FastQCFastQC Report
Fri 17 Jun 2016
SRR1526817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61340
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11741.9139223997391586No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.986305836322139No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3260.5314639713074666No Hit
GTCCCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG3040.49559830453211606No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2600.42386697098141507No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1820.29670687968699055No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1700.27714378871861756No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT1570.25595044016954677No Hit
GTCCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1420.2314965764590805No Hit
GTCCCGGGGCTTGGATCTATTCTTGGTAAGGGGAGGCGGTGGTGTTTCCTTGTGTTGGATGGCA1260.20541245516791656No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.19400065210303227No Hit
GTCCCGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1100.1793283338767525No Hit
GTCCCGGGGGCTGGGGCAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTCTTGCTCCT1030.16791653081186828No Hit
GTCCCGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1000.16302575806977504No Hit
GTCCCGGGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTATATTCA960.156504727746984No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT930.15161395500489078No Hit
GTCCCGGGGTGGGGTGCATGACAGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACCCTAG920.149983697424193No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.143462667101402No Hit
GTCCCGGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCCTCCCACTG860.14020215194000654No Hit
GTCCCGGGGAGAAGAGATTACTGTAATTTCTTTCAGAAGCCTGTTCCCAGAACGTGGGCATCAA860.14020215194000654No Hit
GTCCCGGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATGCAAAACAG820.13368112161721554No Hit
GTCCCGGGGAGGGAAGATGGACGCAGCCACTTTGACATATGATACTCTCCGGTTTGCTGAATTT810.13205086403651778No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT810.13205086403651778No Hit
GTCCCGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG790.12879034887512225No Hit
GTCCCGGGGAGGAGGCATTTGAGCAATGACTTGAAGATGGGAAGGAAAGACTGTGAAGAAATAA750.12226931855233127No Hit
GTCCCGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG740.12063906097163353No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA730.11900880339093577No Hit
GTCCCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG690.11248777306814477No Hit
GTCCCGGGGGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAG640.104336485164656No Hit
GTCCCGAGGGTCGAGGCCTCTGTGGTTGTGCCGCGGGTTTGCTGCTGTGCTGAGTCCTTCTGGG630.10270622758395828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA258.462439E-658.1261356
TGATCAC150.004626089758.12612553
TGTGGGC150.004626089758.12612557
ATCACCC150.004626089758.12612555
GTCAGTC150.004626089758.12612558
ATCTTTA150.004626089758.12612554
TCTTTAA150.004626089758.12612555
GAACCTC150.004626089758.12612554
AAGATGC150.004626089758.12612554
CACCCTA150.004626089758.12612557
TAGTCAG150.004626089758.12612556
CGAAACT201.9819706E-458.07856446
AACCCTA201.9819706E-458.07856445
GCGAAAC201.9819706E-458.07856445
ACCCTAA201.9819706E-458.07856446
ACTACCG201.9819706E-458.07856444
TGTATCC150.004641125458.0785650
CATTCAT150.004641125458.0785650
ATCCGCG150.004641125458.0785647
ATTCATA150.004641125458.0785651
Produced by FastQC (version 0.11.5)