FastQCFastQC Report
Fri 17 Jun 2016
SRR1526804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23660
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA3331.4074387151310228No Hit
CATCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG2641.1158072696534234No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1700.7185122569737954No Hit
CATCCCGGGGAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTCTTGCTCCTCACGCGG1150.48605240912933223No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAAT960.4057480980557904No Hit
CATCCCGGGATCTCAGTTGATTTTCCAAGTAACTAGATGTAGTTGGCCTCCTCTTCTACCACTT820.34657650042265425No Hit
CATCCCAGGAGACTACCTAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT740.3127641589180051No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC690.2916314454775993No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT650.27472527472527475No Hit
CATCCCGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAG610.25781910397295016No Hit
CATCCCGGGACTTACGGTGACCTGAACCATCTAGTGTCCGCCACCATGAGTGGGGTAACCACTT530.22400676246830095No Hit
CATCCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG500.2113271344040575No Hit
CATCCCGGGGGGCACTGTGGGCAGCCCCAGAGGGCCCTGATCCAAGGCACTTATGTAATCAGGA460.19442096365173286No Hit
CATCCCGGGGGGGGCGTAGCAAAGGGCATAAGGCTGTCTGATCATACGGAAAGTCTTAGCGGAC430.18174133558748945No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAGTTCCTGGG420.17751479289940827No Hit
CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA410.17328825021132713No Hit
CATCCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGATGAAGTTCG410.17328825021132713No Hit
CATCCCGGGAGAAGACCGGGTTCGCGTGTGTGCTGTGTCCGTAGTGGCCTTTTCTGGGCCTCGT410.17328825021132713No Hit
CATCCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT390.16483516483516483No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.16483516483516483No Hit
CATCCCGGGGGGCGGGAGAGGAACACTTCTCTCAGGCTTGAGCAGAACCTCGCCCAGCCTGTCG380.16060862214708369No Hit
CATCCCTACAATGGCTAAAACAGCAATGGCCTACAAGGAAAAAATGAAGGAGCTGTCTATGCTG370.15638207945900254No Hit
CATCCCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATGCAAAACAG360.1521555367709214No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG350.14792899408284024No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG340.1437024513947591No Hit
CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTTGGTGTGGC330.13947590870667792No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA330.13947590870667792No Hit
CATCCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG320.1352493660185968No Hit
CATCCCGGGAGTAATATAAATACCTACTTGCAATTAAACACCTCACTCACTAAAGTCTAATTAT320.1352493660185968No Hit
CATCCCGGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTA320.1352493660185968No Hit
CATCCCGGGGGGATCTGAACCATGCTGTGGCTAAGGACCCCGTAAAATAACATCTGTCGTTAAG300.12679628064243448No Hit
CATCCCGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG290.12256973795435333No Hit
CATCCCGGGGCTCTTCTCCCTGACTGATCGGCGCTTGAATTCGCCTGGAGATGTGAGTTACAAC290.12256973795435333No Hit
CATCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC280.1183431952662722No Hit
CATCCCGGGATTGCCTGTGTATGAGAAAAACTTGGAAGTGTGCCTGTGTATATAACTCTGTAAA280.1183431952662722No Hit
CATCCCGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1183431952662722No Hit
CATCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC270.11411665257819104No Hit
CATCCCGGGATGTCATGCATTAGCAGTTTTAAAAAATTAGTTGTTAATTTTTGTTTAAAAGATT260.10989010989010989No Hit
CATCCCCCTCCAGATCTGCTTGTCCTCTGGTCTCAGGTCAAGGGCCCTTTGTGGCCACAGGAGC250.10566356720202875No Hit
CATCCCGGGCCTTCATGGTAGACAATGAGGCCATCTATGACATCTGTCGTAGAAACCTCGACAT250.10566356720202875No Hit
CATCCCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGA240.1014370245139476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGAT150.00449948858.4209445
CCGTTAC150.00449948858.4209444
GATTCTC303.428231E-758.4209443
GCCGTTA150.00449948858.4209443
CGATTCT303.428231E-758.4209442
GCGATTC303.4794903E-758.29637541
TAGTTGG201.9208423E-458.29637540
AGCGATT303.4794903E-758.29637540
GGTGCCG150.004537795758.29637540
AGTCTCG150.00457634658.1723426
CCAAGTA201.9412603E-458.1723425
ATTTTCC201.9412603E-458.1723420
CAAGTAA201.9412603E-458.1723426
GCCTCCT201.9618504E-458.04883646
TGCCGGT201.9618504E-458.04883658
CACGCGG303.5840094E-758.04883658
TCTACCA201.9618504E-458.04883655
TCACGCG303.5840094E-758.04883657
GCAAAGA303.5840094E-758.04883657
AGTCGGG150.00461513958.04883655
Produced by FastQC (version 0.11.5)