FastQCFastQC Report
Fri 17 Jun 2016
SRR1526803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21206
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT3001.4146939545411676No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2010.9478449495425824No Hit
CATCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA1890.8912571913609356No Hit
CATCCCGGGGAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTCTTGCTCC1010.4762802980288598No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA780.36782042818070354No Hit
CATCCCGGGGGGCGGGAGAGGAACACTTCTCTCAGGCTTGAGCAGAACCTCGCCCA770.3631047816655663No Hit
CATCCCAGGAGACTACCTAGATGCTTACACCACATGAAACACTGTCTCTTCTATAG740.3489578421201547No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC680.3206639630293313No Hit
CATCCCGGGGACCTGGCCGGTGCCCTGCGCGGGGCCTCCCCAGGACACACTGACGT590.27822314439309626No Hit
CATCCCGGGATCTCAGTTGATTTTCCAAGTAACTAGATGTAGTTGGCCTCCTCTTC590.27822314439309626No Hit
CATCCCGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGA570.26879185136282185No Hit
CATCCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC490.23106667924172405No Hit
CATCCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG490.23106667924172405No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT430.20277280015090068No Hit
CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTT430.20277280015090068No Hit
CATCCCGGGGGGGGCGTAGCAAAGGGCATAAGGCTGTCTGATCATACGGAAAGTCT410.19334150712062625No Hit
CATCCCGGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCA410.19334150712062625No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAG410.19334150712062625No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC370.17447892106007734No Hit
CATCCCGGGGAGGCAGAGATAGGTGGATCCCTGAATTGGGGACGACCTGGTCTATA370.17447892106007734No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC360.1697632745449401No Hit
CATCCCGGGGGGCACTGTGGGCAGCCCCAGAGGGCCCTGATCCAAGGCACTTATGT340.16033198151466566No Hit
CATCCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGAT330.15561633499952846No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG320.15090068848439123No Hit
CATCCCGGGATTGCCTGTGTATGAGAAAAACTTGGAAGTGTGCCTGTGTATATAAC310.14618504196925397No Hit
CATCCCGGGAAGACCAAGCGTACCATCCAGTTTGTGGACTGGTGCCCCACTGGCTT310.14618504196925397No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC310.14618504196925397No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC300.14146939545411674No Hit
CATCCCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC290.13675374893897954No Hit
CATCCCGGGACTTACGGTGACCTGAACCATCTAGTGTCCGCCACCATGAGTGGGGT290.13675374893897954No Hit
CATCCCGGGAGTAATATAAATACCTACTTGCAATTAAACACCTCACTCACTAAAGT290.13675374893897954No Hit
CATCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC280.1320381024238423No Hit
CATCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT280.1320381024238423No Hit
CATCCCGGGGGGATCTGAACCATGCTGTGGCTAAGGACCCCGTAAAATAACATCTG270.12732245590870508No Hit
CATCCCAACAGCCCAGGACCCTGGTCTTCCTGGAGTCCTCACTCCTGCTTTTCCCT270.12732245590870508No Hit
CATCCCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATG260.12260680939356786No Hit
CATCCCGGGGCTCTTCTCCCTGACTGATCGGCGCTTGAATTCGCCTGGAGATGTGA260.12260680939356786No Hit
CATCCCGGGATGTCATGCATTAGCAGTTTTAAAAAATTAGTTGTTAATTTTTGTTT260.12260680939356786No Hit
CATCCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA260.12260680939356786No Hit
CATCCCGGGAGAAAAAGAAAGAAAAGAACGGTTAAAAAAAGAAGGAAAGCTGTTAA250.11789116287843063No Hit
CATCCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA250.11789116287843063No Hit
CATCCCGGGATCATTAACTTGGAAAGTGCACCCCTCAGGTCCTCCTCTAGAACCCT250.11789116287843063No Hit
CATCCCGGGAGAAGACCGGGTTCGCGTGTGTGCTGTGTCCGTAGTGGCCTTTTCTG240.11317551636329341No Hit
CATCCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC240.11317551636329341No Hit
CATCCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA230.10845986984815618No Hit
CATCCCGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATC230.10845986984815618No Hit
CATCCCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG230.10845986984815618No Hit
CATCCCGGGGGTTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGC220.10374422333301896No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC220.10374422333301896No Hit
CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT220.10374422333301896No Hit
CATCCCGAGGCGTAGAGATTATTTTTTAGTCAGTAAATCTCCTGATGTAGTACCTG220.10374422333301896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAA150.00800613650.48325348
GTGTTGA150.00800613650.48325350
TATAAAT150.00800613650.48325349
AAGATTA251.9057166E-550.48325348
AGAAACA150.00800613650.48325350
CCCTGTA150.00800613650.48325350
AAGAAAC150.00800613650.48325349
GATTAAG251.9057166E-550.48325350
AGATTAA251.9057166E-550.48325349
TGGTAGA204.0407712E-450.12351631
CCGCCAC204.0407712E-450.12351646
GTTCATT204.0407712E-450.12351629
GCTCGTG204.0407712E-450.12351637
GGTAGAG204.0407712E-450.12351632
AGCGCAG204.0407712E-450.12351625
TCATTGG204.0407712E-450.12351631
AACGTTC150.00823650650.12351240
TGTTGCA150.00823650650.12351231
TTGTTGC150.00823650650.12351230
TGCGCGG150.00823650650.12351226
Produced by FastQC (version 0.11.5)