FastQCFastQC Report
Fri 17 Jun 2016
SRR1526806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16952
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA1430.843558282208589No Hit
CATCCCAGGAGACTACCTAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT750.4424256724870222No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT540.31854648419065595No Hit
CATCCCGGGATCTCAGTTGATTTTCCAAGTAACTAGATGTAGTTGGCCTCCTCTTCTACCACTT500.2949504483246814No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT370.21826333176026427No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.21236432279377065No Hit
CATCCCGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.206465313827277No Hit
CATCCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG270.15927324209532798No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA270.15927324209532798No Hit
CATCCCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGA260.15337423312883436No Hit
CATCCCGGGAAGTAAGGAGTATGAAGAGCTTAATACGTTACTAAAAAATAAATAGGATATTTCA230.13567720622935348No Hit
CATCCCGGGAGTAATATAAATACCTACTTGCAATTAAACACCTCACTCACTAAAGTCTAATTAT210.12387918829636622No Hit
CATCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG200.11798017932987258No Hit
CATCCCGGGGGGATCTGAACCATGCTGTGGCTAAGGACCCCGTAAAATAACATCTGTCGTTAAG190.11208117036337895No Hit
CATCCCGGGGACTGAGGCTGACTAAGTTGTCCCGAGAACTGACTTTCTCCCCGACCCCGTCCTA180.10618216139688533No Hit
CATCCCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATGCAAAACAG170.1002831524303917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTT258.203699E-658.12166235
GGTCTTT150.00456957858.12166239
TGTTTCG150.00456957858.12166255
AAGATTA150.00456957858.12166248
GTCTTTC150.00456957858.12166240
GCTTGTC258.203699E-658.12166238
TGAGGGT150.00456957858.12166249
CTTGTCT258.203699E-658.12166239
AGATTAA201.9336764E-458.12166249
CTCAAAG150.00456957858.12166244
GTAGCAG150.00456957858.12166236
CGGGGTG303.56471E-757.9497076
CGGGGTA150.004623737757.9497076
TGTCCGG150.004623737757.94970726
CGGGGAA303.56471E-757.9497076
CGGGAGT452.7284841E-1157.9497076
CGGGAAC150.004623737757.9497076
TCCGCTC150.004623737757.94970730
TAGCATA258.350133E-657.94970330
GCCAGTA201.962377E-457.94970325
Produced by FastQC (version 0.11.5)