##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526807_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14394 Sequences flagged as poor quality 0 Sequence length 56 %GC 39 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.124982631652074 38.0 38.0 40.0 35.0 40.0 2 37.80693344449076 38.0 38.0 40.0 35.0 40.0 3 36.30186188689732 38.0 35.0 39.0 30.0 40.0 4 37.842642767819925 38.0 38.0 40.0 35.0 40.0 5 37.842156454078086 39.0 38.0 40.0 35.0 40.0 6 37.809503959983324 39.0 38.0 40.0 35.0 40.0 7 38.2850493261081 39.0 38.0 40.0 36.0 40.0 8 38.288245102125885 39.0 38.0 40.0 36.0 40.0 9 38.25149367792135 39.0 38.0 40.0 36.0 40.0 10 37.33937751841045 39.0 37.0 40.0 33.0 40.0 11 37.947339169098235 39.0 38.0 40.0 35.0 40.0 12 37.84354592191191 39.0 38.0 40.0 35.0 40.0 13 37.547728220091706 39.0 38.0 40.0 33.0 40.0 14 37.795956648603585 39.0 38.0 40.0 34.0 40.0 15 37.75989995831596 39.0 38.0 40.0 34.0 40.0 16 37.62303737668473 39.0 38.0 40.0 34.0 40.0 17 37.669862442684455 39.0 38.0 40.0 33.0 40.0 18 37.552938724468525 39.0 38.0 40.0 33.0 40.0 19 37.33673752952619 39.0 37.0 40.0 33.0 40.0 20 37.34757537862998 39.0 37.0 40.0 33.0 40.0 21 37.30693344449076 39.0 37.0 40.0 33.0 40.0 22 37.14005835764902 39.0 37.0 40.0 33.0 40.0 23 37.05891343615395 39.0 36.0 40.0 33.0 40.0 24 36.871474225371685 39.0 36.0 40.0 32.0 40.0 25 36.81499235792691 39.0 36.0 40.0 32.0 40.0 26 36.60490482145338 39.0 36.0 40.0 32.0 40.0 27 36.611296373488955 39.0 36.0 40.0 32.0 40.0 28 36.35563429206614 38.0 36.0 40.0 31.0 40.0 29 36.45706544393497 39.0 36.0 40.0 31.0 40.0 30 36.16729192719188 38.0 35.0 40.0 31.0 40.0 31 36.21703487564263 39.0 36.0 40.0 31.0 40.0 32 35.96276226205363 39.0 35.0 40.0 30.0 40.0 33 36.047867166875086 39.0 35.0 40.0 30.0 40.0 34 36.22995692649715 39.0 36.0 40.0 31.0 40.0 35 35.897179380297345 39.0 35.0 40.0 30.0 40.0 36 35.98020008336807 39.0 35.0 40.0 31.0 40.0 37 35.75593997498958 39.0 35.0 40.0 30.0 40.0 38 35.67903293038766 39.0 35.0 40.0 29.0 40.0 39 35.64179519244129 39.0 35.0 40.0 29.0 40.0 40 35.3890509934695 39.0 35.0 40.0 29.0 40.0 41 35.19292760872586 39.0 35.0 40.0 28.0 40.0 42 35.00437682367653 39.0 35.0 40.0 27.0 40.0 43 34.863762678893984 39.0 35.0 40.0 26.0 40.0 44 34.71814644990968 39.0 35.0 40.0 25.0 40.0 45 34.59573433375017 39.0 35.0 40.0 25.0 40.0 46 34.124287897735165 39.0 35.0 40.0 21.0 40.0 47 33.89919410865638 39.0 34.0 40.0 19.0 40.0 48 33.77303042934556 39.0 34.0 40.0 17.0 40.0 49 33.501667361400585 39.0 34.0 40.0 12.0 40.0 50 33.26066416562457 38.0 33.0 40.0 4.0 40.0 51 32.400514103098516 38.0 33.0 39.0 4.0 40.0 52 32.23051271363068 38.0 32.0 39.0 4.0 40.0 53 31.894192024454632 38.0 32.0 39.0 4.0 40.0 54 31.318674447686536 37.0 31.0 39.0 4.0 40.0 55 30.943031818813395 37.0 31.0 39.0 4.0 40.0 56 18.448659163540363 21.0 15.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0411927708146251 1 2 -0.1227142138907098 1 3 -1.3935626814828552 1 4 -1.8129542959374874 1 5 -2.324787665649005 1 6 -2.5417885335952803 1 7 -1.6843553621915035 1 8 -1.1397131346711191 1 9 -0.9656187066901296 1 10 -1.2079483658264962 1 11 -0.8203743851853034 1 12 -2.0545622444782126 1 13 -1.6218877994088103 1 14 -1.6754132655393121 1 15 -1.2241210703395637 1 16 -0.8522687396637068 1 17 -0.6905248929408785 1 18 -1.9414429524723644 1 19 -0.9923884821994022 1 20 -0.2874843293961078 1 21 -0.094354629963874 1 22 -1.9949153787389022 1 23 -0.9167046655492044 1 24 -1.0390554240344017 1 25 -2.134094500270983 1 26 0.3901992965018053 1 27 0.09198892843850359 1 28 0.3381897397653688 1 29 0.7101781738336257 1 30 0.5974637640604143 1 31 2.2096334104737565 1 32 1.622267974788997 1 33 0.714546436290135 1 34 1.655729552893412 1 35 1.4933611789704315 1 36 0.8278590690355472 1 37 0.5173466706029686 1 38 0.893539723581739 1 39 1.385114885114895 1 40 0.5223821346720428 1 41 0.760229313170484 1 42 1.0423748054000228 1 43 1.523794494487781 1 44 0.6726805524074422 1 45 0.8746395527908035 1 46 3.135995913516922 1 47 2.79732829029048 1 48 1.1999949630201812 1 49 1.7514275756922828 1 50 2.9578384150863 1 51 0.7371542090029521 1 52 0.2059475538467268 1 53 1.378250157871996 1 54 2.166153139577503 1 55 2.2429255851524807 1 56 5.0900249027349815 2 1 0.526989047367195 2 2 0.5875130588365636 2 3 1.6064373185171448 2 4 0.8461366131534191 2 5 0.5672577888964483 2 6 0.9638932845865398 2 7 0.8667810014448634 2 8 0.865968683510701 2 9 0.7332449296735035 2 10 0.5193243614462304 2 11 0.21371652390560314 2 12 0.0022559373399673177 2 13 0.15652129150028316 2 14 0.767768552642508 2 15 0.4520152932968031 2 16 0.20454944215447313 2 17 0.06515692524094163 2 18 1.1096934111640024 2 19 0.05306606325514451 2 20 1.3375156706038922 2 21 0.9795090063997591 2 22 0.7948573485338244 2 23 0.9696589708144288 2 24 1.1484445759655983 2 25 0.5477236815471969 2 26 -1.007527976225468 2 27 -0.004601980652402915 2 28 0.3325079215835487 2 29 0.31813271928817244 2 30 1.4099637640604143 2 31 1.1300879559283032 2 32 2.5824952475162704 2 33 0.9588646181083149 2 34 2.150047734711592 2 35 2.345633906243158 2 36 1.4755863417628206 2 37 2.3412103069666017 2 38 2.6605851781271923 2 39 2.0442057942058014 2 40 2.6132912255811362 2 41 3.322729313170484 2 42 3.0139657144909293 2 43 3.6885672217605077 2 44 3.837453279680169 2 45 3.53941228006353 2 46 2.7325868226078285 2 47 2.751873744835933 2 48 2.381813144838361 2 49 3.501427575692283 2 50 1.9578384150863002 2 51 2.3962451180938587 2 52 2.96731119021036 2 53 2.758931976053816 2 54 -0.8508923149679504 2 55 -0.41616532393842576 2 56 1.3286612663713449 3 1 -0.3896776192994693 3 2 -0.9333202744967721 3 3 -1.5602293481495195 3 4 -0.9038633868465809 3 5 -1.2035755444368874 3 6 -0.5569400487467959 3 7 -0.15405233188847234 3 8 -0.071531316489299 3 9 -0.4959217369931608 3 10 -1.8140089718871053 3 11 -0.16128347609439686 3 12 -0.12274406266003268 3 13 0.46902129150028316 3 14 0.4761018859758437 3 15 -0.11048470670319688 3 16 -1.0871172245121912 3 17 -1.705676408092394 3 18 -0.7444732555026619 3 19 -1.9469339367448555 3 20 -0.1208176627294435 3 21 -2.624657660266905 3 22 -2.18430931813284 3 23 -1.363674362518907 3 24 -3.0390554240344017 3 25 -0.7856096517861388 3 26 -3.7991946428921324 3 27 -3.6504353139857386 3 28 -4.313325411749787 3 29 -2.4943672807118276 3 30 -2.2358695692729214 3 31 -2.2449120440716968 3 32 -2.1883380858170653 3 33 -2.436968715225021 3 34 -2.5791189319550725 3 35 -2.279366093756842 3 36 -1.1494136582371794 3 37 -0.929623026366734 3 38 -1.9019148218728077 3 39 -0.5391275391275343 3 40 0.029957892247800544 3 41 -0.739770686829516 3 42 -0.465200952175735 3 43 -2.6655994449061566 3 44 0.0666199463468331 3 45 -0.14808771993646985 3 46 0.8784201559411642 3 47 -0.41479292183073113 3 48 0.9651464781716967 3 49 1.3347609090256185 3 50 2.2078384150863 3 51 -0.4370882152394735 3 52 0.09231119021035994 3 53 0.11309864272048387 3 54 0.9616076850320496 3 55 0.33383467606157424 3 56 1.536994599704677 4 1 -1.0801538097756591 4 2 -1.5642726554491517 4 3 -1.9649912529114246 4 4 -1.4752919582751503 4 5 -0.7988136396749823 4 6 -1.5807495725563143 4 7 -1.035004712840852 4 8 -1.2501027450607296 4 9 -1.6149693560407812 4 10 -2.552104209982339 4 11 -1.0541406189515428 4 12 -1.5156012055171786 4 13 -2.030978708499717 4 14 -1.7143743045003461 4 15 -0.717627563846051 4 16 -1.027593414988381 4 17 -2.158057360473343 4 18 -0.7920923031217129 4 19 -2.518362508173425 4 20 -0.2874843293961078 4 21 -1.5294195650288103 4 22 -3.4104997943233215 4 23 -1.2803410291855712 4 24 -3.9676268526058323 4 25 -1.8094191755956572 4 26 -2.882527976225468 4 27 -2.459959123509549 4 28 -1.765706364130736 4 29 -1.9586529949975429 4 30 -2.473964807368155 4 31 0.040802241642587944 4 32 0.39499524751627035 4 33 -0.10363538189168509 4 34 0.4089763061401612 4 35 0.29206247767172755 4 36 0.24344348461996645 4 37 0.7965674498237476 4 38 1.4195137495557617 4 39 1.579920079920086 4 40 0.5418626541525668 4 41 1.760229313170484 4 42 1.237180000205214 4 43 1.6796386503319383 4 44 2.0428104225373147 4 45 1.6733408514920995 4 46 2.330801108322113 4 47 2.037588030550218 4 48 1.7389560019812151 4 49 3.251427575692283 4 50 -1.6850187277708422 4 51 -0.13946916762042605 4 52 -0.15768880978964006 4 53 0.053574833196673666 4 54 0.8544648278891955 4 55 0.6195489617758589 4 56 -0.1891958764857975 5 1 0.5462198165979615 5 2 -0.15767924885574303 5 3 0.9910527031325316 5 4 0.24998276699957245 5 5 0.975911635050295 5 6 -0.03129902310576682 5 7 0.6792810014448634 5 8 0.6015456065876208 5 9 0.6707449296735035 5 10 0.2885551306769969 5 11 -0.06512962994055016 5 12 -1.2573594472754195 5 13 0.08440590688489635 5 14 0.4504608603348146 5 15 0.4472076009891097 5 16 0.17570328830831983 5 17 -0.25695845937444517 5 18 -0.03934505037445746 5 19 -1.1392416290525489 5 20 0.3279002859885054 5 21 0.23431669870745253 5 22 -0.036873420696942105 5 23 -0.7418794907240311 5 24 0.11479072981175165 5 25 1.124646758470277 5 26 -0.03637413007162138 5 27 0.029051865501443785 5 28 0.9815463831220086 5 29 1.2556327192881724 5 30 1.0590022255988742 5 31 1.4473956482359966 5 32 0.6642260167470369 5 33 0.5117492334929281 5 34 0.8375477347115918 5 35 -0.7793660937568418 5 36 0.40827864945512715 5 37 0.2306333838896819 5 38 -0.036530206488194494 5 39 0.6788211788211882 5 40 1.0748296871195961 5 41 -0.5474629945218226 5 42 0.4130041760293892 5 43 0.17414414483742746 5 44 0.1691840489109353 5 45 -0.7827031045518567 5 46 0.04508682260782848 5 47 0.48264297560516667 5 48 -0.07972531670009886 5 49 1.4437352679999762 5 50 1.9578384150863002 5 51 -0.04606257421383475 5 52 2.3807727286719 5 53 1.4272012068230495 5 54 2.78853076195513 5 55 2.5646039068308077 5 56 2.1459689586790383 6 1 -0.15634428596613503 6 2 -0.9166536078301064 6 3 -0.660229348149521 6 4 0.296136613153422 6 5 -0.40357554443688315 6 6 0.07639328458653694 6 7 0.2792810014448648 6 8 0.14513535017736956 6 9 0.27074492967350494 6 10 -1.147342305220434 6 11 -0.5779501427610612 6 12 -0.27274406266003126 6 13 -1.0643120418330483 6 14 -1.1238981140241577 6 15 0.13951529329680312 6 16 0.26288277548780314 6 17 0.5276569252409402 6 18 0.2221934111639996 6 19 0.35306606325514167 6 20 -0.020817662729442077 6 21 -0.5246576602669109 6 22 0.3323573485338258 6 23 -0.547007695852237 6 24 -0.9723887573677317 6 25 -2.4856096517861346 6 26 -0.8825279762254681 6 27 -0.5504353139857372 6 28 0.12000792158355011 6 29 0.12229938595483958 6 30 -1.6025362359395885 6 31 1.2884212892616347 6 32 1.8949952475162704 6 33 1.0296979514416478 6 34 1.1042144013782575 6 35 0.420633906243161 6 36 2.1005863417628206 6 37 1.887043640299936 6 38 2.1480851781271895 6 39 1.3608724608724714 6 40 1.5466245589144663 6 41 2.0268959798371498 6 42 1.6181323811575936 6 43 2.851067221760509 6 44 2.266619946346836 6 45 2.801912280063533 6 46 2.711753489274493 6 47 1.9852070781692674 6 48 3.8484798115050296 6 49 3.418094242358947 6 50 3.224505081752966 6 51 1.24624511809386 6 52 2.5089778568770242 6 53 1.3297653093871489 6 54 2.0449410183653853 6 55 1.467168009394907 6 56 1.8536612663713434 7 1 -0.22301095263280502 7 2 -0.18332027449677213 7 3 0.40643731851714193 7 4 0.6294699464867506 7 5 0.6630911222297797 7 6 -0.056940048746795924 7 7 0.07928100144486194 7 8 0.14513535017736956 7 9 0.27074492967350494 7 10 0.05265769477956184 7 11 0.4887165239056017 7 12 -0.1394107293266984 7 13 0.06902129150028458 7 14 0.076101885975838 7 15 0.2061819599634731 7 16 -0.40378389117886115 7 17 -1.139009741425724 7 18 0.35552674449733246 7 19 -1.1802672700781898 7 20 -0.48748432939611064 7 21 -0.6579909936002437 7 22 0.26569068186715583 7 23 0.5863256374810959 7 24 -0.9057220907010688 7 25 -1.6856096517861374 7 26 -0.01586130955880094 7 27 -1.0171019806524058 7 28 0.9200079215835473 7 29 -0.5443672807118247 7 30 0.5307970973937444 7 31 0.9550879559283061 7 32 1.9616619141829403 7 33 0.6296979514416492 7 34 0.37088106804492327 7 35 1.2873005729098281 7 36 0.16725300842949054 7 37 -0.11295635970006401 7 38 -0.9185814885394734 7 39 0.027539127539135677 7 40 -0.45337544108553374 7 41 -0.9064373534961803 7 42 0.08479904782426217 7 43 0.31773388842717765 7 44 -0.6667133869865012 7 45 0.20191228006353157 7 46 1.511753489274497 7 47 1.385207078169266 7 48 1.0484798115050253 7 49 0.9514275756922856 7 50 2.89117174841963 7 51 1.8462451180938615 7 52 1.2423111902103585 7 53 2.5964319760538146 7 54 2.0449410183653853 7 55 3.66716800939491 7 56 1.5203279330380113 8 1 0.11032238070053069 8 2 -1.2666536078301007 8 3 1.3564373185171448 8 4 -0.6538633868465809 8 5 0.6297577888964483 8 6 0.8597266179198755 8 7 1.012614334778199 8 8 0.5951353501773653 8 9 0.08741159634016782 8 10 1.6859910281128947 8 11 1.1720498572389388 8 12 0.6272559373399673 8 13 0.8023546248336189 8 14 -2.6905647806908277 8 15 1.2228486266301388 8 16 -0.5871172245121912 8 17 -0.7056764080923941 8 18 -0.49447325550266186 8 19 0.8030660632551445 8 20 -1.7874843293961078 8 21 0.12534233973309483 8 22 0.5656906818671601 8 23 0.8029923041477645 8 24 -0.7890554240344017 8 25 0.46439034821386116 8 26 1.5341386904411962 8 27 -0.8171019806524029 8 28 1.186674588250213 8 29 1.5889660526215081 8 30 -0.48586956927292135 8 31 0.005087955928303245 8 32 0.978328580849606 8 33 -0.7703020485583494 8 34 -0.9957855986217439 8 35 0.22063390624315815 8 36 -0.14941365823717945 8 37 0.2370436402999374 8 38 0.681418511460528 8 39 0.3775391275391371 8 40 0.5299578922478005 8 41 1.260229313170484 8 42 1.3681323811575936 8 43 1.667733888427172 8 44 1.4832866130135045 8 45 1.8519122800635301 8 46 1.7117534892744928 8 47 1.8352070781692689 8 48 -0.7015201884949747 8 49 0.2514275756922828 8 50 0.2078384150863002 8 51 0.6462451180938587 8 52 0.7589778568770242 8 53 1.0297653093871517 8 54 0.8782743516987139 8 55 2.0838346760615742 8 56 1.536994599704677 9 1 0.3769890473671964 9 2 0.750013058836565 9 3 0.2064373185171462 9 4 0.7628032798200834 9 5 0.8630911222297826 9 6 1.2763932845865398 9 7 0.5459476681115305 9 8 0.745135350177371 9 9 0.7374115963401735 9 10 1.4526576947795604 9 11 0.9553831905722703 9 12 1.393922604006633 9 13 1.4690212915002832 9 14 0.6094352193091765 9 15 1.1395152932968031 9 16 0.26288277548780314 9 17 0.6609902585742731 9 18 0.888860077830671 9 19 1.3530660632551417 9 20 0.9791823372705579 9 21 0.8086756730664248 9 22 -0.06764265146617277 9 23 1.452992304147763 9 24 0.8942779092989284 9 25 1.0477236815471969 9 26 1.5174720237745305 9 27 1.6495646860142656 9 28 1.3866745882502158 9 29 1.722299385954841 9 30 2.064130430727083 9 31 2.221754622594972 9 32 2.228328580849606 9 33 2.896364618108315 9 34 2.637547734711589 9 35 2.153967239576488 9 36 2.567253008429489 9 37 3.2203769736332717 9 38 2.748085178127191 9 39 3.2275391275391314 9 40 3.4132912255811334 9 41 0.026895979837149753 9 42 0.8181323811575965 9 43 0.5844005550938434 9 44 1.266619946346836 9 45 1.6019122800635301 9 46 2.711753489274493 9 47 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119 23 0.5946589708144288 119 24 0.21094457596559835 119 25 -0.5147763184528031 119 26 0.05497202377453192 119 27 0.8078980193475971 119 28 1.1450079215835487 119 29 0.8181327192881724 119 30 1.0974637640604143 119 31 1.3800879559283032 119 32 2.0199952475162704 119 33 2.083864618108315 119 34 1.8375477347115918 119 35 1.9706339062431582 119 36 1.4130863417628206 119 37 0.7787103069666017 119 38 1.5355851781271923 119 39 2.4192057942058014 119 40 0.42579122558113625 119 41 0.510229313170484 119 42 0.9514657144909293 119 43 1.3760672217605077 119 44 1.5249532796801688 119 45 -0.9605877199364699 119 46 0.6075868226078285 119 47 -1.1231262551640668 119 48 0.38181314483836104 119 49 -0.3110724243077172 119 50 -0.7296615849136998 119 51 0.02124511809385865 119 52 0.9048111902103599 119 53 0.946431976053816 119 54 2.2116076850320496 119 55 1.7088346760615742 119 56 0.6411612663713449 120 1 0.776989047367195 120 2 -0.13570122687772113 120 3 1.7492944613742907 120 4 1.0961366131534191 120 5 1.2011863603250177 120 6 0.7049647131579704 120 7 0.6792810014448634 120 8 0.678468683510701 120 9 0.6707449296735035 120 10 0.6621815043033763 120 11 0.8744308096198878 120 12 0.5558273659113979 120 13 1.1833070057859985 120 14 0.7856256954996539 120 15 -0.5033418495603428 120 16 0.8295494421544731 120 17 0.7705140680980875 120 18 1.0650505540211483 120 19 0.9816374918265751 120 20 1.1410870991753228 120 21 1.0420090063997591 120 22 -0.05335693718046031 120 23 1.0053732565287135 120 24 1.5323731473941677 120 25 1.6905808244043428 120 26 1.1889005952031013 120 27 -0.3171019806524029 120 28 -0.5514206498450207 120 29 1.3984898621453183 120 30 1.5974637640604143 120 31 2.183659384499734 120 32 1.2521381046591245 120 33 2.1820789038225996 120 34 2.194690591854446 120 35 1.5063481919574429 120 36 2.2434434846199665 120 37 2.0822817355380323 120 38 2.490942320984338 120 39 2.0084915084915167 120 40 3.041862654152567 120 41 3.0459435988847687 120 42 3.4514657144909293 120 43 3.1082100789033618 120 44 3.471381851108738 120 45 3.3161979943492454 120 46 1.7593725368935438 120 47 3.251873744835933 120 48 2.8103845734097916 120 49 3.5371418614065675 120 50 3.5292669865148696 120 51 1.5033879752367127 120 52 0.12802547592464464 120 53 2.6250034046252466 120 54 3.7116076850320496 120 55 3.6909775332044283 120 56 3.025089837799918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 0.0 6 3.0 7 6.0 8 12.0 9 7.0 10 12.0 11 7.0 12 13.0 13 20.0 14 21.0 15 22.0 16 33.0 17 34.0 18 56.0 19 53.0 20 61.0 21 60.0 22 69.0 23 90.0 24 113.0 25 123.0 26 128.0 27 139.0 28 190.0 29 216.0 30 271.0 31 360.0 32 458.0 33 565.0 34 758.0 35 949.0 36 1242.0 37 2243.0 38 3085.0 39 2967.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.02737251632625 0.854522717799083 0.09726274836737529 0.020842017507294707 8 99.14518034609772 0.41003544374174716 0.2293418583640281 0.21544235179651122 9 97.79769348339586 1.3477837988050576 0.36126163679310824 0.4932610810059747 10 27.63651521467278 41.10045852438516 15.409198277059884 15.853827983882173 11 23.27358621647909 41.19772127275254 19.987494789495624 15.541197721272754 12 43.40697512852578 18.007503126302627 17.889398360427954 20.696123384743643 13 23.33611226900097 16.437404474086424 28.359038488258996 31.86744476865361 14 26.351257468389605 26.22620536334584 26.73336112269001 20.689176045574545 15 38.481311657635125 22.738641100458523 21.161595109073225 17.618452132833127 16 27.073780741975824 24.55884396276226 24.13505627344727 24.232319021814643 17 25.684312908156176 25.35084062803946 23.77379463665416 25.1910518271502 18 24.99652633041545 27.983882173127693 25.87189106572183 21.14770043073503 19 26.115047936640266 27.719883284701957 23.933583437543422 22.231485341114354 20 21.856329025983047 34.674169792969295 18.973183270807283 24.49631791024038 21 21.800750312630264 22.960955953869668 31.44365707933861 23.794636654161454 22 22.703904404613034 25.39252466305405 22.717799082951228 29.185771849381688 23 25.767680978185354 27.671251910518276 21.70348756426289 24.857579547033488 24 20.439071835487006 30.846185910796166 29.817979713769628 18.8967625399472 25 28.592273485269594 22.1650917176209 26.785714285714285 22.45692051139522 26 23.043780403057678 20.34051424600417 29.541348158443366 27.074357192494787 27 16.875173755907703 20.33639143730887 31.55407283847651 31.234361968306924 28 17.245933546503544 29.27151397191714 34.28333101626582 19.1992214653135 29 25.577351140790206 20.12381747356706 36.86700055648303 17.43183082915971 30 13.994432846207378 24.585942936673625 46.36047320807237 15.059151009046627 31 17.22601309009887 25.463027433505083 43.86575685837627 13.445202618019774 32 17.252663092668662 17.983708138968183 51.228851911160625 13.534776857202536 33 11.127358819023744 19.8105981477613 46.76554557482069 22.296497458394263 34 12.435558032604153 25.35878500766337 49.95819980493243 12.247457154800054 35 11.378997979234896 22.79980489164518 56.755626785589854 9.065570343530068 36 12.294396431558404 24.81182046278227 50.10454418734318 12.789238918316142 37 10.867898222377134 17.92262112234228 59.59567793656326 11.613802718717322 38 16.366806136680616 19.205020920502093 54.3305439330544 10.097629009762901 39 10.691867763983819 19.89817268796206 53.57790486818246 15.832054679871671 40 10.961390770090066 18.857781191091252 59.78496125113454 10.395866787684144 41 11.935191004958448 19.631259166142886 58.22333961868845 10.21021021021021 42 15.682850157177786 17.394341599720573 54.11107230178135 12.811735941320293 43 10.573025856044724 18.88888888888889 59.727463312368975 10.810621942697415 44 10.404583886520859 19.06225980015373 54.4057019076235 16.127454405701908 45 11.759770677480248 19.653219604278824 58.617073341257075 9.96993637698385 46 10.796447800853088 19.70491574015803 53.199076987623236 16.299559471365637 47 10.513430330162283 26.140179071068832 53.89619473978735 9.450195858981534 48 11.589334453075791 26.78983833718245 51.97004688921548 9.65078032052628 49 10.20008395130824 27.710927661956063 51.91688820484119 10.172100181894502 50 15.937259295567538 21.74217491772285 52.81843008192703 9.502135704782578 51 11.033323998879865 26.47717726127135 53.2343881265752 9.255110613273592 52 12.039501330718588 21.81678106177336 55.287855441938646 10.855862165569407 53 11.62546396806499 22.60662511380349 53.925344912108685 11.842566006022832 54 12.412624443973733 28.00960248534915 49.961166419543886 9.616606651133235 55 11.20925341745531 29.078163336838415 48.98703119523309 10.725552050473187 56 17.06377014716188 23.34968465311843 48.36720392431675 11.219341275402943 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 14.0 11 26.0 12 26.5 13 30.5 14 34.0 15 52.5 16 71.0 17 137.5 18 204.0 19 290.0 20 376.0 21 339.5 22 353.0 23 403.0 24 564.5 25 726.0 26 824.5 27 923.0 28 1017.0 29 1226.0 30 1341.0 31 1184.0 32 1027.0 33 770.0 34 513.0 35 609.0 36 705.0 37 504.0 38 225.0 39 147.0 40 158.5 41 170.0 42 198.0 43 226.0 44 258.0 45 290.0 46 683.0 47 705.0 48 334.0 49 366.5 50 399.0 51 404.5 52 410.0 53 448.5 54 449.5 55 412.0 56 400.5 57 389.0 58 413.5 59 438.0 60 385.5 61 333.0 62 310.0 63 250.0 64 213.0 65 208.0 66 203.0 67 171.0 68 139.0 69 118.5 70 98.0 71 96.0 72 75.5 73 57.0 74 49.0 75 41.0 76 29.0 77 17.0 78 17.5 79 14.0 80 10.0 81 8.0 82 6.0 83 5.5 84 5.0 85 2.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.034736695845491175 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.013894678338196471 26 0.027789356676392943 27 0.041684035014589414 28 0.055578713352785886 29 0.12505210504376824 30 0.16673614005835766 31 0.22231485341114354 32 0.21536751424204528 33 0.22926219258024177 34 0.2778935667639294 35 0.29873558427122415 36 0.31957760177851885 37 0.34041961928581355 38 0.3751563151313047 39 0.38905099346950117 40 0.4932610810059747 41 0.5210504376823677 42 0.5488397943587605 43 0.5835764902042517 44 0.5766291510351536 45 0.6322078643879394 46 0.6461025427261359 47 0.6808392385716271 48 0.7294706127553148 49 0.6947339169098236 50 0.7850493261081006 51 0.7642073086008059 52 0.8058913436153953 53 0.798944004446297 54 1.6048353480616924 55 0.8962067528136725 56 0.8614700569681811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 14394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.55537029317772 #Duplication Level Percentage of deduplicated Percentage of total 1 71.07808776111033 35.22300958732805 2 14.201598205523622 14.075309156593024 3 5.341371092107107 7.940808670279282 4 3.0702369269592036 6.085869112130053 5 1.7243796439085939 4.272613588995415 6 1.3037992429552783 3.8766152563568155 7 0.8131221085097435 2.8206197026538837 8 0.39254170755642787 1.5562039738780047 9 0.32244497406420863 1.4380992080033346 >10 1.6683022571148183 12.32457968598027 >50 0.028038693396887703 0.7711546477699042 >100 0.04205804009533156 4.758927330832291 >500 0.014019346698443852 4.856190079199667 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 699 4.856190079199667 No Hit GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 359 2.4940947617062665 No Hit GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 170 1.1810476587467 No Hit GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 156 1.0837849103793247 No Hit GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.38905099346950117 No Hit GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.38210365430040294 No Hit GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.2778935667639294 No Hit GGTACAGGGGCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTAT 39 0.27094622759483117 No Hit GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 34 0.23620953174933998 No Hit GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 33 0.22926219258024177 No Hit GGTACAGGGAAGTGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.20842017507294708 No Hit GGTACAGGGTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.20842017507294708 No Hit GGTACAGGGAAGATGGATAACGAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTT 29 0.20147283590384882 No Hit GGTACAGGGATAAAAAAAAATAAAAAACATCTCAGGAGACACTGTTGATTTACTTA 29 0.20147283590384882 No Hit GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 28 0.19452549673475059 No Hit GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 28 0.19452549673475059 No Hit GGTACAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC 25 0.1736834792274559 No Hit GGTACAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 25 0.1736834792274559 No Hit GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.1736834792274559 No Hit GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 24 0.16673614005835766 No Hit GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.15978880088925942 No Hit GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 23 0.15978880088925942 No Hit GGTACAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 23 0.15978880088925942 No Hit GGTACAGGGACAGCCCCTCTACCAGCTCCCTTCAAACCAAGTCGCTTAAACTAGAA 22 0.15284146172016116 No Hit GGTACAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG 22 0.15284146172016116 No Hit GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCAC 22 0.15284146172016116 No Hit GGTACAGGGGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC 21 0.14589412255106293 No Hit GGTACAGGGGTGCTGTAAATTACAGCCTTAGCTTTGATGCAAGAGGTTGCGACACG 21 0.14589412255106293 No Hit GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 19 0.13199944421286647 Illumina Single End Adapter 1 (95% over 21bp) GGTACAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA 19 0.13199944421286647 No Hit GGTACAGGGAAGTGGACAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.13199944421286647 No Hit GGTACAGGGGAAACTTACTTGAGATTTCTTATCCAATGCATGTTTGAAAATTGTCC 19 0.13199944421286647 No Hit GGTACAGGGTATACGAGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.11810476587466999 No Hit GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.11115742670557177 No Hit GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 16 0.11115742670557177 No Hit GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.11115742670557177 No Hit GGTACAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.11115742670557177 No Hit GGTACAGGGTGGCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.10421008753647354 No Hit GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.10421008753647354 No Hit GGTACAGGGAGATGGCAGAGTCGACTTTTCTTTTTTCTTTTTTTTTTTTTTTTTTT 15 0.10421008753647354 No Hit GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.10421008753647354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.006947339169098236 0.0 0.0 0.0 0.0 7 0.006947339169098236 0.0 0.0 0.0 0.0 8 0.006947339169098236 0.0 0.0 0.0 0.0 9 0.006947339169098236 0.0 0.0 0.0 0.0 10 0.006947339169098236 0.0 0.0 0.0 0.0 11 0.020842017507294707 0.0 0.0 0.0 0.0 12 0.020842017507294707 0.0 0.0 0.0 0.0 13 0.027789356676392943 0.0 0.0 0.0 0.0 14 0.027789356676392943 0.0 0.0 0.0 0.0 15 0.027789356676392943 0.0 0.0 0.0 0.0 16 0.027789356676392943 0.0 0.0 0.0 0.0 17 0.034736695845491175 0.0 0.0 0.0 0.0 18 0.034736695845491175 0.0 0.0 0.0 0.0 19 0.048631374183687646 0.0 0.0 0.0 0.0 20 0.055578713352785886 0.0 0.0 0.0 0.0 21 0.06252605252188412 0.0 0.0 0.0 0.0 22 0.07642073086008058 0.0 0.0 0.0 0.0 23 0.08336807002917883 0.0 0.0 0.0 0.0 24 0.10421008753647353 0.0 0.0 0.0 0.0 25 0.10421008753647353 0.0 0.0 0.0 0.0 26 0.11810476587467 0.0 0.0 0.0 0.0 27 0.12505210504376824 0.0 0.0 0.0 0.0 28 0.15284146172016116 0.0 0.0 0.0 0.0 29 0.16673614005835766 0.0 0.0 0.0 0.0 30 0.1736834792274559 0.0 0.0 0.0 0.0 31 0.18757815756565235 0.0 0.0 0.0 0.0 32 0.18757815756565235 0.0 0.0 0.0 0.0 33 0.18757815756565235 0.0 0.0 0.0 0.0 34 0.20842017507294705 0.0 0.0 0.0 0.0 35 0.22926219258024177 0.0 0.0 0.0 0.0 36 0.22926219258024177 0.0 0.0 0.0 0.0 37 0.23620953174934 0.0 0.0 0.0 0.0 38 0.23620953174934 0.0 0.0 0.0 0.0 39 0.23620953174934 0.0 0.0 0.0 0.0 40 0.25010421008753647 0.0 0.0 0.0 0.0 41 0.27094622759483117 0.0 0.0 0.0 0.0 42 0.28484090593302763 0.0 0.0 0.0 0.0 43 0.29178824510212586 0.0 0.0 0.0 0.0 44 0.30568292344032233 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATTCG 15 0.007834716 50.67376 48 CAATACA 15 0.007834716 50.67376 48 ATTCGTA 15 0.007834716 50.67376 50 AATACAG 15 0.007834716 50.67376 49 AAGATTA 105 0.0 50.673756 48 GATTAAG 105 0.0 50.673756 50 AGATTAA 105 0.0 50.673756 49 GAGGTAG 15 0.008171202 50.14035 28 AACAATA 15 0.008171202 50.14035 46 AAACCGC 15 0.008171202 50.14035 26 TAGTGAC 15 0.008171202 50.14035 32 TAAAATT 15 0.008171202 50.14035 46 TGCGATT 15 0.008171202 50.14035 36 ATAACAA 15 0.008171202 50.14035 44 ATTGCGA 15 0.008171202 50.14035 34 GGTAGTG 15 0.008171202 50.14035 30 GACGAAA 15 0.008171202 50.14035 36 AAAATTC 15 0.008171202 50.14035 47 TATTAGC 15 0.008171202 50.14035 28 TTGCGAT 15 0.008171202 50.14035 35 >>END_MODULE