FastQCFastQC Report
Fri 17 Jun 2016
SRR1526820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163389
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2318914.19250989968725No Hit
GCGAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT8640.5287993683785323No Hit
GCGAATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG5840.3574292027003042No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.27786448292112687No Hit
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.2307376873596142No Hit
GCGAATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG3220.19707569052996224No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2860.17504238351419005No Hit
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2590.1585174032523609No Hit
GCGAATGGGTTACGCAGAGTCGACTTTTTAGTTATTTTTATTTTTTGTATTTTGTATTTTTCAC2470.15117296758043686No Hit
GCGAATGGGTACCTGGTTGATCCTGNCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2420.14811278605046851No Hit
GCGAATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2370.14505260452050014No Hit
GCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAAC2150.13158780578863938No Hit
GCGAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA2150.13158780578863938No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2040.12485540642270901No Hit
GCGAATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1970.12057115228075328No Hit
GCGAATGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT1950.11934707966876595No Hit
GCGAATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG1910.11689893444479127No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCNCAAAGATTAAGCCATGCAT1800.11016653507886089No Hit
GCGAATGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA1710.10465820832491783No Hit
GCGAATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1680.1028220994069368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAT150.004526375358.4908545
ATTATCC150.004526375358.4908545
TCACAAA201.9233546E-458.49084545
AACCAAT201.9351902E-458.41834639
TTGTCCC150.00454866758.41834340
TTATACT150.00454866758.41834343
TACCTCT150.00454866758.41834339
TCACTAG150.00454866758.41834343
CTCAATG150.0045710458.3460226
AATTACG201.9470838E-458.3460226
ATAACAT150.0045710458.3460226
TTTTAGT303.5907033E-758.3460226
TGTTATC150.004582257558.3099324
ATCGTTG201.956042E-458.29189323
AATCGTT201.956042E-458.29189322
TTAATCG201.9590353E-458.2738820
TAATCGT201.9590353E-458.2738821
ACGCGAC201.9861397E-458.1122158
TCGTTGC258.537854E-658.11220658
CTAATTC150.00464431958.11220658
Produced by FastQC (version 0.11.5)