FastQCFastQC Report
Fri 17 Jun 2016
SRR1526815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57516
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12732.213297169483274No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6281.0918700883232493No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3360.5841852701856874No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3060.5320258710619654No Hit
GTCCCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2590.4503094791014674No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2350.4085819598024897No Hit
GTCCCGGGGCTTGGATCTATTCTTGGTAAGGGGAGGCGGTGGTGTTTCCTTGTGTT1760.30600180819250294No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA1480.25731970234369567No Hit
GTCCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1160.20168300994505878No Hit
GTCCCGGGGATCCATTTCATGGAAATTCCACACTGTTTTATACAAGATCCTTGAAA1150.19994436330760135No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.18429654357048475No Hit
GTCCCGGGGGCTGGGGCAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTC990.17212601710828293No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT980.17038737047082553No Hit
GTCCCGGGGATGATAGCTGGATATAGAGTTTTATTGTTTTATCGTCTGGTTGTATC950.1651714305584533No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC920.15995549064608108No Hit
GTCCCGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC910.15821684400862368No Hit
GTCCCGGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCC910.15821684400862368No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC880.15300090409625147No Hit
GTCCCGGGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAG830.14430767090896446No Hit
GTCCCGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC820.14256902427150706No Hit
GTCCCGAGGGTCGAGGCCTCTGTGGTTGTGCCGCGGGTTTGCTGCTGTGCTGAGTC760.13213714444676264No Hit
GTCCCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC730.12692120453439043No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC710.12344391125947561No Hit
GTCCCGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC680.1182279713471034No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC670.116489324709646No Hit
GTCCCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT640.1112733847972738No Hit
GTCCCGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT640.1112733847972738No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGGCACTGACTGCTCTTTC630.1095347381598164No Hit
GTCCCGGGGAGGGAAGATGGACGCAGCCACTTTGACATATGATACTCTCCGGTTTG620.107796091522359No Hit
GTCCCGGGGAGGAGGCATTTGAGCAATGACTTGAAGATGGGAAGGAAAGACTGTGA620.107796091522359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCAC150.00794684350.6908850
ATCTTCT204.0509974E-450.24583445
CCGCATC204.0509974E-450.24583441
TCCATCA150.00822909950.2458346
TGATGGT150.00822909950.2458346
TGCAATC150.00822909950.2458345
GCCGCAT204.0686066E-450.2017640
TTATACA204.0686066E-450.2017638
GCCTATG204.0686066E-450.2017639
CCTATGT204.0686066E-450.2017640
TATACAA204.0686066E-450.2017639
TTTTATA204.0862765E-450.1577636
GCCGTTA204.1217997E-450.06999629
CCGTTAA204.1217997E-450.06999630
CCACAAA150.008344064550.06999233
GGTCCTA150.008344064550.06999230
TATGCAG150.008344064550.06999230
CGTTAAA150.008344064550.06999231
GTTAAAG150.008344064550.06999232
CAGGTCC150.00837299150.02622628