##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526827_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24319 Sequences flagged as poor quality 0 Sequence length 56 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.65376865825075 39.0 38.0 40.0 36.0 40.0 2 38.66462436777828 39.0 39.0 40.0 37.0 40.0 3 38.38558328878655 39.0 38.0 40.0 35.0 40.0 4 38.12504626012583 39.0 38.0 40.0 35.0 40.0 5 38.369793165837415 39.0 38.0 40.0 35.0 40.0 6 38.61482791233192 39.0 39.0 40.0 37.0 40.0 7 38.65899091245528 39.0 38.0 40.0 37.0 40.0 8 38.65516674205354 39.0 38.0 40.0 37.0 40.0 9 38.55697191496361 39.0 38.0 40.0 36.0 40.0 10 37.55989144290472 39.0 38.0 40.0 33.0 40.0 11 37.63674493194622 39.0 38.0 40.0 34.0 40.0 12 37.48443603766602 39.0 37.0 40.0 33.0 40.0 13 37.11147662321642 39.0 36.0 40.0 33.0 40.0 14 37.40930959332209 39.0 37.0 40.0 33.0 40.0 15 37.36798388091616 39.0 37.0 40.0 33.0 40.0 16 37.21230313746453 39.0 36.0 40.0 33.0 40.0 17 37.19770549775895 39.0 37.0 40.0 33.0 40.0 18 37.050248776676675 39.0 36.0 40.0 33.0 40.0 19 36.86327562810971 39.0 36.0 40.0 32.0 40.0 20 36.974341050207656 39.0 36.0 40.0 33.0 40.0 21 36.83954932357416 38.0 36.0 40.0 33.0 40.0 22 36.634031004564335 38.0 36.0 40.0 32.0 40.0 23 36.422838110119656 38.0 35.0 40.0 31.0 40.0 24 36.375591101607796 38.0 35.0 40.0 31.0 40.0 25 36.32785065175377 38.0 35.0 40.0 31.0 40.0 26 36.09375385501048 38.0 35.0 40.0 30.0 40.0 27 36.055676631440434 38.0 35.0 40.0 30.0 40.0 28 35.84732102471319 38.0 35.0 40.0 30.0 40.0 29 35.933220938360954 38.0 35.0 40.0 30.0 40.0 30 35.73341831489782 38.0 35.0 40.0 29.0 40.0 31 35.66548789012706 39.0 35.0 40.0 29.0 40.0 32 35.47370368847403 39.0 35.0 40.0 29.0 40.0 33 35.479542744356266 39.0 35.0 40.0 29.0 40.0 34 35.573790040708914 39.0 35.0 40.0 29.0 40.0 35 35.335252271886176 39.0 35.0 40.0 29.0 40.0 36 35.461943336485874 39.0 35.0 40.0 29.0 40.0 37 35.34545005962416 39.0 35.0 40.0 29.0 40.0 38 35.067889304658905 39.0 35.0 40.0 27.0 40.0 39 35.02495990789095 39.0 35.0 40.0 27.0 40.0 40 34.929150047288125 39.0 35.0 40.0 27.0 40.0 41 34.51836012993955 39.0 35.0 40.0 25.0 40.0 42 34.39882396480118 39.0 35.0 40.0 24.0 40.0 43 34.300012336033554 39.0 35.0 40.0 23.0 40.0 44 33.94839425963239 39.0 34.0 39.0 21.0 40.0 45 33.84781446605535 39.0 34.0 39.0 19.0 40.0 46 33.33882972161684 39.0 33.0 39.0 7.0 40.0 47 33.17924256753978 39.0 33.0 39.0 4.0 40.0 48 32.9950655865784 38.0 33.0 39.0 4.0 40.0 49 32.684896582918704 38.0 33.0 39.0 4.0 40.0 50 32.3742752580287 38.0 32.0 39.0 4.0 40.0 51 31.76047534849295 38.0 32.0 39.0 4.0 40.0 52 31.575270364735392 37.0 32.0 39.0 4.0 40.0 53 31.145071754595172 37.0 31.0 39.0 4.0 40.0 54 30.55471030881204 37.0 31.0 39.0 4.0 40.0 55 29.97294296640487 36.0 30.0 39.0 4.0 40.0 56 17.612196225173733 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5315113085438554 1 2 -0.40263315644731534 1 3 -0.44461244548956813 1 4 -0.6347704163419792 1 5 -0.9023758675641744 1 6 -0.5504896107380262 1 7 -0.9210771436431813 1 8 -0.9465665139642354 1 9 -0.9350957008792022 1 10 0.36462814992416526 1 11 -0.20535022141219628 1 12 -0.6714992928800925 1 13 0.43517020512734916 1 14 0.039407853274425975 1 15 -0.022827592610966008 1 16 0.7259500280134574 1 17 0.9386810402306764 1 18 -0.5951043926614545 1 19 -0.2280304565858131 1 20 0.3927418938366074 1 21 0.5685529986860303 1 22 0.6457808334534576 1 23 0.7039343367943687 1 24 0.6316663148346962 1 25 1.2767004803174729 1 26 0.9503517811538558 1 27 -0.3331387149109659 1 28 2.255126515596082 1 29 0.16477281844609593 1 30 0.26277209601409623 1 31 0.41836181305860265 1 32 0.37650854683794677 1 33 -0.8010075367944012 1 34 -0.15925754314891805 1 35 -0.4085598809778688 1 36 -1.0771106777893138 1 37 -0.11584442611393797 1 38 -0.3098185140645384 1 39 -0.14391682527704575 1 40 0.03575944348429516 1 41 0.6183055931006081 1 42 1.0454437995996955 1 43 0.6393118896024887 1 44 1.136912072943403 1 45 0.9422841071460795 1 46 1.6123484273356823 1 47 2.1708305570937014 1 48 1.593269929015392 1 49 2.2748011089391085 1 50 2.715958002198576 1 51 1.5554428744389988 1 52 2.3773487428786098 1 53 2.6234586858221753 1 54 1.1568544255486906 1 55 2.1774748247908313 1 56 2.4392416448744925 2 1 -0.18787494490749168 2 2 -0.7953604291745862 2 3 -0.8046124454895676 2 4 -0.6202249617965236 2 5 -0.7623758675641739 2 6 -0.5613987016471214 2 7 -0.5683498709159096 2 8 -0.42838469578241245 2 9 -0.28600479178829374 2 10 -1.320826395530382 2 11 -0.06535022141219571 2 12 0.6757734343926316 2 13 0.24971565967280185 2 14 -0.8205921467255735 2 15 -0.06646395624733259 2 16 0.0623136643770934 2 17 -0.1067735052238703 2 18 0.7158046982476378 2 19 0.6210604525050911 2 20 0.14910553020023798 2 21 1.18309845323148 2 22 -0.35785553018290983 2 23 0.48757070043073725 2 24 0.7298481330165174 2 25 -0.20511770150070419 2 26 -0.9914664006643221 2 27 0.08867946690721595 2 28 0.6623992428688084 2 29 0.886591000264275 2 30 0.9318630051050008 2 31 -0.472547277850488 2 32 0.7528721832015819 2 33 -0.21918935497622272 2 34 0.7861970023056273 2 35 0.26598557356758334 2 36 1.1647075040288684 2 37 0.9423373920678841 2 38 1.5229087586627372 2 39 0.11244681108658483 2 40 0.5103048980297515 2 41 0.23103286582788485 2 42 -0.3454652913093952 2 43 0.6629482532388522 2 44 0.7205484365797687 2 45 1.102284107146076 2 46 0.5105302455175007 2 47 1.1799214661846094 2 48 1.295088110833575 2 49 2.183892018030015 2 50 1.272321638562211 2 51 0.6772610562571764 2 52 0.10280328833315622 2 53 -0.15472313235964563 2 54 0.458672607366875 2 55 0.9556566429726523 2 56 0.2010598266926742 3 1 0.22101394398139718 3 2 0.21352845971429701 3 3 0.4072394063622866 3 4 -0.2913360729076402 3 5 -0.2542277194160292 3 6 0.32156426131584226 3 7 0.314613092047054 3 8 0.3345782671805466 3 9 0.2888100230265209 3 10 -0.23638195108593862 3 11 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3.60798847351834 3 53 2.249721312084798 3 54 0.6720059407002061 3 55 2.1067677540837657 3 56 0.34328204891489733 4 1 -0.38216065919320386 4 2 -0.8182175720317346 4 3 -0.44461244548956813 4 4 -0.3707011522727157 4 5 -0.830947296135605 4 6 -0.5937796540280686 4 7 -0.1721593947254334 4 8 -0.4379085053062255 4 9 -1.8593381251216314 4 10 -0.2522549669589509 4 11 -0.4196359356979116 4 12 -0.49184561322641684 4 13 0.08019185014899222 4 14 -0.07963976577319443 4 15 0.4706789008955212 4 16 0.43374223580565996 4 17 -0.011535409985775402 4 18 -0.33752863508569675 4 19 -0.4856062141615709 4 20 0.07672457781929154 4 21 -0.3102348801018522 4 22 -0.24166505399243476 4 23 0.18661831947835594 4 24 -0.7406280574596735 4 25 0.21392991754691337 4 26 0.1494859802880555 4 27 0.22963184785959356 4 28 -0.11283885236928626 4 29 -1.1534089997357242 4 30 -1.2090893758473769 4 31 -0.14873775404096534 4 32 0.2271578974872952 4 33 -0.952522688309557 4 34 0.100482716591344 4 35 0.3078903354723437 4 36 1.557088456409815 4 37 1.3213850111155026 4 38 1.0581468539008299 4 39 1.421018239658018 4 40 0.9903048980297484 4 41 0.39103286582788144 4 42 1.8354870896429816 4 43 2.5505673008579066 4 44 3.095786531817865 4 45 1.6565698214317948 4 46 2.763863578850838 4 47 2.579921466184608 4 48 1.2512785870240464 4 49 -1.0542032200652223 4 50 1.59041687665745 4 51 -1.3341675151713943 4 52 -2.291482425952559 4 53 -1.4804374180739295 4 54 -1.232755964061699 4 55 -1.8614862141702062 4 56 -0.3551306494977986 5 1 -0.19168446871701406 5 2 -0.19916995298411422 5 3 -0.20651720739432733 5 4 -0.4659392475108106 5 5 -0.2595187247070285 5 6 -0.49854155878997375 5 7 -0.505492728058762 5 8 -0.2950513624490796 5 9 -0.19267145845496003 5 10 -0.4903502050541917 5 11 -0.13392164998362688 5 12 -0.44422656560736584 5 13 0.318287088244233 5 14 0.2536935675601413 5 15 -0.7197972895806686 5 16 0.48136128342471096 5 17 1.1789407804904144 5 18 -0.14705244460950695 5 19 0.8001080715527138 5 20 0.6481531492478609 5 21 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0.5791380315809747 8 19 0.9727271191717577 8 20 0.23743886353357624 8 21 0.4814317865648192 8 22 0.573811136483755 8 23 0.9842373670973998 8 24 0.6998481330165163 8 25 0.5532156318326287 8 26 0.5602002660023402 8 27 0.47367946690721396 8 28 0.48239924286880864 8 29 1.38825766693094 8 30 1.2968630051050027 8 31 0.9107860554828449 8 32 1.6795388498682442 8 33 1.5058106450237787 8 34 1.927863668972293 8 35 2.337652240234249 8 36 1.5213741706955304 8 37 2.202337392067882 8 38 2.6295754253293993 8 39 0.6591134777532517 8 40 2.0319715646964127 8 41 3.1826995324945457 8 42 2.829534708690602 8 43 3.3362815865721913 8 44 3.4588817699131056 8 45 3.7339507738127438 8 46 4.055530245517502 8 47 4.829921466184608 8 48 4.691754777500236 8 49 3.0172253513633507 8 50 4.310654971895545 8 51 5.695594389590514 8 52 6.214469954999821 8 53 6.013610200973691 8 54 6.17200594070021 8 55 2.578989976305987 8 56 1.7877264933593437 9 1 0.2886467942229487 9 2 0.36811783169497403 9 3 0.16408320668434584 9 4 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2.702337392067882 115 38 2.3229087586627344 115 39 1.87244681108659 115 40 2.110304898029746 115 41 3.1910328658278786 115 42 2.8545347086906006 115 43 2.662948253238852 115 44 2.040548436579769 115 45 0.42228410714607634 115 46 1.4705302455175016 115 47 0.3799214661846051 115 48 0.8950881108335693 115 49 0.4638920180300161 115 50 0.5923216385622112 115 51 -4.362738943742823 115 52 -4.057196711666844 115 53 -5.354723132359645 115 54 -4.181327392633126 115 55 -6.004343357027349 115 56 -4.798940173307324 116 1 0.10485232781978482 116 2 0.27918502537086454 116 3 -0.2173397182168415 116 4 0.5470477654762007 116 5 0.6430786778903723 116 6 0.4040558438074271 116 7 0.3516501290840921 116 8 0.2807062133084912 116 9 0.519449753666251 116 10 -0.589917304621288 116 11 0.6582861422241706 116 12 0.32850070711990753 116 13 0.4806247505818959 116 14 0.13031694418351947 116 15 0.24989968011630026 116 16 -1.0013226992592763 116 17 -0.5158644143147768 116 18 -0.8678316653887279 116 19 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1.8324739760540396 117 39 2.5141859415213688 117 40 2.425087506725397 117 41 2.086685039740928 117 42 2.063230360864516 117 43 1.9377308619345044 117 44 0.9570701757102071 117 45 2.20315367236347 117 46 3.34357372377837 117 47 4.40600842270635 117 48 3.8620446325727045 117 49 4.05345723542132 117 50 4.221886855953514 117 51 4.932913230170225 117 52 3.335846766594024 117 53 3.457450780683832 117 54 3.903889998671225 117 55 3.3434827299291747 117 56 0.8167120006057189 118 1 -0.24479802183056876 118 2 -0.5984373522515085 118 3 -0.906150907028028 118 4 -0.21868650025806602 118 5 0.02070105551274537 118 6 0.32013975989134025 118 7 0.1978039752379388 118 8 0.1793076119098913 118 9 -0.679850945634449 118 10 -0.950057164761148 118 11 0.025419009357037226 118 12 -0.5980727194535191 118 13 0.12048489044203592 118 14 -0.4221306082640339 118 15 -0.04031011009348617 118 16 -0.40691710485367594 118 17 -0.8375427359931038 118 18 -0.004195301752361047 118 19 -0.6266318551872132 118 20 0.22141322250793394 118 21 0.6000215301545637 118 22 -0.25631706864444936 118 23 0.7214168542768888 118 24 0.4883096714780564 118 25 0.09488229849929297 118 26 0.1531489839510627 118 27 0.5025256207533673 118 28 0.17470693517650204 118 29 0.5388986925719692 118 30 1.0564783897203895 118 31 -0.5956242009274106 118 32 0.8205644908938865 118 33 1.496195260408392 118 34 0.0785046946133221 118 35 -1.9540144264324155 118 36 0.7970151963365595 118 37 0.31772200745249535 118 38 0.38598568173965475 118 39 -1.3537070350672593 118 40 -0.08661817889333179 118 41 -0.33973636494135206 118 42 -0.5839268297709381 118 43 -0.3816671313765312 118 44 0.3851638211951567 118 45 -0.057715892853920536 118 46 -0.4540851390978844 118 47 0.07992146618460794 118 48 -0.7633734276279682 118 49 -1.316107981969985 118 50 0.08309086933144272 118 51 -0.7627389437428214 118 52 -2.231042865512997 118 53 -1.8101077477442615 118 54 -3.0074812387869727 118 55 -1.5043433570273486 118 56 -1.5327863271534774 119 1 0.3821250550925086 119 2 0.2746395708254141 119 3 0.05538755451043187 119 4 0.21977503820347266 119 5 0.3476241324358256 119 6 0.35860129835288035 119 7 0.2516501290840907 119 8 0.3716153042175847 119 9 0.4239952082117071 119 10 0.7691736044696214 119 11 -0.8553502214121949 119 12 -1.3942265656073687 119 13 -1.6102843403271976 119 14 -1.0605921467255754 119 15 -0.43646395624733003 119 16 -1.3876863356229094 119 17 -1.4567735052238717 119 18 -1.204195301752364 119 19 -1.6189395474949038 119 20 -0.9208944697997623 119 21 -1.2769015467685136 119 22 -1.0178555301829064 119 23 -1.0324292995692659 119 24 -0.8001518669834837 119 25 -3.0551177015007056 119 26 -1.3814664006643227 119 27 -2.2513205330927875 119 28 -2.117600757131193 119 29 -2.4534089997357214 119 30 -1.1281369948949944 119 31 -1.1225472778504937 119 32 -0.8371278167984215 119 33 -1.0191893549762199 119 34 -1.0138029976943699 119 35 -1.604014426432414 119 36 -1.145292495971134 119 37 -1.6976626079321164 119 38 -1.287091241337265 119 39 -1.1575531889134112 119 40 -0.9596951019702544 119 41 -0.9089671341721157 119 42 -0.49546529130940087 119 43 -0.5470517467611415 119 44 -0.949451563420233 119 45 -1.407715892853922 119 46 -0.8694697544824947 119 47 -0.5200785338153935 119 48 -0.07491188916642955 119 49 0.5838920180300136 119 50 0.2523216385622078 119 51 -0.012738943742821363 119 52 0.2728032883331579 119 53 -0.4947231323596455 119 54 0.1886726073668754 119 55 -0.45434335702734785 119 56 0.22105982669267732 120 1 -0.2133294903620353 120 2 0.006457752643591164 120 3 0.10084209996497862 120 4 -0.2711340527056194 120 5 0.27944231425400545 120 6 0.22223766198924721 120 7 -0.7392589618250014 120 8 -0.5829301503278685 120 9 -0.6169138826973892 120 10 0.09190087719689188 120 11 -0.9780774941394696 120 12 -0.26240838378918596 120 13 -0.06482979487265084 120 14 -1.3696830558164805 120 15 -1.2501003198836997 120 16 -0.18314088107745619 120 17 -0.8340462324965969 120 18 -0.41328621084326755 120 19 -0.3189395474949066 120 20 -0.7890762879815796 120 21 0.5685529986860303 120 22 0.1457808334534576 120 23 0.4312070640671024 120 24 -0.4137882306198506 120 25 0.4130641166811131 120 26 0.04126087206294926 120 27 0.5304976487253938 120 28 0.5733083337779021 120 29 -0.6079544542811774 120 30 0.5354993687413696 120 31 1.009270903967689 120 32 0.5128721832015799 120 33 1.1080833722965053 120 34 1.2043788204874488 120 35 -0.49946897188696227 120 36 0.15016204948341283 120 37 0.38415557388606203 120 38 -0.08254578679181179 120 39 0.719719538359314 120 40 0.30848671621156853 120 41 0.48194195673697493 120 42 1.0454437995996955 120 43 0.7302209805115822 120 44 -1.1358151997838632 120 45 -0.01226134739937379 120 46 1.0668938818811355 120 47 1.5344669207300612 120 48 0.45690629265175176 120 49 2.1384374725754682 120 50 1.3523216385622092 120 51 2.3736246926208118 120 52 2.650076015605883 120 53 2.9416405040039884 120 54 1.838672607366874 120 55 0.35929300660901475 120 56 0.5756052812381292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 12.0 5 1.0 6 5.0 7 4.0 8 16.0 9 15.0 10 16.0 11 19.0 12 32.0 13 35.0 14 46.0 15 70.0 16 72.0 17 111.0 18 104.0 19 122.0 20 130.0 21 154.0 22 154.0 23 138.0 24 199.0 25 229.0 26 201.0 27 276.0 28 354.0 29 416.0 30 486.0 31 629.0 32 838.0 33 1040.0 34 1330.0 35 1687.0 36 2090.0 37 3832.0 38 5148.0 39 4308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.21049385254328 0.6044656441465521 0.0945762572474197 0.09046424606274929 8 99.08253106228915 0.7323294659754792 0.06994157821114129 0.11519789352423271 9 94.53102512438834 2.8948558740079773 1.1719231876310703 1.402195813972614 10 24.82832353304001 47.39915292569596 10.115547514289238 17.656976026974792 11 25.753526049590853 36.13635429088367 20.74920843784695 17.360911221678524 12 44.557753197088694 17.693984127636828 19.935030223282208 17.81323245199227 13 24.87355565607138 19.606069328508575 28.43866935318064 27.081705662239404 14 26.44845593980016 28.413997286072618 23.48780788683745 21.649738887289775 15 33.05234590238085 23.738640569102348 25.05859615938155 18.150417369135244 16 24.46235453760434 26.234631358197298 26.04959085488713 23.25342324931124 17 28.05214030182162 26.436119906246148 18.561618487602285 26.95012130432995 18 25.967350631193714 28.076812368929644 22.00337184917143 23.95246515070521 19 21.970475759694068 27.472346724783094 26.04959085488713 24.507586660635717 20 22.838110119659525 28.858094494017024 22.542045314363254 25.761750071960194 21 23.500143920391466 23.775648669764383 26.353879682552737 26.37032772729142 22 25.478021300217936 24.408898392203625 23.746864591471688 26.36621571610675 23 26.06192688844114 30.35897857642173 23.923681072412517 19.65541346272462 24 22.551196644460894 31.174438687392055 24.179620034542314 22.094744633604737 25 23.778582003619018 22.536601414706368 28.717716729725286 24.967099851949335 26 23.039901275195394 21.986836692719045 31.999177293294938 22.97408473879062 27 18.40134935000823 20.770939608359388 36.378969886457135 24.448741155175252 28 16.789432533640593 26.118266737994322 39.18357269248179 17.908728035883296 29 19.105657580499052 22.177386148398252 40.45952400559993 18.25743226550276 30 14.540059347181009 24.583745466534783 45.59841740850643 15.277777777777779 31 15.876288659793813 20.65567010309278 48.58144329896908 14.88659793814433 32 11.490081247164598 20.196312945931457 54.67068090897843 13.642924897925516 33 13.254950495049506 20.32178217821782 52.12046204620462 14.302805280528052 34 12.600049509035399 21.763346810792967 54.64147206865253 10.995131611519101 35 11.053761664877364 23.573375175489307 54.29432653398298 11.078536625650342 36 11.860417096840802 20.14866818087962 54.98657856700392 13.004336155275656 37 10.959639773619202 19.729830214400792 56.851323997190896 12.45920601478911 38 10.475206611570249 19.64876033057851 58.39256198347107 11.483471074380166 39 10.132286068623399 21.11616370400992 58.152128978916906 10.599421248449772 40 10.98605536475359 19.53076509289527 58.248851739976004 11.23432780237514 41 9.52814569536424 22.350993377483444 57.773178807947026 10.347682119205299 42 10.542654911213214 19.363384246036674 58.42543151620514 11.668529326544972 43 12.41356465570784 19.576829116806756 58.24603536085462 9.763570866630781 44 10.69328253126812 19.66371241613518 59.421850410005796 10.221154642590905 45 12.021541010770505 21.32974316487158 57.05882352941176 9.589892294946148 46 10.923255428476711 21.137908171722195 57.04044422343776 10.898392176363334 47 9.722222222222223 22.04809286898839 57.669983416252066 10.559701492537313 48 11.514372226139615 21.606039238458667 57.0990086689618 9.780579866439918 49 9.796442933543386 24.0703121761121 55.21744537954479 10.915799510799717 50 9.769352028540613 23.724383970795653 57.24301003899445 9.263253961669292 51 11.225378395189715 23.491602736885756 56.05639643375493 9.226622434169604 52 11.39786730841044 23.326832911497448 54.10563877017551 11.1696610099166 53 11.648269565939081 23.599468835588016 54.74313221014192 10.009129388330981 54 12.193187510435799 23.756052763399566 53.573217565536815 10.477542160627817 55 11.344014610659139 24.377386684376557 52.92213182799269 11.35646687697161 56 11.46438651834634 25.26564834799934 51.805578615307994 11.46438651834634 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 1.5 9 1.0 10 1.5 11 2.0 12 28.0 13 68.5 14 83.0 15 115.0 16 147.0 17 161.5 18 176.0 19 306.0 20 436.0 21 511.0 22 659.0 23 732.0 24 871.5 25 1011.0 26 1220.0 27 1429.0 28 1651.5 29 1980.5 30 2087.0 31 1946.5 32 1806.0 33 1830.5 34 1855.0 35 1418.0 36 981.0 37 1008.0 38 738.5 39 442.0 40 382.0 41 322.0 42 325.5 43 329.0 44 339.0 45 349.0 46 409.5 47 491.0 48 512.0 49 453.0 50 394.0 51 468.5 52 543.0 53 677.0 54 895.0 55 979.0 56 919.0 57 859.0 58 814.5 59 770.0 60 750.0 61 730.0 62 707.0 63 584.0 64 484.0 65 471.0 66 458.0 67 358.0 68 258.0 69 258.5 70 259.0 71 197.0 72 114.0 73 93.0 74 74.5 75 56.0 76 44.5 77 33.0 78 29.0 79 18.5 80 12.0 81 9.5 82 7.0 83 5.5 84 4.0 85 3.5 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.05345614540071549 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004112011184670422 25 0.012336033554011265 26 0.0370081006620338 27 0.045232123031374645 28 0.0740162013240676 29 0.13569636909412394 30 0.22616061515687322 31 0.28372877174225913 32 0.2960648052962704 33 0.32484888358896336 34 0.33307290595830424 35 0.4153131296517127 36 0.42764916320572394 37 0.46054525268308727 38 0.48932933097578024 39 0.5304494428224844 40 0.6250257000699042 41 0.6538097783625971 42 0.6579217895472677 43 0.690817879024631 44 0.7113779349479831 45 0.7360500020560056 46 0.7689460915333689 47 0.8182902257494141 48 0.8635223487807887 49 0.8141782145647437 50 0.8758583823348 51 0.8388502816727662 52 0.896418438258152 53 0.9087544718121633 54 1.4926600600353632 55 0.9334265389201858 56 0.9334265389201858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 24319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.419918582178546 #Duplication Level Percentage of deduplicated Percentage of total 1 71.79165359272042 37.6331263621037 2 13.727643551929713 14.392039146346477 3 5.624411672419203 8.844936058226079 4 2.9024160652651396 6.085776553312225 5 1.8120489488547225 4.749372918294338 6 1.153122058362096 3.6267938648793123 7 0.7609036711641042 2.7920555943912166 8 0.47066206463759025 1.9737653686418026 9 0.337307812990273 1.5913483284674532 >10 1.2864763100094132 10.843373493975903 >50 0.09413241292751805 3.338953081952383 >100 0.03922183871979918 4.128459229409104 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 487 2.0025494469344958 No Hit AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.6455857559932563 No Hit AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.6373617336239155 No Hit AACGCCGGGCTGGCTGCCCTATCCGGAACTTGATTAACTCTGTAGTCCAGGCTGGC 104 0.42764916320572394 No Hit AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.4153131296517127 No Hit AACGCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.3659689954356676 No Hit AACGCCGGGAGGTAGGCATCAGTGACAGACCAGAGCAGAGATTCCACTCAAATCTG 80 0.3289608947736338 No Hit AACGCCGGGAGCTTTAATGAGGAGCCTGTCGTGTTTCCCCATAAATTTATTGCCTG 76 0.3125128500349521 No Hit AACGCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 70 0.28784078292692955 No Hit AACGCCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69 0.28372877174225913 No Hit AACGCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69 0.28372877174225913 No Hit AACGCCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.2796167605575887 No Hit AACGCCGGGCTCGGTCACTGCTGGTCGCTTCCTGAGCCGCTGCTGGCTCTGTGTCT 65 0.26728072700357747 No Hit AACGCCGGGAGGAGTGAAGCTTCTAGTTAACACCAGCTTTACTTTTCTGTGCTTGA 64 0.263168715818907 No Hit AACGCCGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC 55 0.22616061515687322 No Hit AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.22616061515687322 No Hit AACGCCGGGGGAACTGTGTTAGAATATAAAAATTTCCCCAAGGATTTATAAGCCCT 52 0.21382458160286197 No Hit AACGCCGGGATCATTACACTGGCAACTCCGGGCAGCAATAATGGTCAGCAAGTGTG 47 0.19326452567950983 No Hit AACGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.19326452567950983 No Hit AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.1891525144948394 No Hit AACGCCGGGGGGCAAGCCTTCACACCTCAGTTAACATGATCAAGATAACCCTCCAC 45 0.185040503310169 No Hit AACGCCGGGACTGAAGCTTGGGACGTTTGTTCTTCGGTTCACGTGCTCCTCAGCAC 43 0.17681648094082816 No Hit AACGCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 41 0.16859245857148733 No Hit AACGCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.1644804473868169 No Hit AACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.1480324026481352 No Hit AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.1480324026481352 No Hit AACGCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 34 0.13980838027879436 No Hit AACGCCGGGAAGGGGTCCAGCTCCTAGAATGAGAACCAAAGGATTAGCTCTCTGAC 34 0.13980838027879436 No Hit AACGCCGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.1315843579094535 No Hit AACGCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 32 0.1315843579094535 No Hit AACGCCGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.12747234672478308 No Hit AACGCCGGGCTGGAAACGCAGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.12747234672478308 No Hit AACGCCGGGATTGACTCTGAGCTCCCTCTGGAACCACATTCCGAAAGCTGTGCACT 28 0.11513631317077182 No Hit AACGCCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG 28 0.11513631317077182 No Hit AACGCCGGGCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.10691229080143098 No Hit AACGCCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 25 0.10280027961676057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.01644804473868169 0.0 0.0 0.0 0.0 11 0.03289608947736338 0.0 0.0 0.0 0.0 12 0.03289608947736338 0.0 0.0 0.0 0.0 13 0.03289608947736338 0.0 0.0 0.0 0.0 14 0.04112011184670422 0.0 0.0 0.0 0.0 15 0.04112011184670422 0.0 0.0 0.0 0.0 16 0.04934413421604507 0.0 0.0 0.0 0.0 17 0.05345614540071549 0.0 0.0 0.0 0.0 18 0.057568156585385916 0.0 0.0 0.0 0.0 19 0.057568156585385916 0.0 0.0 0.0 0.0 20 0.06579217895472676 0.0 0.0 0.0 0.0 21 0.06990419013939718 0.0 0.0 0.0 0.0 22 0.07812821250873803 0.0 0.0 0.0 0.0 23 0.0945762572474197 0.0 0.0 0.0 0.0 24 0.10691229080143098 0.0 0.0 0.0 0.0 25 0.11513631317077183 0.0 0.0 0.0 0.0 26 0.11924832435544225 0.0 0.0 0.0 0.0 27 0.11924832435544225 0.0 0.0 0.0 0.0 28 0.13569636909412394 0.0 0.0 0.0 0.0 29 0.13980838027879436 0.0 0.0 0.0 0.0 30 0.15214441383280564 0.0 0.0 0.0 0.0 31 0.16448044738681689 0.0 0.0 0.0 0.0 32 0.16448044738681689 0.0 0.0 0.0 0.0 33 0.18092849212549858 0.0 0.0 0.0 0.0 34 0.18092849212549858 0.0 0.0 0.0 0.0 35 0.2014885480488507 0.0 0.0 0.0 0.0 36 0.21793659278753238 0.0 0.0 0.0 0.0 37 0.22616061515687322 0.0 0.0 0.0 0.0 38 0.23027262634154366 0.0 0.0 0.0 0.0 39 0.26316871581890705 0.0 0.0 0.0 0.0 40 0.2713927381882479 0.0 0.0 0.0 0.0 41 0.2755047493729183 0.0 0.0 0.0 0.0 42 0.28372877174225913 0.0 0.0 0.0 0.0 43 0.30017681648094086 0.0 0.0 0.0 0.0 44 0.3084008388502817 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGAC 15 0.008116743 50.331955 50 CCTCCAC 15 0.008116743 50.331955 50 ACCCTCC 15 0.008116743 50.331955 48 CGAGCGA 15 0.008116743 50.331955 49 CCGAGCG 15 0.008116743 50.331955 48 ATAAGCC 20 3.9704755E-4 50.331947 48 TAAGCCC 20 3.9704755E-4 50.331947 49 AAGCCCT 20 3.9704755E-4 50.331947 50 CAAGATA 15 0.008318894 50.02062 41 TGTTGGA 15 0.008318894 50.02062 33 TTGTTGG 15 0.008318894 50.02062 32 AACGTAT 15 0.008318894 50.02062 28 CAGTTAA 15 0.008318894 50.02062 28 CACACCT 15 0.008318894 50.02062 21 ACATGAT 15 0.008318894 50.02062 34 AGGATTT 20 4.0944296E-4 50.02062 41 AATTTCC 20 4.0944296E-4 50.02062 31 TCAGGGC 15 0.008318894 50.02062 26 CAACTCA 15 0.008318894 50.02062 20 TAAAAAT 20 4.0944296E-4 50.02062 27 >>END_MODULE