FastQCFastQC Report
Fri 17 Jun 2016
SRR1526825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265373
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT141235.321943076349139No Hit
AAAACGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC12440.46877414054933997No Hit
AAAACGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG11340.4273230509509257No Hit
AAAACGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT11060.41677186450769294No Hit
AAAACGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT10170.3832341647417032No Hit
AAAACGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT10110.3809731962181533No Hit
AAAACGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC9910.3734366344729871No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8550.32218801460585667No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.2656638015171099No Hit
AAAACGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATT6950.26189552064452676No Hit
AAAACGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA6840.2577504116846853No Hit
AAAACGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG6520.24569191289241935No Hit
AAAACGGGGAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT6060.228357820878537No Hit
AAAACGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGG5750.2166761501735293No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4470.1684421550044654No Hit
AAAACGGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGC4350.16392021795736567No Hit
AAAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG4310.1624129056083324No Hit
AAAACGGGGAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTT4280.1612824213465575No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG4070.15336903151413295No Hit
AAAACGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT3780.1424410169836419No Hit
AAAACGGGGCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA3780.1424410169836419No Hit
AAAACGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC3580.13490445523847566No Hit
AAAACGGGGCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAG3530.1330203148021841No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3510.13226665862766745No Hit
AAAACGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG3380.1273678934933094No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.12548375305701787No Hit
AAAACGGGGAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG3280.1235996126207263No Hit
AAAACGGGGAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATC2880.10852648913039382No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.10626552060684395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACTA150.004645118558.11989654
TTAGGCT150.00466253558.0649942
TACCACG150.00466253558.0649945
CGACTAT150.00466253558.0649944
ATTGCGC150.00466253558.0649941
ATACACC150.00466253558.0649944
ACAATGC150.004666024458.05402438
TATCGTT202.0020512E-458.032136
TTCGTAT150.004673008358.03209736
TACGTTT150.004676503658.0211435
TGACCTA150.004680000758.01019333
CTACGTT150.004680000758.01019334
CGGTATG202.0114146E-457.97736428
TGATACC150.00469400857.96642726
CGGGACT258.700454E-657.9445725
CGGGATA202.0170493E-457.944575
CCACGGT150.004704533657.93364721
CGTCAGT150.004704533657.93364721
CCCTAGT150.00471156157.91181611
CAATGGT150.00471156157.91181613
Produced by FastQC (version 0.11.5)