FastQCFastQC Report
Fri 17 Jun 2016
SRR1526809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16925
Sequences flagged as poor quality0
Sequence length64
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7134.212703101920236No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3251.9202363367799113No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1961.1580502215657311No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.9689807976366323No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.4194977843426883No Hit
GGTACAGGGGCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTATCCCCATAA600.3545051698670606No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG540.3190546528803545No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG490.2895125553914328No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.2776957163958641No Hit
GGTACAGGGACAGCCCCTCTACCAGCTCCCTTCAAACCAAGTCGCTTAAACTAGAAGTGTAGCT450.2658788774002954No Hit
GGTACAGGGATAAAAAAAAATAAAAAACATCTCAGGAGACACTGTTGATTTACTTAATTATGTT400.2363367799113737No Hit
GGTACAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC330.1949778434268833No Hit
GGTACAGGGGAAACTTACTTGAGATTTCTTATCCAATGCATGTTTGAAAATTGTCCTACATCCA320.18906942392909895No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG310.18316100443131464No Hit
GGTACAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT310.18316100443131464No Hit
GGTACAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG300.1772525849335303No Hit
GGTACAGGGAAGTGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1772525849335303No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA270.15952732644017725No Hit
GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1536189069423929No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.14771048744460857No Hit
GGTACAGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.14771048744460857No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1418020679468242No Hit
GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG230.13589364844903987No Hit
GGTACAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG220.12998522895125555No Hit
GGTACAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA220.12998522895125555No Hit
GGTACAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC220.12998522895125555No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCACTTTTTATA220.12998522895125555No Hit
GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12998522895125555No Hit
GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.11816838995568685No Hit
GGTACAGGGAGCTGCTTTAAGTTCCTGATGCCTTGAGGAGATCCCAACAGAATAGATGGTCTTT200.11816838995568685No Hit
GGTACAGGGAAGTGGACAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.11816838995568685No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG190.11225997045790251No Hit
GGTACAGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG190.11225997045790251No Hit
GGTACAGGGAACTGGATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11225997045790251No Hit
GGTACAGGGAAGCATGTAACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11225997045790251No Hit
GGTACAGGGCTGTCGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11225997045790251No Hit
GGTACAGGGGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAG180.10635155096011817No Hit
GGTACAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10635155096011817No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT180.10635155096011817No Hit
GGTACAGGGATCTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10044313146233383No Hit
GGTACAGGGAAGATGGATAACGAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10044313146233383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA258.314841E-657.9911276
AGGGGCA258.314841E-657.9911276
TCAAGAT452.7284841E-1157.99112719
GAAGCAT501.8189894E-1257.9911279
AGGGCTG351.517219E-857.9911276
AGGGTGG258.314841E-657.9911276
AGGGCCT258.314841E-657.9911276
AGGGCAG501.8189894E-1257.9911276
AGGGTAC900.057.9911276
CATCAAG501.8189894E-1257.99112717
AGGGATT258.314841E-657.9911276
AGGGATC258.314841E-657.9911276
CAGGGTG700.057.9911275
CAGGGTC351.517219E-857.9911275
CAGGGCT501.8189894E-1257.9911275
CAGGGCC501.8189894E-1257.9911275
CCATCAA501.8189894E-1257.99112716
GGGACAT258.314841E-657.9911277
GGGACAG258.314841E-657.9911277
GCCATCA501.8189894E-1257.99112715
Produced by FastQC (version 0.11.5)