FastQCFastQC Report
Fri 17 Jun 2016
SRR1526819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151585
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2616317.259623313652405No Hit
GCGAATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC7550.49807038955041727No Hit
GCGAATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC5460.36019395058877857No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4710.31071675957383643No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA4660.30741828017284034Illumina Single End Adapter 1 (95% over 21bp)
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.26849622324108585No Hit
GCGAATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC3000.19790876405976843No Hit
GCGAATGGGTTACGCAGAGTCGACTTTTTAGTTATTTTTATTTTTTGTATTTTGTA2860.18867302173697925No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2580.17020153709140085No Hit
GCGAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA2480.16360457828940858No Hit
GCGAATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT2350.15502853184681864No Hit
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2100.1385361348418379No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2100.1385361348418379No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2090.13787643896163868No Hit
GCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT2030.13391826368044332No Hit
GCGAATGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC2000.13193917603984562No Hit
GCGAATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1950.1286406966388495No Hit
GCGAATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1890.12468252135765412No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG1890.12468252135765412No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1810.11940495431606031No Hit
GCGAATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1700.11214829963386878No Hit
GCGAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1650.10884982023287265No Hit
GCGAATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA1640.10819012435267343No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1590.10489164495167727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACA150.00797751250.68255250
ACGACTC203.902112E-450.6825550
TAGCGAT203.9150604E-450.64855649
AGGGTAT203.9150604E-450.64855649
ACTATCA150.00801994350.61461348
GTAGCGA203.9280427E-450.6146148
GAGGGTA203.9280427E-450.6146148
CAACGAT150.00823462450.2776346
CGATGCA150.00823462450.2776347
ACCATCG150.00825632550.2441844
CCACGAT150.00825632550.2441844
TTGTTGA150.00826719150.2274743
TTATTTG150.00826719150.2274743
GCCACGA150.00826719150.2274743
CCTCAAA204.079814E-450.22746743
GCCTCAA204.079814E-450.22746742
GTTTATT150.00827806850.21077341
TTCATGT204.0865166E-450.2107741
GATTAAG29800.050.1723250
AGACTCG150.00831076350.16074440
Produced by FastQC (version 0.11.5)