FastQCFastQC Report
Fri 17 Jun 2016
SRR1526821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164494
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2469915.01513733023697No Hit
GCGAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT9180.5580750665677776No Hit
GCGAATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG6460.39271949128843603No Hit
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.2826850827385802No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.25958393619220155No Hit
GCGAATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG3520.21398956800855956No Hit
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2710.1647476503702263No Hit
GCGAATGGGTTACGCAGAGTCGACTTTTTAGTTATTTTTATTTTTTGTATTTTGTATTTTTCAC2660.16170802582465013No Hit
GCGAATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2450.1489416027332304No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2400.14590197818765427No Hit
GCGAATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG2260.13739102946004111No Hit
GCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAAC2230.13556725473269543No Hit
GCGAATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2120.12888008073242793No Hit
GCGAATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT2060.12523253127773656No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2050.12462460636862135No Hit
GCGAATGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT2030.1234087565503909No Hit
GCGAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA1910.11611365764100819No Hit
GCGAATGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA1720.10456308436781889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATGC150.004618108758.19553456
GACGCGT150.004618108758.19553458
TTAAGAC150.004623708358.17773452
TATTAGT303.6633537E-758.17773452
AACTGCC150.004623708358.17773454
TAAGACA150.004623708358.17773453
TAGTCAG303.6633537E-758.17773455
TAACAAC150.004634922458.14216244
TACGTTC150.004634922458.14216239
TTTACGC201.9811366E-458.1421644
CTACGTA406.9849193E-1058.1421639
CGTACTG406.9849193E-1058.1421642
ACGTACT406.9849193E-1058.1421641
GTACGTT150.00464615758.10663238
TCTACGT407.021299E-1058.1066338
GTACTAT201.9871347E-458.1066338
CTGTACG150.004657411558.07114836
TTAGTAC201.9931475E-458.07114435
AACTCTA407.057679E-1058.07114435
AAATTCC150.004663046458.0534234
Produced by FastQC (version 0.11.5)