGCCGGGAGCTTTAATGAGGAGCCTGTCGTGTTTCCCCATAAATTTATTGCCTG	76	0.3125128500349521	No Hit
AACGCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	70	0.28784078292692955	No Hit
AACGCCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.28372877174225913	No Hit
AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.28372877174225913	No Hit
AACGCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68	0.2796167605575887	No Hit
AACGCCGGGCTCGGTCACTGCTGGTCGCTTCCTGAGCCGCTGCTGGCTCTGTGTCT	65	0.26728072700357747	No Hit
AACGCCGGGAGGAGTGAAGCTTCTAGTTAACACCAGCTTTACTTTTCTGTGCTTGA	64	0.263168715818907	No Hit
AACGCCGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC	55	0.22616061515687322	No Hit
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.22616061515687322	No Hit
AACGCCGGGGGAACTGTGTTAGAATATAAAAATTTCCCCAAGGATTTATAAGCCCT	52	0.21382458160286197	No Hit
AACGCCGGGATCATTACACTGGCAACTCCGGGCAGCAATAATGGTCAGCAAGTGTG	47	0.19326452567950983	No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.19326452567950983	No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46	0.1891525144948394	No Hit
AACGCCGGGGGGCAAGCCTTCACACCTCAGTTAACATGATCAAGATAACCCTCCAC	45	0.185040503310169	No Hit
AACGCCGGGACTGAAGCTTGGGACGTTTGTTCTTCGGTTCACGTGCTCCTCAGCAC	43	0.17681648094082816	No Hit
AACGCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	41	0.16859245857148733	No Hit
AACGCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.1644804473868169	No Hit
AACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36	0.1480324026481352	No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36	0.1480324026481352	No Hit
AACGCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	34	0.13980838027879436	No Hit
AACGCCGGGAAGGGGTCCAGCTCCTAGAATGAGAACCAAAGGATTAGCTCTCTGAC	34	0.13980838027879436	No Hit
AACGCCGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.1315843579094535	No Hit
AACGCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	32	0.1315843579094535	No Hit
AACGCCGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.12747234672478308	No Hit
AACGCCGGGCTGGAAACGCAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.12747234672478308	No Hit
AACGCCGGGATTGACTCTGAGCTCCCTCTGGAACCACATTCCGAAAGCTGTGCACT	28	0.11513631317077182	No Hit
AACGCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG	28	0.11513631317077182	No Hit
AACGCCGGGCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.10691229080143098	No Hit
AACGCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	25	0.10280027961676057	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGAC	15	0.008116743	50.331955	50
CCTCCAC	15	0.008116743	50.331955	50
ACCCTCC	15	0.008116743	50.331955	48
CGAGCGA	15	0.008116743	50.331955	49
CCGAGCG	15	0.008116743	50.331955	48
ATAAGCC	20	3.9704755E-4	50.331947	48
TAAGCCC	20	3.9704755E-4	50.331947	49
AAGCCCT	20	3.9704755E-4	50.331947	50
CAAGATA	15	0.008318894	50.02062	41
TGTTGGA	15	0.008318894	50.02062	33
TTGTTGG	15	0.008318894	50.02062	32
AACGTAT	15	0.008318894	50.02062	28
CAGTTAA	15	0.008318894	50.02062	28
CACACCT	15	0.008318894	50.02062	21
ACATGAT	15	0.008318894	50.02062	34
AGGATTT	20	4.0944296E-4	50.02062	41
AATTTCC	20	4.0944296E-4	50.02062	31
TCAGGGC	15	0.008318894	50.02062	26
CAACTCA	15	0.008318894	50.02062	20
TAAAAAT	20	4.0944296E-4	50.02062	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content