FastQCFastQC Report
Fri 17 Jun 2016
SRR1526814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50927
Sequences flagged as poor quality0
Sequence length64
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7111.3961160091896245No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5871.1526302354350344No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.942525575824219No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.5792605101419679No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.45555402831503916No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.308284407092505No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.23366779900642096No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.22188622930861823No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.19832308991301276No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.1885051151648438No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.1885051151648438No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.16494197576923833No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11781569697802738No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.11585210202839358No Hit
TATGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG590.11585210202839358No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG570.11192491212912602No Hit
TATGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.10996131717949222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCA1050.058.6185357
CCCTGCA150.004469511658.61852657
CATGCCT150.004469511658.61852658
CATGCAT1000.058.61852658
GCCCTGC150.004469511658.61852656
TAAGCCC150.0046304958.09773353
TTAAGCC1300.058.09773352
AAGCCCT150.0046304958.09773354
TAAGCCA1150.058.0977353
AAGCCAT1150.058.0977354
TGTCTCA1450.058.0404441
AAACTGG150.0046486458.04043639
GGCAAAC150.0046486458.04043636
GCAAACT150.0046486458.04043637
CAAACTG150.0046486458.04043638
TTGTCTC1500.058.04043240
TCTCAAA1400.058.04043243
TAGGATG150.00466684257.9832535
TAGGAGT150.00466684257.9832535
AGGGTGA150.00466684257.9832536
Produced by FastQC (version 0.11.5)