FastQCFastQC Report
Fri 17 Jun 2016
SRR1526812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58991
Sequences flagged as poor quality0
Sequence length64
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT16552.8055127053279314No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8911.5103998915088743No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6871.164584428133105No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.5746639317862047No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.4543065891407164No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.39158515705785624No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1660.28139885745283183No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.2610567713719042No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.2135919038497398No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.19494499160888948No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.186469122408503No Hit
TATGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG1050.1779932532081165No Hit
TATGTAGGGAAAGGGCAAGGCTAAGAAGCATCCTGAATCGGAGCCTGCAGAGGGTTATTAAGGC880.1491752979268024No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC860.14578495024664778No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.1373090810462613No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.135613907206184No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1339187333661067No Hit
TATGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.1237476903256429No Hit
TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG720.12205251648556559No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.101710430404638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCTT150.004542250758.39193745
CTGCCGC201.9374125E-458.34190821
TGCCGCA150.00455771358.341922
AGCAGTT150.00455771358.341925
CGTAAAC150.00455771358.341921
ATTTTGC150.00455771358.341921
GAAAGCA150.00455771358.341922
CCGTGCG150.004573214358.2919541
ATCCAAA150.004573214358.2919541
GCTGCCG406.693881E-1058.2919520
GCTCTGG150.004573214358.2919542
GTGCGAA150.004573214358.2919543
TCTTTCC150.004573214358.2919544
CGCGCTC201.9456468E-458.2919539
CCGAAAG150.004573214358.2919520
TTCGCCA201.9788627E-458.09300250
CTTCGCC201.9788627E-458.09300249
GCTTCGC201.9788627E-458.09300248
CGCCGCC150.004635612558.09300258
GGGCATC150.004698642557.895417
Produced by FastQC (version 0.11.5)