FastQCFastQC Report
Fri 17 Jun 2016
SRR1526826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185964
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT58123.1253360865543867No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.30489772213976896No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.22423694908692005No Hit
AAAACGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT2760.14841582241724205No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT2650.14250069906003313No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.14034974511195714No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.1371233141898432No Hit
AAAACGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT2450.13174592931965326No Hit
AAAACGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1980.1064722204297606No Hit
AAAACGGGGAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTT1910.10270805102062765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATGG201.9948561E-458.065150
CGGGATC407.075869E-1058.06515
TGATCGG150.00466029158.06509851
TTGATCG150.00466029158.06509850
CGGGTAT150.00466029158.0650985
ACGGGTA1400.058.0650984
AGGTACG202.0001916E-458.0337543
TACGCCA258.611292E-658.0337553
CTACGTA258.611292E-658.0337539
TACGTAC258.611292E-658.0337540
CCTTAAC150.0046702758.03374539
CGAAACA150.0046702758.03374543
CGTCTCA150.0046702758.03374541
TTATAAC150.0046702758.03374538
AGACACG150.0046702758.03374555
TACGTTT150.004675265458.0180835
AACGTAC202.008216E-457.98678229
CTTATAT150.004685268357.9867830
CTAAATG150.004685268357.9867829
TATCATG150.004725439557.86190813
Produced by FastQC (version 0.11.5)