FastQCFastQC Report
Fri 17 Jun 2016
SRR1526811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50168
Sequences flagged as poor quality0
Sequence length56
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG18293.6457502790623506No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9801.9534364535161859No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA7981.5906553978631797No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.5361983734651571No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.5322117684579811No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.3488279381278903No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1690.3368681231063626No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.2830489555094881No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.2451762079413172No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.22922978791261361No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.22125657789826186No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.21328336788391006No Hit
TATGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC980.19534364535161858No Hit
TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG950.18936373784085472No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.17740392281932704No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.143517780258332No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA700.1395311752511561No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.13753787274756818No Hit
TATGTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.13155796523680433No Hit
TATGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1116249402009249No Hit
TATGTAGGGAAAGGGCAAGGCTAAGAAGCATCCTGAATCGGAGCCTGCAGAGGGTT560.1116249402009249No Hit
TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC550.10963163769733696No Hit
TATGTAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA540.107638335193749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT309.364958E-750.6761448
TTTGATC309.364958E-750.6761449
CCGTGCG204.017121E-450.3185541
CGTGCGA204.017121E-450.3185542
TGGTCCT150.00817599850.31854644
TAAAATT150.00817599850.31854646
TTGTATA150.00824144350.21730438
CCGGGCA150.00834033950.06619633
CTGTGCG150.00840675949.96596528
AACATTC150.00844011649.91627
CGAACAT150.00847357249.8661325
GAACATT150.00847357249.8661326
CGCTGTG150.00847357249.8661326
CGTAAAG150.00850712649.81636421
CTCATCG150.00850712649.81636421
ATCGACC150.00850712649.81636424
AGCCCGG150.00854077849.766719
AGGGGAT150.00854077849.76676
GGATGGA150.00854077849.76678
AGGGGAC402.7048372E-949.76676
Produced by FastQC (version 0.11.5)