FastQCFastQC Report
Fri 17 Jun 2016
SRR1526818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47334
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4941.0436472725736259No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.8830861537161447No Hit
GTCCCGGGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTATATTCA740.15633582625596823No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.14365994845143026No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT610.12887142434613597No Hit
GTCCCGGGGTGGGGTGCATGACAGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACCCTAG540.1140829002408417No Hit
GTCCCGGGGGAGGAAGACTGGAACTTGATGTTTACTTGAATAAATGGTTCTAAATTTGGTTCTG500.10563231503781638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGT150.004504868858.49572457
GTATATT258.120767E-658.49572456
CCGAGTT150.00462046958.1231424
CCCGAGT150.00462046958.1231423
ACCGCTC150.004639948758.0615146
AACTTTC150.004639948758.0615144
GCCGACG201.9801725E-458.0615151
AATCTGA150.004639948758.0615155
GACGGGC150.004639948758.0615154
CAACTTT150.004639948758.0615143
ACTTTCG150.004639948758.0615145
AGTATAT258.488412E-658.06150455
TTATAGT258.488412E-658.06150451
TATAGTA258.488412E-658.06150452
ATAGTAT258.488412E-658.06150453
GTTATAG258.488412E-658.06150450
TAGTATA258.488412E-658.06150454
CCCTGAA150.004659489358.037
ACAACCA150.004659489358.038
ACCATCC150.004659489358.041