FastQCFastQC Report
Fri 17 Jun 2016
SRR1526808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16771
Sequences flagged as poor quality0
Sequence length64
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6423.8280364915628167No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3061.8245781408383521No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1761.0494305646651958No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.9003637230934352No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.37564844076083714No Hit
GGTACAGGGGCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTATCCCCATAA550.32794705145787373No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG420.2504322938405581No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.2504322938405581No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG380.2265815991890764No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.21465625186333553No Hit
GGTACAGGGGAAACTTACTTGAGATTTCTTATCCAATGCATGTTTGAAAATTGTCCTACATCCA350.2086935782004651No Hit
GGTACAGGGATAAAAAAAAATAAAAAACATCTCAGGAGACACTGTTGATTTACTTAATTATGTT330.19676823087472423No Hit
GGTACAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG320.1908055572118538No Hit
GGTACAGGGAAGCATGTAACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.17888020988611292No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA290.1729175362232425No Hit
GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1729175362232425No Hit
GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.16099218889750164No Hit
GGTACAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC270.16099218889750164No Hit
GGTACAGGGAAGATGGATAACGAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.1550295152346312No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCACTTTTTATA240.14310416790889036No Hit
GGTACAGGGACAGCCCCTCTACCAGCTCCCTTCAAACCAAGTCGCTTAAACTAGAAGTGTAGCT240.14310416790889036No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.14310416790889036No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.14310416790889036No Hit
GGTACAGGGCTGTCGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.14310416790889036No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG230.1371414942460199No Hit
GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.1371414942460199No Hit
GGTACAGGGAATTTCTTCTTATTATTATTCATCCTGCCTTTGTAATCCATGGCACCAGTGACTT230.1371414942460199No Hit
GGTACAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG220.13117882058314947No Hit
GGTACAGGGAAGTGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.13117882058314947No Hit
GGTACAGGGGTGCTGTAAATTACAGCCTTAGCTTTGATGCAAGAGGTTGCGACACGCTCGGTTG220.13117882058314947No Hit
GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG190.1132907995945382No Hit
GGTACAGGGGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAG190.1132907995945382No Hit
GGTACAGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10732812593166777No Hit
GGTACAGGGACTGATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10136545226879733No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG170.10136545226879733No Hit
GGTACAGGGACTGGACAACGCAGAGTCGACTTTTTCTTTTTTTTTCTTTTTTTTTTTTTTTTTT170.10136545226879733No Hit
GGTACAGGGAAGTGGACAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10136545226879733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCAGG150.00452772958.25301422
AGGATCA150.00452772958.25301426
GAGCCAG150.00452772958.25301421
GCCAGGA150.00452772958.25301423
CAGGATC150.00452772958.25301425
CCAGGAT150.00452772958.25301424
GCGTTGC150.00463717757.9041935
CGTTGCC150.00463717757.9041936
GTTGCCG201.9693296E-457.9041937
AGGGGTC303.6569327E-757.7313466
AGGGGCA406.7666406E-1057.7313466
AGGGGAC150.00469263457.7313466
GGACTGG150.00469263457.7313468
AGGGTGT150.00469263457.7313466
AGGGTGC201.998824E-457.7313466
AGGGCCA150.00469263457.7313466
AGGGCAT303.6569327E-757.7313466
AGGGTAC850.057.7313466
AGGGATA201.998824E-457.7313466
AGGGAGC303.6569327E-757.7313466
Produced by FastQC (version 0.11.5)