FastQCFastQC Report
Fri 17 Jun 2016
SRR1526810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14227
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2671.8767132916285936No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2281.6025866310536305No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1471.03324664370563No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.9559288676460251No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.49905110002108666No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.30224221550572855No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.23898221691150628No Hit
GGTACAGGGAAGTGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.23195332817881492No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA280.1968088845153581No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG260.1827511070499754No Hit
GGTACAGGGAAGCATGTAACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.16869332958459268No Hit
GGTACAGGGCTGTCGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.15463555211920996No Hit
GGTACAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.15463555211920996No Hit
GGTACAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG210.1476066633865186No Hit
GGTACAGGGAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1476066633865186No Hit
GGTACAGGGTACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1476066633865186No Hit
GGTACAGGGATAAAAAAAAATAAAAAACATCTCAGGAGACACTGTTGATTTACTTAATTATGTT210.1476066633865186No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG200.14057777465382723No Hit
GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14057777465382723No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14057777465382723No Hit
GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14057777465382723No Hit
GGTACAGGGAAGTGGTAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13354888592113587No Hit
GGTACAGGGGAAACTTACTTGAGATTTCTTATCCAATGCATGTTTGAAAATTGTCCTACATCCA190.13354888592113587No Hit
GGTACAGGGTGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13354888592113587No Hit
GGTACAGGGATCTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13354888592113587No Hit
GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1265199971884445No Hit
GGTACAGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1265199971884445No Hit
GGTACAGGGGCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTATCCCCATAA180.1265199971884445No Hit
GGTACAGGGAGCTGCTTTAAGTTCCTGATGCCTTGAGGAGATCCCAACAGAATAGATGGTCTTT180.1265199971884445No Hit
GGTACAGGGAAGATGGATAACGAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1265199971884445No Hit
GGTACAGGGAAGCATGGTATCACCCAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11949110845575314No Hit
GGTACAGGGAATTTCTTCTTATTATTATTCATCCTGCCTTTGTAATCCATGGCACCAGTGACTT170.11949110845575314No Hit
GGTACAGGGAAGCATGGTATAACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1124622197230618No Hit
GGTACAGGGAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAA160.1124622197230618No Hit
GGTACAGGGAAGTGGACAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1124622197230618No Hit
GGTACAGGGAAGTATCACCAGAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1124622197230618No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCACTTTTTATA150.10543333099037042No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10543333099037042No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG150.10543333099037042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC1050.058.3404277
AGGGTGT257.954854E-658.3404276
AGGGTAC1050.058.3404276
CAGGGGT1000.058.3404275
CAGGGCA452.5465852E-1158.3404275
ACAGGGT2350.058.3404274
GGGCCAT257.954854E-658.3404277
AGGGGTC150.0044868158.3404246
AGGGGGT150.0044868158.3404246
AGGGGGG201.8873431E-458.3404246
AGGGGCA150.0044868158.3404246
AGGGGAC201.8873431E-458.3404246
GGGTCCG201.8873431E-458.3404247
TACAGGC150.0044868158.3404243
TACAGAG201.8873431E-458.3404243
AGGGTTA150.0044868158.3404246
AGGGTGG351.423723E-858.3404246
AGGGCCA303.361838E-758.3404246
AGGGCAT150.0044868158.3404246
AGGGTCC201.8873431E-458.3404246
Produced by FastQC (version 0.11.5)