FastQCFastQC Report
Fri 17 Jun 2016
SRR1526824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262535
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT131495.008475060468128No Hit
AAAACGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC11260.4288951949263908No Hit
AAAACGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG10920.41594454072790293No Hit
AAAACGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT10740.40908831203458584No Hit
AAAACGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT9660.36795093987468336No Hit
AAAACGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT9420.35880930161692726No Hit
AAAACGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC9080.34585864741843947No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7460.2841525891785857No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.2540613632468052No Hit
AAAACGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATT6490.24720513455348808No Hit
AAAACGGGGAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT5910.22511284209724416No Hit
AAAACGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA5880.22397013731502466No Hit
AAAACGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG5810.22130382615651248No Hit
AAAACGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGG5460.20797227036395147No Hit
AAAACGGGGAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTT4540.17292932370921973No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4440.16912030776848802No Hit
AAAACGGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGC3910.14893252328260995No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG3860.14702801531224408No Hit
AAAACGGGGCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA3740.14245719618336602No Hit
AAAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG3650.13902908183670748No Hit
AAAACGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT3580.1363627706781953No Hit
AAAACGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC3450.13141104995524408No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3420.13026834517302455No Hit
AAAACGGGGCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAG3170.12074580532119526No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.11807949416268307No Hit
AAAACGGGGAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG3010.11465137981602454No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.10931875749900014No Hit
AAAACGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2860.10893785590492695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG150.004531522358.48417345
CGTGTAA150.004573237658.34900326
AATAGGG150.00458371158.31530422
ATATATC201.9562419E-458.3040821
CAAGCGA201.999435E-458.0471450
GTTACGT150.00467168558.03601546
CGTAACT150.004682325858.00268637
TTGCGAC150.004700100557.9472135
CGTAACC202.0165417E-457.9472135
AACGTAC202.0318452E-457.85867329
TCTTACG202.0318452E-457.85867330
ACGTACA202.0318452E-457.85867330
TACGTAA202.0318452E-457.85867333
CGGTAGG150.004728644657.8586731
CCGTTAA150.004728644657.8586730
TAACGGG150.00473938257.82554227
ACGGGAT2050.057.8145034
CGGGATA202.0395315E-457.8145035
CCGATAT202.0549734E-457.7263719
ACCGATA202.0588483E-457.70438418
Produced by FastQC (version 0.11.5)