FastQCFastQC Report
Fri 17 Jun 2016
SRR1526805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23725
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA3681.5511064278187567No Hit
CATCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG3231.3614330874604847No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1760.7418335089567967No Hit
CATCCCGGGGAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTCTTGCTCCTCACGCGG1210.5100105374077977No Hit
CATCCCAGGAGACTACCTAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT930.39199157007376184No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT840.3540569020021075No Hit
CATCCCGGGATCTCAGTTGATTTTCCAAGTAACTAGATGTAGTTGGCCTCCTCTTCTACCACTT790.3329820864067439No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT760.32033719704952585No Hit
CATCCCGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAG690.29083245521601686No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC620.2613277133825079No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAGTTCCTGGG590.2486828240252898No Hit
CATCCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG580.24446786090621705No Hit
CATCCCGGGGGGGGCGTAGCAAAGGGCATAAGGCTGTCTGATCATACGGAAAGTCTTAGCGGAC580.24446786090621705No Hit
CATCCCGGGGGGCGGGAGAGGAACACTTCTCTCAGGCTTGAGCAGAACCTCGCCCAGCCTGTCG560.23603793466807163No Hit
CATCCCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATGCAAAACAG470.19810326659641728No Hit
CATCCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT450.18967334035827188No Hit
CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTTGGTGTGGC450.18967334035827188No Hit
CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA440.18545837723919917No Hit
CATCCCGGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTA410.17281348788198103No Hit
CATCCCGGGGGGCACTGTGGGCAGCCCCAGAGGGCCCTGATCCAAGGCACTTATGTAATCAGGA400.16859852476290835No Hit
CATCCCGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.16438356164383564No Hit
CATCCCGGGAGAAGACCGGGTTCGCGTGTGTGCTGTGTCCGTAGTGGCCTTTTCTGGGCCTCGT390.16438356164383564No Hit
CATCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC370.1559536354056902No Hit
CATCCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG370.1559536354056902No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG360.15173867228661747No Hit
CATCCCGGGGGGATCTGAACCATGCTGTGGCTAAGGACCCCGTAAAATAACATCTGTCGTTAAG350.1475237091675448No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.14330874604847207No Hit
CATCCCAACAGCCCAGGACCCTGGTCTTCCTGGAGTCCTCACTCCTGCTTTTCCCTCCTTCCGG330.13909378292939936No Hit
CATCCCGGGACTTACGGTGACCTGAACCATCTAGTGTCCGCCACCATGAGTGGGGTAACCACTT310.13066385669125394No Hit
CATCCCGGGGAGGCAGAGATAGGTGGATCCCTGAATTGGGGACGACCTGGTCTATAGGATGAAT310.13066385669125394No Hit
CATCCCGGGAGAAAAAGAAAGAAAAGAACGGTTAAAAAAAGAAGGAAAGCTGTTAACTAAGTCC300.12644889357218123No Hit
CATCCCGGGGCTCTTCTCCCTGACTGATCGGCGCTTGAATTCGCCTGGAGATGTGAGTTACAAC300.12644889357218123No Hit
CATCCCGGGAGTAATATAAATACCTACTTGCAATTAAACACCTCACTCACTAAAGTCTAATTAT290.12223393045310853No Hit
CATCCCGGGGACCTGGCCGGTGCCCTGCGCGGGGCCTCCCCAGGACACACTGACGTAGATTTGC290.12223393045310853No Hit
CATCCCGGGGTTTCGCTGTGAGGCACCTGCCAAGAACATCGCCACCAGCCTGCATGAGATCTGC290.12223393045310853No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC290.12223393045310853No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA280.11801896733403582No Hit
CATCCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGATGAAGTTCG270.11380400421496312No Hit
CATCCCTACAATGGCTAAAACAGCAATGGCCTACAAGGAAAAAATGAAGGAGCTGTCTATGCTG270.11380400421496312No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG270.11380400421496312No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGACCTGGACAA260.1095890410958904No Hit
CATCCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA260.1095890410958904No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC260.1095890410958904No Hit
CATCCCGAGGCGTAGAGATTATTTTTTAGTCAGTAAATCTCCTGATGTAGTACCTGACACACAG250.10537407797681769No Hit
CATCCCGGGATTGCCTGTGTATGAGAAAAACTTGGAAGTGTGCCTGTGTATATAACTCTGTAAA250.10537407797681769No Hit
CATCCCGGGGCCTGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCTGGCC250.10537407797681769No Hit
CATCCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT240.10115911485774498No Hit
CATCCCGGGGGTTCTGGAAATCATGTTCTCCTCACTCATGGTGACAAGCTAAGAAAGCTGAACA240.10115911485774498No Hit
CATCCCGGGGACTGAGGCTGACTAAGTTGTCCCGAGAACTGACTTTCTCCCCGACCCCGTCCTA240.10115911485774498No Hit
CATCCCGGGAAGACCAAGCGTACCATCCAGTTTGTGGACTGGTGCCCCACTGGCTTCAAGGTTG240.10115911485774498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAC150.004583420758.1504350
AGCTAAT150.004583420758.1504340
GAGCTAA150.004583420758.1504339
CGTAGAG150.004583420758.1504339
AGAGCTA150.004583420758.1504338
GCTCGTG150.004583420758.1504358
ATGCCGA150.004583420758.1504349
CGACGGG150.004583420758.1504353
TAGAGCA150.004583420758.1504341
GCGTAGA150.004583420758.1504338
GCCGACG150.004583420758.1504351
CCGACGG150.004583420758.1504352
GCAAGGC351.5195837E-858.1504255
CCTGTCG201.9450515E-458.1504258
CCTCGCC201.9450515E-458.1504248
AACCTCG201.9450515E-458.1504246
TAGCAAG351.5195837E-858.1504247
ACCTCGC201.9450515E-458.1504247
CAAGGCC351.5195837E-858.1504256
CAGAACC201.9450515E-458.1504243
Produced by FastQC (version 0.11.5)