SRR1526823_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233117
Sequences flagged as poor quality	0
Sequence length	56
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	14738	6.322147247948455	No Hit
AAAACGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	1068	0.458139046058417	No Hit
AAAACGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	984	0.4221056379414629	No Hit
AAAACGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	928	0.39808336586349347	No Hit
AAAACGGGGAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT	824	0.3534705748615502	No Hit
AAAACGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	774	0.3320221176490775	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	707	0.30328118498436407	No Hit
AAAACGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	686	0.2942728329551256	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	659	0.2826906660603903	No Hit
AAAACGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	580	0.2488021036646834	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	575	0.24665725794343613	No Hit
AAAACGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	525	0.22520880073096344	No Hit
AAAACGGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA	510	0.21877426356722163	No Hit
AAAACGGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTC	481	0.20633415838398741	No Hit
AAAACGGGGAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAA	438	0.18788848518126092	No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	413	0.17716425657502455	No Hit
AAAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	367	0.15743167593954965	No Hit
AAAACGGGGAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTA	362	0.15528683021830242	No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA	336	0.1441336324678166	No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	331	0.1419887867465693	No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA	324	0.13898600273682313	Illumina Single End Adapter 1 (95% over 21bp)
AAAACGGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCA	323	0.13855703359257368	No Hit
AAAACGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	320	0.13727012615982534	No Hit
AAAACGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	312	0.13383837300582968	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	308	0.13212249642883186	No Hit
AAAACGGGGCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCA	307	0.13169352728458242	No Hit
AAAACGGGGCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT	283	0.12139826782259552	No Hit
AAAACGGGAAAGAAACCTGAGGAAAGCGTGGTCTTGAAGTATAAATATGTTTTTAT	279	0.1196823912455977	No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.11796651466859989	No Hit
AAAACGGGGAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAG	274	0.11753754552435043	No Hit
AAAACGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	254	0.10895816263936135	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	246	0.10552640948536572	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAATA	15	0.008280365	50.21583	45
CTAATAG	15	0.008280365	50.21583	46
CACTTAT	20	4.0890812E-4	50.21583	45
CGTTGAC	15	0.008301624	50.183273	42
CAATTTG	15	0.008301624	50.183273	41
ATATAGG	15	0.008301624	50.183273	42
CGTCAGT	15	0.008301624	50.183273	42
AGCAATT	15	0.00831582	50.16159	39
CACCTCG	20	4.1109518E-4	50.16159	39
AGTACGT	15	0.008322925	50.150753	38
TATCGTT	15	0.008330034	50.139927	36
ATACAAA	15	0.008330034	50.139927	36
TTATCGT	15	0.008344266	50.118282	35
AGATTGC	20	4.1682515E-4	50.02111	32
CGTACAC	15	0.008408533	50.02111	31
CTTATAT	15	0.008415697	50.010338	30
ACGTACA	15	0.008415697	50.010338	30
AACGTAC	15	0.008422865	49.99957	29
CGTCTTA	15	0.008430038	49.988808	27
CATAACC	15	0.008430038	49.988808	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content