FastQCFastQC Report
Fri 17 Jun 2016
SRR1526816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62208
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11051.7762988683127572No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.8712705761316872No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2810.45171039094650206No Hit
GTCCCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG2690.4324202674897119No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2110.339184670781893No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1780.28613683127572015No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT1630.2620241769547325No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1510.24273405349794236No Hit
GTCCCGGGGCTTGGATCTATTCTTGGTAAGGGGAGGCGGTGGTGTTTCCTTGTGTTGGATGGCA1360.21862139917695472No Hit
GTCCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1210.1945087448559671No Hit
GTCCCGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1060.17039609053497942No Hit
GTCCCGGGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTATATTCA890.14306841563786007No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.1398533950617284No Hit
GTCCCGGGGGCTGGGGCAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTCTTGCTCCT860.13824588477366256No Hit
GTCCCGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT810.13020833333333331No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA800.1286008230452675No Hit
GTCCCGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG790.12699331275720166No Hit
GTCCCGGGGAAGCAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT770.12377829218106996No Hit
GTCCCGGGGATGATAGCTGGATATAGAGTTTTATTGTTTTATCGTCTGGTTGTATCTTTAATGC730.1173482510288066No Hit
GTCCCGGGGAGAAGAGATTACTGTAATTTCTTTCAGAAGCCTGTTCCCAGAACGTGGGCATCAA710.1141332304526749No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT700.11252572016460906No Hit
GTCCCGGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATGCAAAACAG700.11252572016460906No Hit
GTCCCGGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCCTCCCACTG680.10931069958847736No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10770318930041153No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC670.10770318930041153No Hit
GTCCCGGGGTGGGGTGCATGACAGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACCCTAG660.10609567901234568No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT660.10609567901234568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAC150.00451711958.4776244
TGTCGAT150.00451711958.4776244
TCCTACC150.004531705358.4300843
ACCTCAA150.004531705358.4300843
TACTCTC150.004531705358.4300843
TGATCCC150.00454632758.38261439
ACAGTGT150.00454632758.38261440
ATGATAC150.00454632758.38261439
ATACTCT150.00454632758.38261442
GGCATTA150.00454632758.38261440
AGTGTCG150.00454632758.38261442
TTGGTAA406.657501E-1058.3352323
TGATAGA150.004560983758.33522821
TAGAGAA150.004560983758.33522824
ATAGAGA150.004560983758.33522823
AAAACGC150.004560983758.33522822
CCTCCGA150.004560983758.33522823
AACGCTG150.004560983758.33522824
GATAGAG150.004560983758.33522822
TCCATTC150.004634796658.09943454
Produced by FastQC (version 0.11.5)