FastQCFastQC Report
Fri 17 Jun 2016
SRR1526822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111452
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT90558.124573807558411No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT5870.5266841330797114No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT3890.3490291784804221No Hit
GCGAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT2960.26558518465348313No Hit
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.2575099594444245No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.22790080034454296No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT2290.20546961920826903No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAG1580.14176506478125112No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC1460.13099809783583963No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAGCCATGCAT1190.10677242220866383No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1180.10587517496321286No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAGCCATGCAT1120.10049169149050712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG150.00451437358.51769656
TTGCCAT201.9163197E-458.51769657
TGTCGAT150.00451437358.51769658
CCATGTA258.175786E-658.51769357
TGGGTAT303.6882375E-758.0959476
CGAATCG150.004645606558.0959472
ACGCCAT201.9905697E-458.06979454
CATGAGA201.9905697E-458.06979450
AACCCAC150.00465390258.0697954
GTTAGTA150.00465390258.0697955
TAAGTCA303.6997517E-758.0697953
CAGATTG150.00465390258.0697953
AGCGCTG150.00465390258.0697955
AGATTGC150.00465390258.0697954
AGCTATG303.6997517E-758.0697955
GGTTAGT150.00465390258.0697954
CATCAAT150.00465390258.0697953
ATTAAGT258.557148E-658.06978651
TACGCCA453.092282E-1158.06978653
TTAAGCT351.6079866E-858.06978652
Produced by FastQC (version 0.11.5)