FastQCFastQC Report
Fri 17 Jun 2016
SRR1526807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14394
Sequences flagged as poor quality0
Sequence length56
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6994.856190079199667No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3592.4940947617062665No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1701.1810476587467No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1561.0837849103793247No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.38905099346950117No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.38210365430040294No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.2778935667639294No Hit
GGTACAGGGGCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTAT390.27094622759483117No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC340.23620953174933998No Hit
GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC330.22926219258024177No Hit
GGTACAGGGAAGTGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.20842017507294708No Hit
GGTACAGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.20842017507294708No Hit
GGTACAGGGAAGATGGATAACGAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTT290.20147283590384882No Hit
GGTACAGGGATAAAAAAAAATAAAAAACATCTCAGGAGACACTGTTGATTTACTTA290.20147283590384882No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG280.19452549673475059No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG280.19452549673475059No Hit
GGTACAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC250.1736834792274559No Hit
GGTACAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC250.1736834792274559No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1736834792274559No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA240.16673614005835766No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.15978880088925942No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC230.15978880088925942No Hit
GGTACAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG230.15978880088925942No Hit
GGTACAGGGACAGCCCCTCTACCAGCTCCCTTCAAACCAAGTCGCTTAAACTAGAA220.15284146172016116No Hit
GGTACAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG220.15284146172016116No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCAC220.15284146172016116No Hit
GGTACAGGGGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC210.14589412255106293No Hit
GGTACAGGGGTGCTGTAAATTACAGCCTTAGCTTTGATGCAAGAGGTTGCGACACG210.14589412255106293No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA190.13199944421286647Illumina Single End Adapter 1 (95% over 21bp)
GGTACAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA190.13199944421286647No Hit
GGTACAGGGAAGTGGACAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT190.13199944421286647No Hit
GGTACAGGGGAAACTTACTTGAGATTTCTTATCCAATGCATGTTTGAAAATTGTCC190.13199944421286647No Hit
GGTACAGGGTATACGAGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11810476587466999No Hit
GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11115742670557177No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC160.11115742670557177No Hit
GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11115742670557177No Hit
GGTACAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11115742670557177No Hit
GGTACAGGGTGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10421008753647354No Hit
GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10421008753647354No Hit
GGTACAGGGAGATGGCAGAGTCGACTTTTCTTTTTTCTTTTTTTTTTTTTTTTTTT150.10421008753647354No Hit
GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10421008753647354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATTCG150.00783471650.6737648
CAATACA150.00783471650.6737648
ATTCGTA150.00783471650.6737650
AATACAG150.00783471650.6737649
AAGATTA1050.050.67375648
GATTAAG1050.050.67375650
AGATTAA1050.050.67375649
GAGGTAG150.00817120250.1403528
AACAATA150.00817120250.1403546
AAACCGC150.00817120250.1403526
TAGTGAC150.00817120250.1403532
TAAAATT150.00817120250.1403546
TGCGATT150.00817120250.1403536
ATAACAA150.00817120250.1403544
ATTGCGA150.00817120250.1403534
GGTAGTG150.00817120250.1403530
GACGAAA150.00817120250.1403536
AAAATTC150.00817120250.1403547
TATTAGC150.00817120250.1403528
TTGCGAT150.00817120250.1403535
Produced by FastQC (version 0.11.5)