##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526811_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50168 Sequences flagged as poor quality 0 Sequence length 56 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.32662254823792 39.0 38.0 40.0 35.0 40.0 2 37.53107558603094 39.0 38.0 40.0 33.0 40.0 3 37.92395550948812 39.0 38.0 40.0 35.0 40.0 4 37.65848748206028 39.0 38.0 40.0 33.0 40.0 5 37.34547918992186 39.0 38.0 40.0 33.0 40.0 6 37.82634348588742 39.0 38.0 40.0 34.0 40.0 7 38.12306649657152 39.0 38.0 40.0 35.0 40.0 8 38.16239435496731 39.0 38.0 40.0 35.0 40.0 9 38.07056290862701 39.0 38.0 40.0 35.0 40.0 10 37.10237601658428 39.0 37.0 40.0 32.0 40.0 11 37.285839579014514 39.0 37.0 40.0 33.0 40.0 12 37.19167596874502 39.0 37.0 40.0 33.0 40.0 13 36.92857997129644 39.0 36.0 40.0 32.0 40.0 14 37.12390368362303 39.0 36.0 40.0 33.0 40.0 15 37.12978392600861 39.0 37.0 40.0 33.0 40.0 16 36.91602216552384 39.0 36.0 40.0 32.0 40.0 17 36.98784085472811 39.0 36.0 40.0 33.0 40.0 18 36.81512119279222 39.0 36.0 40.0 32.0 40.0 19 36.63211608993781 39.0 36.0 40.0 31.0 40.0 20 36.75432546643279 39.0 36.0 40.0 32.0 40.0 21 36.69051985329293 39.0 36.0 40.0 32.0 40.0 22 36.580768617445386 39.0 36.0 40.0 31.0 40.0 23 36.411397703715515 38.0 36.0 40.0 31.0 40.0 24 36.28651730186573 38.0 35.0 40.0 31.0 40.0 25 36.21107080210493 38.0 35.0 40.0 31.0 40.0 26 35.97213363099984 38.0 35.0 40.0 30.0 40.0 27 36.036597033965876 38.0 35.0 40.0 30.0 40.0 28 35.6396109073513 38.0 35.0 40.0 29.0 40.0 29 35.71176845798119 38.0 35.0 40.0 29.0 40.0 30 35.43174932227715 38.0 35.0 40.0 29.0 40.0 31 35.21021368202838 38.0 35.0 40.0 28.0 40.0 32 34.938686014989635 38.0 34.0 40.0 27.0 40.0 33 35.02667038749801 38.0 35.0 40.0 27.0 40.0 34 35.067732419071916 38.0 35.0 40.0 28.0 40.0 35 34.78944745654601 38.0 34.0 40.0 27.0 40.0 36 34.79835751873704 39.0 35.0 40.0 26.0 40.0 37 34.664965715196935 39.0 35.0 40.0 25.0 40.0 38 34.5258332004465 39.0 35.0 40.0 24.0 40.0 39 34.46629325466433 39.0 35.0 40.0 24.0 40.0 40 34.22604050390687 39.0 34.0 40.0 23.0 40.0 41 34.085512677403926 39.0 34.0 40.0 20.0 40.0 42 33.951662414287995 39.0 34.0 40.0 19.0 40.0 43 33.78043772922979 39.0 34.0 40.0 17.0 40.0 44 33.60903364694626 39.0 33.0 40.0 15.0 40.0 45 33.424453835114015 38.0 33.0 40.0 10.0 40.0 46 33.01815898580769 38.0 33.0 40.0 4.0 40.0 47 32.753807207781854 38.0 33.0 39.0 4.0 40.0 48 32.58676845798119 38.0 32.0 40.0 4.0 40.0 49 32.30375936852177 38.0 32.0 39.0 4.0 40.0 50 32.1184420347632 38.0 32.0 39.0 4.0 40.0 51 31.322934938606284 37.0 32.0 39.0 4.0 40.0 52 31.16859352575347 37.0 32.0 39.0 4.0 40.0 53 30.79732100143518 37.0 31.0 39.0 4.0 40.0 54 30.219482538670068 36.0 30.0 39.0 4.0 40.0 55 29.767919789507257 36.0 29.0 39.0 4.0 40.0 56 17.35656195184181 21.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.13227534459228 1 2 -1.8570601455368134 1 3 -1.6010318369056407 1 4 -1.6696806772049584 1 5 -2.3188498741763865 1 6 -1.1542508018186055 1 7 -1.1317368973634103 1 8 -1.1399810272475648 1 9 -1.046365899118726 1 10 -1.254631515927663 1 11 -0.5485370996632426 1 12 -0.7482052008673747 1 13 -0.8660393859028375 1 14 -1.058198181294614 1 15 -1.6973618356240365 1 16 -0.540801348990037 1 17 -1.2146637116310686 1 18 -0.7854538437079768 1 19 -0.3745877735002239 1 20 -0.45802565818004837 1 21 -0.23334578735950373 1 22 -1.0180215445589411 1 23 -1.058014993426653 1 24 -1.0208427233427813 1 25 -0.8323773328048603 1 26 0.3830813666953148 1 27 0.3043766942407302 1 28 1.1906867609184246 1 29 0.33318671784002873 1 30 0.5595680692617009 1 31 0.34549087279253854 1 32 -0.10497651952538689 1 33 0.180631475555451 1 34 -0.2601477693342318 1 35 0.9024606725902942 1 36 0.17029314316739885 1 37 -0.2511427007741247 1 38 0.6267578591579479 1 39 -0.5884055698050332 1 40 -0.787231017988745 1 41 0.07221337314307874 1 42 -0.039437344310663036 1 43 0.4551160239032299 1 44 0.5680573149538333 1 45 -0.0678886271474255 1 46 -0.22811747380633562 1 47 1.0143001137970487 1 48 1.0926831942404718 1 49 0.5386005009963455 1 50 1.0374913537701822 1 51 -0.30364328585666556 1 52 0.04982416793490074 1 53 -0.8761743998502389 1 54 0.21555775530315202 1 55 0.19946767893911854 1 56 1.1485696326163435 2 1 -0.04526554067071231 2 2 0.39661632505141853 2 3 -0.16843379768995703 2 4 -0.14026891249907436 2 5 0.05369914543145882 2 6 -0.2522900175048761 2 7 0.3780670242052153 2 8 0.38943073745831924 2 9 0.46343802244989973 2 10 0.12772142524880792 2 11 -0.6723116094671653 2 12 -0.42345029890658736 2 13 -0.22388252315774082 2 14 -0.21751190678480725 2 15 0.08082443888577018 2 16 -1.25036017251945 2 17 -0.8506931233957715 2 18 -0.12246364762954443 2 19 -0.19444071467669488 2 20 -0.2791040895525967 2 21 -0.33751245402616803 2 22 0.24423335740184626 2 23 0.03512226147530839 2 24 -0.7022152723623876 2 25 -0.6179165484911309 2 26 1.2249931314011988 2 27 0.30070022365249116 2 28 0.27524558444783054 2 29 0.055000443330222026 2 30 -0.12915742093437643 2 31 -0.07730324485451945 2 32 0.4575234804746131 2 33 -0.845103818562194 2 34 0.5584796816461619 2 35 -0.6808726607430415 2 36 -0.07603038624436209 2 37 0.7488572992258753 2 38 0.8632774670010903 2 39 -1.0197781188246395 2 40 -1.1377212140671773 2 41 -0.14102192097457333 2 42 -0.3972804815655664 2 43 -0.21400162315558902 2 44 0.3327631973067753 2 45 0.03627803951924591 2 46 -0.1852253169435869 2 47 -0.015111650908835372 2 48 -0.4465324920340379 2 49 0.10967893236889381 2 50 0.3573442949466532 2 51 1.0174351455158828 2 52 -0.2308130869670606 2 53 0.3738256001497611 2 54 -0.14718734273606415 2 55 0.6896637573704893 2 56 -0.18598919091306954 3 1 -0.7781086779256157 3 2 -0.8987268122034777 3 3 -0.580198503572305 3 4 -0.4196806772049584 3 5 -1.5480165408430508 3 6 -0.2375841351519341 3 7 -0.6942368973634103 3 8 -0.4733143605809005 3 9 -0.5671992324520616 3 10 -1.3379648492609988 3 11 0.3056295670034217 3 12 -1.289871867534039 3 13 -0.9493727192361732 3 14 -1.558198181294614 3 15 -0.7598618356240365 3 16 -0.22830134899003696 3 17 -1.2354970449644043 3 18 -0.9937871770413125 3 19 -0.8120877735002239 3 20 -0.8330256581800484 3 21 -1.337512454026168 3 22 -0.3930215445589411 3 23 -1.5163483267599815 3 24 -0.27084272334278126 3 25 -0.01987733280486026 3 26 -1.7627519666380138 3 27 -0.7581233057592698 3 28 -0.5801465724149111 3 29 -0.06264661549330697 3 30 -0.4404319307382991 3 31 0.11632420612586714 3 32 1.020023480474613 3 33 1.2847981422221224 3 34 1.0315188973324325 3 35 0.9441273392569585 3 36 2.0036264765007346 3 37 1.9155239658925396 3 38 1.439257859157948 3 39 1.7032610968616382 3 40 1.4836023153445907 3 41 1.0930467064764073 3 42 1.6272293223560013 3 43 1.14261602390323 3 44 1.5055573149538333 3 45 1.7654447061859102 3 46 2.126049192860336 3 47 1.2643001137970487 3 48 1.134349860907136 3 49 0.7261005009963455 3 50 0.1624913537701822 3 51 0.8630233808099987 3 52 1.320657501268233 3 53 0.7904922668164289 3 54 0.965557755303152 3 55 0.09530101227245069 3 56 -0.7264303673836565 4 1 -0.4502101272009753 4 2 -0.37064710205855533 4 3 -0.5874448803838987 4 4 -0.756637198944091 4 5 -0.7101542220024726 4 6 -0.3426565989200512 4 7 -0.2404325495373243 4 8 -0.22693754898669738 4 9 -0.220278942596984 4 10 -0.6097039796957802 4 11 -0.9280660851704923 4 12 -0.5099805631862111 4 13 -0.5145901105405244 4 14 -0.5563865870917155 4 15 -0.04790531388490393 4 16 0.10684357854619009 4 17 -0.37498979858759185 4 18 -0.2609973219688442 4 19 0.11544845838383111 4 20 0.7503076751532873 4 21 -0.1925849177942851 4 22 -0.7770795155734334 4 23 -1.409464268788966 4 24 -0.70562533203843 4 25 -0.6213266081671733 4 26 -1.2102157347539588 4 27 -0.6566740303969567 4 28 -1.3355813550236064 4 29 -0.7293132821599713 4 30 -1.17775077131801 4 31 -0.8275163735842739 4 32 -0.28885333111959 4 33 -0.9887525824155645 4 34 -0.14058255194292713 4 35 -0.4634813563952136 4 36 0.5335177808485625 4 37 0.45900222676210234 4 38 0.19106945336084635 4 39 0.5275364591804745 4 40 -0.14411507595976047 4 41 0.5269235180706104 4 42 -0.7024808225715304 4 43 0.06562327028004233 4 44 -0.27976877200269 4 45 0.3551186192293869 4 46 -0.614892836125172 4 47 -0.0074390166377327205 4 48 -0.23340376228127013 4 49 -1.8690081946558301 4 50 -1.3583419795631535 4 51 -2.3742954597697086 4 52 -2.510864237862201 4 53 -2.4087830955024145 4 54 -3.690239346146125 4 55 -3.9019815964232016 4 56 -2.1186405123111935 5 1 -0.5238569772453445 5 2 0.5982969973203325 5 3 -0.21965428588523395 5 4 0.3507274860603502 5 5 0.7627827788848407 5 6 0.505613143759625 5 7 0.5009161638610777 5 8 0.4722638707116218 5 9 0.32098103965678604 5 10 0.3610147425757333 5 11 -0.8091663513639276 5 12 -1.2885963573299577 5 13 0.0914436072944369 5 14 -0.7733342357163835 5 15 -0.012412856032199215 5 16 -0.691736723139698 5 17 -0.04757187489637715 5 18 -1.337749762075326 5 19 -0.11480886193560025 5 20 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0.15384873455974457 6 5 0.34781679249027775 6 6 0.0614354726912012 6 7 0.064341534009138 6 8 0.25217583549753186 6 9 0.03206547343029342 6 10 -0.048749162986489125 6 11 0.11200211602302801 6 12 0.12556930893654794 6 13 0.12905865331284616 6 14 0.21386064223479906 6 15 -0.3113324238593336 6 16 -0.8582033097743533 6 17 -0.8114774371212619 6 18 -0.8087381574334671 6 19 -0.9983622833041466 6 20 -1.2791040895525967 6 21 -0.1414340226536197 6 22 -0.6577274269118831 6 23 -1.4158581306815492 6 24 -1.1531956645192523 6 25 -0.16693615633426617 6 26 0.10734607257766982 6 27 -0.1894958547788832 6 28 0.5693632315066566 6 29 -0.4548034782384036 6 30 0.06692101043817189 6 31 0.4128928335768478 6 32 0.9673274020432387 6 33 1.2529353971240837 6 34 1.0486757600775363 6 35 1.181872437296171 6 36 1.2180872608144568 6 37 1.1606220051082232 6 38 1.1770029571971676 6 39 1.0194375674498701 6 40 1.2740434918151777 6 41 1.7805467064764073 6 42 2.681150890983453 6 43 2.609527788609114 6 44 2.313155354169517 6 45 1.6637290199114005 6 46 2.873598212468174 6 47 2.710378545169597 6 48 2.9848400569855684 6 49 3.3841887362904615 6 50 2.180873706711356 6 51 2.1154743612021605 6 52 2.1613437757780396 6 53 1.903237364855645 6 54 1.6175185396168779 6 55 2.513193169135196 6 56 1.8924421816359498 7 1 0.21083690030567936 7 2 -0.8506825945164067 7 3 -0.668633877721966 7 4 -0.44519088128659234 7 5 -0.48211518029883393 7 6 -0.6168358358322052 7 7 -0.4378593463430036 7 8 -0.3644708231659308 7 9 -0.25044753177179047 7 10 -0.6798015839548768 7 11 0.15001732210546237 7 12 -0.594718806309551 7 13 -0.07182169882801048 7 14 -0.4876199500020917 7 15 -0.4818006111342399 7 16 -0.6101040700784779 7 17 -0.31287799734535326 7 18 0.009189013434877324 7 19 -0.11480886193560025 7 20 -0.34152905954059065 7 21 -0.038192726135015675 7 22 -0.6005045377562155 7 23 0.01511425827403201 7 24 0.17813686849395083 7 25 0.1399866127733702 7 26 -0.6241465244611462 7 27 -0.45880357786811743 7 28 0.13158812146264154 7 29 -1.0762520576701746 7 30 0.6199422189215653 7 31 -0.09370980747956992 7 32 0.06296565734535875 7 33 -0.04598417070305061 7 34 0.030668557196378288 7 35 0.5640252984406331 7 36 -0.11244495207069605 7 37 -0.21712909533194846 7 38 0.5659585394300564 7 39 0.9081930696507499 7 40 0.41089823371194 7 41 0.5628596316464751 7 42 0.6391340842607676 7 43 0.9236534388692164 7 44 1.0170369067905654 7 45 0.8866181755736662 7 46 2.4126138187106747 7 47 2.5755246035929673 7 48 2.1437036024037397 7 49 2.1789066234453216 7 50 3.2436988367633752 7 51 3.4948261018984397 7 52 3.578310562492721 7 53 2.7207643756599644 7 54 1.946850272309952 7 55 3.247086726558166 7 56 2.3862397006435536 8 1 0.07152900323380607 8 2 -0.2184731890150715 8 3 -0.02222748907955463 8 4 0.48249323583851833 8 5 0.7898457779975274 8 6 0.04864774890603485 8 7 0.47695875481050365 8 8 0.6208885379698259 8 9 0.5840688834899694 8 10 1.0207308029129152 8 11 0.5936730452642891 8 12 0.772628132465961 8 13 1.3984533677203501 8 14 0.48709167377785434 8 15 1.0390512078542216 8 16 0.824234882894018 8 17 1.059792810108064 8 18 0.9563939823789767 8 19 0.8545788931664404 8 20 1.2068294142837246 8 21 1.5248063865535428 8 22 1.1359639626874412 8 23 1.2209705138197293 8 24 1.7726355375267815 8 25 2.074325565745866 8 26 1.6376103522025645 8 27 1.7563694478639178 8 28 2.468766471063347 8 29 2.009817152622638 8 30 1.5179014025950366 8 31 2.607266235111375 8 32 2.885059712358668 8 33 1.815595243671396 8 34 1.6202870132744636 8 35 2.406083860996091 8 36 2.4248221286746485 8 37 2.611176139805586 8 38 2.951939018578237 8 39 2.6362321113543885 8 40 3.2471892718663327 8 41 2.5921409093749617 8 42 2.0583887426458602 8 43 1.9786667485409168 8 44 2.3724051410407867 8 45 3.072509923577215 8 46 3.2981506421356954 8 47 2.7316914181448766 8 48 3.2883353681535183 8 49 3.044035283605041 8 50 3.48948410739337 8 51 4.777878453273772 8 52 5.184787936050842 8 53 5.11295603493237 8 54 4.8967171755930075 8 55 3.9023662296637553 8 56 2.7074464442105466 9 1 0.46453838089791333 9 2 0.7495575015220126 9 3 0.831566202310043 9 4 0.8009075580891576 9 5 0.8772285571961618 9 6 0.8457491981813945 9 7 0.6721846712640414 9 8 0.5855091688308676 9 9 0.5418693949989191 9 10 1.4022312291703756 9 11 1.1512178022975377 9 12 1.4981183285443933 9 13 1.4035684572344138 9 14 1.449154759881857 9 15 1.3945499290818475 9 16 1.4751300235589824 9 17 1.4434245236630474 9 18 1.2893010582528106 9 19 1.9428141872840854 9 20 1.0738370869179903 9 21 1.9958208793071606 9 22 2.106978455441059 9 23 2.015514418338057 9 24 1.8664121786180061 9 25 2.421299137783379 9 26 1.3426401902247278 9 27 1.3007002236524912 9 28 1.5105397020948956 9 29 1.525588678624345 9 30 2.145352382987191 9 31 1.4325006767141062 9 32 1.398699951062845 9 33 1.4686216716338834 9 34 -0.12779482815776078 9 35 0.6916763588648038 9 36 0.3749500059125026 9 37 0.6116023972650879 9 38 0.5887676630795156 9 39 0.4900258027439861 9 40 1.0191415310308685 9 41 0.6628996476528783 9 42 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0.5394119547033611 120 42 0.6999243578169967 120 43 1.2645131870238018 120 44 1.248908378783618 120 45 1.2561361955476116 120 46 2.359205221229125 120 47 1.8547256457119445 120 48 2.3799172367936663 120 49 2.4880685861027274 120 50 1.971445254479402 120 51 2.2894418205263136 120 52 2.1584234587150384 120 53 2.5014851746178444 120 54 1.963784705657762 120 55 2.0647159058894715 120 56 2.1135519021198874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 153.0 5 8.0 6 22.0 7 23.0 8 88.0 9 84.0 10 83.0 11 117.0 12 88.0 13 105.0 14 128.0 15 143.0 16 159.0 17 209.0 18 261.0 19 254.0 20 298.0 21 344.0 22 359.0 23 385.0 24 463.0 25 538.0 26 552.0 27 630.0 28 746.0 29 879.0 30 1095.0 31 1400.0 32 1755.0 33 2012.0 34 2605.0 35 3156.0 36 4142.0 37 7015.0 38 9604.0 39 10265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.34819008132673 0.4106203157391165 0.19135704034444267 0.04983256258969861 8 99.04894826039278 0.7496760043864021 0.09171568138769813 0.10966005383311733 9 96.24860468824748 2.0949609312709296 0.5999840535799713 1.0564503269016106 10 32.26558762557806 40.14112581725403 12.874740870674534 14.718545686493382 11 21.71304417158348 40.872667836070804 20.184181151331526 17.23010684101419 12 45.6506139371711 20.08252272364854 17.355684898740233 16.91117844044012 13 24.290384308722693 18.91245415404242 27.663052144793493 29.1341093924414 14 28.970658587147184 30.019135704034444 24.264471376176047 16.74573433264232 15 38.75777387976399 22.600063785680117 22.833280178599903 15.808882155955988 16 27.671025354807842 25.47440599585393 25.63387019614097 21.220698453197258 17 28.225163450805297 24.549513634189125 23.393398182108115 23.831924732897463 18 25.528225163450806 29.698214000956785 25.428560038271407 19.345000797321003 19 26.070403444426727 26.413251475043854 27.99992026789986 19.516424812629566 20 22.163530537394355 32.159942592887894 21.009408387816936 24.66711848190081 21 21.675171424015307 26.801945463243506 30.69287195024717 20.83001116249402 22 21.820682506777228 28.20323712326583 23.032610428958698 26.943469940998245 23 26.4052782650295 30.47161537234891 23.564822197416678 19.558284165204913 24 21.10278287217925 32.79842117853441 30.74515588868511 15.35364006060123 25 27.556574618682085 25.303558967201674 26.87269464659555 20.267171767520686 26 24.402114291413184 24.027126757754065 27.92061434127855 23.650144609554204 27 21.76323925401416 23.416774708287626 27.82686745786377 26.993118579834448 28 21.009577015163607 32.1927374301676 30.28930566640064 16.508379888268156 29 27.10666586812002 25.541514443712444 31.033518995428324 16.318300692739214 30 20.237381608919794 29.916476841306 37.61939016105183 12.226751388722375 31 21.708277530133728 31.209147060587284 35.60277450176905 11.479800907509945 32 19.993202311214187 27.07479456984625 41.78779215066877 11.144210968270787 33 16.473693682884395 28.85795988561601 37.064810926470294 17.603535505029296 34 15.756 34.114 39.298 10.832 35 14.224836444391093 32.84516735690134 43.42676510013405 9.503231098573515 36 13.637182489641505 34.35617206109009 39.72057086811186 12.28607458115655 37 12.50350322296513 29.729351002922687 46.32461864915722 11.442527124954958 38 16.599911883686467 29.84339327912845 43.68566507790283 9.871029759282253 39 12.258310124426457 30.46344346711014 42.07056843455088 15.20767797391252 40 12.274932830733448 30.1559931026186 47.756345991899586 9.812728074748366 41 11.638726237266383 31.132188978904306 47.07227079489853 10.156813988930777 42 15.43213590869888 30.72186453256313 42.39525041619031 11.450749142547686 43 12.56170486013565 30.58353734398202 46.78532728659148 10.069430509290846 44 11.587862246126676 30.059404350967327 43.82676406839528 14.525969334510716 45 11.624779187409667 31.95358920828649 46.51116107274771 9.910470531556125 46 11.52440249045993 31.986342639084153 41.48222534645511 15.007029524000803 47 11.975807261840176 36.51616533043985 42.48397532501457 9.024052082705406 48 12.985002613485586 36.57874633106831 41.194563949981905 9.241687105464196 49 11.464571359672039 37.68538242032073 41.328724729713436 9.521321490293797 50 15.25468036759235 33.652395986245445 41.64169800317722 9.451225642984978 51 10.726991337205796 37.97961931943802 42.29091713062529 9.00247221273089 52 11.359112022682028 33.946632884920874 44.41696326235145 10.277291830045646 53 12.067890683129889 34.39177911396224 44.11888913467533 9.42144106823255 54 12.333569763907185 38.55709798358497 39.63319485256865 9.476137399939203 55 11.515017401275424 39.49787764791084 39.15186384759299 9.835241103220744 56 15.934916835944572 35.1803061080831 38.65771002192233 10.227067034049998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 6.0 2 12.0 3 6.5 4 11.5 5 22.0 6 187.5 7 353.0 8 339.5 9 326.0 10 309.0 11 292.0 12 289.0 13 429.0 14 572.0 15 783.5 16 995.0 17 1093.5 18 1192.0 19 1418.5 20 1645.0 21 1868.0 22 2510.0 23 2929.0 24 3081.5 25 3234.0 26 3344.5 27 3455.0 28 3733.0 29 3115.5 30 2220.0 31 2353.0 32 2486.0 33 1936.0 34 1386.0 35 1221.5 36 1057.0 37 1006.0 38 988.0 39 1021.0 40 1123.5 41 1226.0 42 2161.0 43 3096.0 44 2255.5 45 1415.0 46 1323.0 47 1448.0 48 1665.0 49 1914.0 50 2163.0 51 1944.0 52 1725.0 53 1574.0 54 1375.0 55 1327.0 56 1249.0 57 1171.0 58 1110.0 59 1049.0 60 848.0 61 647.0 62 558.5 63 389.0 64 308.0 65 266.0 66 224.0 67 197.0 68 170.0 69 149.5 70 129.0 71 104.0 72 63.5 73 48.0 74 34.0 75 20.0 76 20.5 77 21.0 78 16.0 79 7.5 80 4.0 81 3.0 82 2.0 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.025912932546643282 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.007973210014351778 25 0.025912932546643282 26 0.06577898261840216 27 0.06577898261840216 28 0.09567852017222134 29 0.15348429277627174 30 0.24318290543772922 31 0.28105565300590013 32 0.30098867804177964 33 0.3209217030776591 34 0.3348748206027747 35 0.3687609631637698 36 0.41660022324988044 37 0.4265667357678201 38 0.46643278583957903 39 0.5162653484292776 40 0.5860309360548557 41 0.5979907510763833 42 0.6199170786158508 43 0.6657630361983735 44 0.6777228512199012 45 0.7016424812629565 46 0.753468346356243 47 0.7993143039387658 48 0.8491468665284644 49 0.8092808164567055 50 0.8750597990751077 51 0.8272205389889971 52 0.8710731940679317 53 0.8790464040822836 54 1.6404879604528781 55 0.9149258491468665 56 0.8910062191038113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 50168.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.06498166161696 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32307692307693 41.47663849465795 2 13.328506787330316 14.678679636421624 3 5.09683257918552 8.419709775155477 4 2.2805429864253393 5.02312230904162 5 1.1981900452488687 3.298915643438048 6 0.7601809954751132 2.51156115452081 7 0.5248868778280543 2.0232020411417637 8 0.32941176470588235 1.4511242226120236 9 0.19547511312217195 0.9687450167437409 >10 0.8796380090497737 8.457582522723648 >50 0.039819004524886875 1.5547759527985967 >100 0.03257918552036199 2.946101100302982 >500 0.007239819004524887 3.5440918513793656 >1k 0.0036199095022624436 3.6457502790623506 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1829 3.6457502790623506 No Hit TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 980 1.9534364535161859 No Hit TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 798 1.5906553978631797 No Hit TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 269 0.5361983734651571 No Hit TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 267 0.5322117684579811 No Hit TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 175 0.3488279381278903 No Hit TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 169 0.3368681231063626 No Hit TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.2830489555094881 No Hit TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 123 0.2451762079413172 No Hit TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.22922978791261361 No Hit TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.22125657789826186 No Hit TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.21328336788391006 No Hit TATGTAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC 98 0.19534364535161858 No Hit TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 95 0.18936373784085472 No Hit TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.17740392281932704 No Hit TATGTAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.143517780258332 No Hit TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA 70 0.1395311752511561 No Hit TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.13753787274756818 No Hit TATGTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.13155796523680433 No Hit TATGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.1116249402009249 No Hit TATGTAGGGAAAGGGCAAGGCTAAGAAGCATCCTGAATCGGAGCCTGCAGAGGGTT 56 0.1116249402009249 No Hit TATGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 55 0.10963163769733696 No Hit TATGTAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.107638335193749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.011959815021527667 0.0 0.0 0.0 0.0 7 0.013953117525115612 0.0 0.0 0.0 0.0 8 0.015946420028703556 0.0 0.0 0.0 0.0 9 0.0179397225322915 0.0 0.0 0.0 0.0 10 0.037872747568170946 0.0 0.0 0.0 0.0 11 0.045845957582522724 0.0 0.0 0.0 0.0 12 0.0538191675968745 0.0 0.0 0.0 0.0 13 0.06378568011481422 0.0 0.0 0.0 0.0 14 0.07574549513634189 0.0 0.0 0.0 0.0 15 0.07973210014351778 0.0 0.0 0.0 0.0 16 0.0896986126614575 0.0 0.0 0.0 0.0 17 0.09767182267580928 0.0 0.0 0.0 0.0 18 0.1116249402009249 0.0 0.0 0.0 0.0 19 0.12159145271886461 0.0 0.0 0.0 0.0 20 0.1395311752511561 0.0 0.0 0.0 0.0 21 0.14551108276191996 0.0 0.0 0.0 0.0 22 0.16345080529421144 0.0 0.0 0.0 0.0 23 0.2013235528623824 0.0 0.0 0.0 0.0 24 0.2232498804018498 0.0 0.0 0.0 0.0 25 0.23919630043055334 0.0 0.0 0.0 0.0 26 0.24716951044490512 0.0 0.0 0.0 0.0 27 0.26311593047360865 0.0 0.0 0.0 0.0 28 0.30098867804177964 0.0 0.0 0.0 0.0 29 0.3149417955668952 0.0 0.0 0.0 0.0 30 0.35480784563865414 0.0 0.0 0.0 0.0 31 0.36078775314941797 0.0 0.0 0.0 0.0 32 0.37872747568170945 0.0 0.0 0.0 0.0 33 0.3886939881996492 0.0 0.0 0.0 0.0 34 0.3926805932068251 0.0 0.0 0.0 0.0 35 0.4504863658108755 0.0 0.0 0.0 0.0 36 0.4604528783288152 0.0 0.0 0.0 0.0 37 0.46842608834316696 0.0 0.0 0.0 0.0 38 0.4843725083718705 0.0 0.0 0.0 0.0 39 0.49035241588263434 0.0 0.0 0.0 0.0 40 0.50430553340775 0.0 0.0 0.0 0.0 41 0.5202519534364535 0.0 0.0 0.0 0.0 42 0.5381916759687451 0.0 0.0 0.0 0.0 43 0.5521447934938606 0.0 0.0 0.0 0.0 44 0.576064423536916 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGAT 30 9.364958E-7 50.67614 48 TTTGATC 30 9.364958E-7 50.67614 49 CCGTGCG 20 4.017121E-4 50.31855 41 CGTGCGA 20 4.017121E-4 50.31855 42 TGGTCCT 15 0.008175998 50.318546 44 TAAAATT 15 0.008175998 50.318546 46 TTGTATA 15 0.008241443 50.217304 38 CCGGGCA 15 0.008340339 50.066196 33 CTGTGCG 15 0.008406759 49.965965 28 AACATTC 15 0.008440116 49.916 27 CGAACAT 15 0.008473572 49.86613 25 GAACATT 15 0.008473572 49.86613 26 CGCTGTG 15 0.008473572 49.86613 26 CGTAAAG 15 0.008507126 49.816364 21 CTCATCG 15 0.008507126 49.816364 21 ATCGACC 15 0.008507126 49.816364 24 AGCCCGG 15 0.008540778 49.7667 19 AGGGGAT 15 0.008540778 49.7667 6 GGATGGA 15 0.008540778 49.7667 8 AGGGGAC 40 2.7048372E-9 49.7667 6 >>END_MODULE