FastQCFastQC Report
Fri 17 Jun 2016
SRR1526813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59582
Sequences flagged as poor quality0
Sequence length64
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT16722.806216642610184No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9731.6330435366385823No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7831.3141552817965156No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.6360981504481219No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.4850458192071431No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.3910577019905341No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2010.33735020643818603No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.2769292739417945No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.243362089221577No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.21818670068141383No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.18294115672518546No Hit
TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG980.16447920512906583No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.16280084589305494No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.14769561276895707No Hit
TATGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG870.1460172535329462No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC810.13594709811688094No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.13426873888087007No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.13259037964485917No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.1309120204088483No Hit
TATGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.1309120204088483No Hit
TATGTAGGGAAAGGGCAAGGCTAAGAAGCATCCTGAATCGGAGCCTGCAGAGGGTTATTAAGGC750.12587694270081567No Hit
TATGTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10741499110469604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC201.9814231E-458.0791355
GAGCGTG201.9814231E-458.0791342
GAGGAGC201.9814231E-458.0791339
GCGTGGC201.9814231E-458.0791344
CGTGGCC201.9814231E-458.0791345
TACGTAG201.9814231E-458.0791340
TTGATCA201.9814231E-458.0791350
TAGAGTT201.9814231E-458.0791344
TCTCCTC201.9814231E-458.0791353
GTACGTA201.9814231E-458.0791339
AGGAGCG258.500283E-658.0791340
ATCCAAA150.00464031158.07912441
CCGCCAA150.00464031158.07912453
CGCCAAA150.00464031158.07912454
TCATGGC150.00464031158.07912454
GCGGCAG201.9896946E-458.0302838
AGTACGT201.9896946E-458.0302838
GTGTCTC258.5856245E-657.98151425
AAACCCA201.9979935E-457.98151421
TGTCTCC258.5856245E-657.98151426
Produced by FastQC (version 0.11.5)