##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526803_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21206 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.96873526360464 40.0 39.0 40.0 38.0 40.0 2 38.02961426011506 39.0 38.0 40.0 35.0 40.0 3 38.703055738941806 39.0 39.0 40.0 36.0 40.0 4 38.8539564274262 39.0 39.0 40.0 38.0 40.0 5 38.950580024521365 40.0 39.0 40.0 38.0 40.0 6 38.923182118268414 39.0 39.0 40.0 38.0 40.0 7 38.90356502876544 39.0 39.0 40.0 38.0 40.0 8 38.921060077336605 39.0 39.0 40.0 38.0 40.0 9 38.89276619824578 39.0 39.0 40.0 38.0 40.0 10 38.12326699990569 39.0 38.0 40.0 35.0 40.0 11 38.155050457417715 39.0 38.0 40.0 35.0 40.0 12 38.00278223144393 39.0 38.0 40.0 35.0 40.0 13 37.76577383759314 39.0 38.0 40.0 34.0 40.0 14 37.941856078468355 39.0 38.0 40.0 34.0 40.0 15 38.02338960671508 39.0 38.0 40.0 35.0 40.0 16 37.874799585023105 39.0 38.0 40.0 35.0 40.0 17 37.82570970480053 39.0 38.0 40.0 34.0 40.0 18 37.713335848344805 39.0 38.0 40.0 33.0 40.0 19 37.65891728756012 39.0 38.0 40.0 33.0 40.0 20 37.5944543996982 39.0 38.0 40.0 33.0 40.0 21 37.4331792888805 39.0 37.0 40.0 33.0 40.0 22 37.348250495142885 39.0 37.0 40.0 33.0 40.0 23 37.248325945487124 39.0 37.0 40.0 33.0 40.0 24 37.087758181646706 39.0 36.0 40.0 33.0 40.0 25 36.97783646137886 39.0 36.0 40.0 33.0 40.0 26 36.53216070923324 39.0 36.0 40.0 32.0 40.0 27 36.294397811940016 38.0 36.0 40.0 31.0 40.0 28 36.16872583231161 38.0 35.0 40.0 31.0 40.0 29 36.069178534377066 38.0 35.0 40.0 30.0 40.0 30 35.71182684145997 38.0 35.0 40.0 30.0 40.0 31 35.04923134961803 38.0 35.0 39.0 28.0 40.0 32 34.72234273318872 38.0 34.0 39.0 27.0 40.0 33 34.58049608601339 38.0 34.0 39.0 27.0 40.0 34 34.77751579741582 38.0 34.0 39.0 27.0 40.0 35 34.481986230312174 38.0 33.0 39.0 27.0 40.0 36 34.24394039422805 38.0 34.0 39.0 25.0 40.0 37 33.88126002074885 38.0 33.0 39.0 23.0 40.0 38 33.61138357068754 37.0 33.0 39.0 22.0 40.0 39 33.611572196548146 37.0 33.0 39.0 23.0 40.0 40 33.245920965764405 37.0 33.0 39.0 19.0 40.0 41 32.560737527114966 36.0 32.0 39.0 11.0 40.0 42 32.44435537112138 36.0 32.0 39.0 10.0 40.0 43 32.34702442704895 36.0 32.0 39.0 4.0 40.0 44 32.00575308874847 36.0 31.0 39.0 4.0 40.0 45 31.61260963878148 36.0 31.0 39.0 4.0 40.0 46 31.064840139583136 36.0 30.0 39.0 4.0 40.0 47 30.795293784777893 36.0 30.0 39.0 4.0 40.0 48 30.50924266716967 35.0 30.0 38.0 4.0 40.0 49 30.095680467792135 35.0 29.0 38.0 4.0 40.0 50 29.665849287937377 35.0 29.0 38.0 4.0 40.0 51 28.69617089502971 34.0 27.0 38.0 4.0 40.0 52 28.443034990097143 34.0 26.0 38.0 4.0 39.0 53 28.129963217957183 34.0 25.0 38.0 4.0 39.0 54 27.583702725643686 33.0 22.0 38.0 4.0 39.0 55 27.298123172686974 33.0 18.0 38.0 4.0 39.0 56 15.819862303121758 19.0 4.0 23.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.1476773594251526 1 2 0.5378851837703138 1 3 -0.20106396376559132 1 4 -0.9172448539484392 1 5 -1.2826421529663037 1 6 -0.7454209557342679 1 7 -0.4228479519211703 1 8 -0.21912715835792085 1 9 -1.350145100294064 1 10 0.69828448782647 1 11 0.16205029349850264 1 12 -0.05173770775567732 1 13 0.10529311896516447 1 14 0.8867964608100962 1 15 0.7970623333083395 1 16 1.2738535595161338 1 17 0.9695674930071476 1 18 0.07817357095037636 1 19 1.088089270940408 1 20 0.07561900401545785 1 21 -1.4262182441589744 1 22 -1.4565736148898054 1 23 -0.7037375007953628 1 24 -2.189075675519092 1 25 -1.0643496881096581 1 26 -1.5574210041172307 1 27 -2.1429323089949364 1 28 -0.8524250057034308 1 29 -0.9147972348277378 1 30 -0.30385757794571333 1 31 -1.063912471611431 1 32 -0.6292521546856591 1 33 -1.9679209299458051 1 34 -2.6595992973675884 1 35 -1.9397142293088834 1 36 -1.3041401257436647 1 37 -0.9834226939890058 1 38 -1.4029939223880028 1 39 -1.9530351852938068 1 40 -1.3246038924132186 1 41 -0.5675169097711219 1 42 -0.36951525340121805 1 43 -0.14052081548373963 1 44 -0.11684119757006428 1 45 -0.10237491617054317 1 46 -0.6064238966061986 1 47 0.9472196534116009 1 48 -0.8509599722025314 1 49 0.18177612670419308 1 50 -0.5668202187815474 1 51 0.5912670696241982 1 52 1.4637918186608267 1 53 0.7556436236477566 1 54 1.7387373910066906 1 55 2.0590639437268337 1 56 2.015382234211753 2 1 -0.16548053531168705 2 2 0.5668325521913715 2 3 -0.07211659534453929 2 4 0.024860409209459533 2 5 0.01472626808632782 2 6 -0.053315692576369145 2 7 0.17978362702619677 2 8 -0.3954429478316044 2 9 0.15248647865330867 2 10 0.15881080361594258 2 11 -0.927423390712022 2 12 -1.7938429709135733 2 13 0.5710825926493754 2 14 -0.38951932866358874 2 15 0.04969391225571229 2 16 0.43701145425297483 2 17 -0.3567482964665345 2 18 -0.8297211658917263 2 19 0.4433524288351478 2 20 -0.20595994335295842 2 21 -0.4788498231063443 2 22 0.2750053324786137 2 23 0.6909993413098974 2 24 0.013555903428276395 2 25 0.6145976803113982 2 26 1.0636316274617172 2 27 1.3307519015313787 2 28 0.04757499429656775 2 29 -0.9674288137751077 2 30 0.8066687378437578 2 31 -0.9191756295061708 2 32 0.47074784531434233 2 33 0.6873422279489318 2 34 0.2325059657903097 2 35 0.7050226127963768 2 36 1.2563861900458093 2 37 1.103419411274153 2 38 1.2259534460330528 2 39 1.0601227094430357 2 40 1.4490803181130971 2 41 1.6877462481236165 2 42 1.1515373781777285 2 43 -0.0826260786416384 2 44 3.341053539272039 2 45 1.9713092943557733 2 46 0.785681366551696 2 47 -1.281727715009449 2 48 -1.8746441827288471 2 49 0.08440770565156441 2 50 -2.0431360082552317 2 51 0.06495128015051321 2 52 -1.1309450234444363 2 53 0.5819594131214423 2 54 0.4308426541645858 2 55 1.069590259516307 2 56 1.2153822342117522 3 1 0.7292563067935731 3 2 0.9878851837703166 3 3 0.7173570888659881 3 4 0.7090709355252471 3 5 0.6989367944021154 3 6 0.7361579916341512 3 7 0.49557310071040916 3 8 0.7624517890104983 3 9 0.2577496365480414 3 10 0.6324950141422576 3 11 0.019945030340608128 3 12 0.627209660665379 3 13 0.20266154001779313 3 14 0.29469119765219887 3 15 0.31285180699255477 3 16 0.43701145425297483 3 17 0.5906201245860956 3 18 0.6965946235819587 3 19 1.1275629551509354 3 20 1.2150926882259867 3 21 1.5211501768936557 3 22 1.643426385110196 3 23 1.6383677623625346 3 24 1.645134850796694 3 25 1.2988082066271858 3 26 1.5899474169354022 3 27 1.5412782173208512 3 28 2.41599604692815 3 29 1.9273080283301596 3 30 3.227721369422703 3 31 1.238719107335939 3 32 2.681274161103815 3 33 -0.10213145626159559 3 34 0.7061901763166247 3 35 -1.0844510714141435 3 36 1.940596716361597 3 37 1.9981562533794133 3 38 2.068058709190943 3 39 2.323280604179878 3 40 1.6596066339025697 3 41 3.5298515112815068 3 42 1.6778531676514135 3 43 3.233163395042574 3 44 2.814737749798354 3 45 3.2870987680399857 3 46 2.6277866297095898 3 47 2.7182722849905474 3 48 3.96746108042905 3 49 3.189670863546297 3 50 2.588442939113193 3 51 3.064951280150513 3 52 2.3953707660292487 3 53 3.2135383604898635 3 54 3.588737391006692 3 55 4.332748154253149 3 56 -0.4688282921040372 4 1 -0.04517978343199047 4 2 -0.8692576733725375 4 3 -0.6510639637655942 4 4 -0.7696258063293868 4 5 -0.12311834344249206 4 6 -0.0382780985914124 4 7 -0.01570509477831905 4 8 0.22611093688017547 4 9 0.19509299494403365 4 10 -0.09933455979258099 4 11 -0.13794970650149452 4 12 -0.0945948506128218 4 13 0.30291216658421405 4 14 -0.006060682047049681 4 15 0.3303956666416781 4 16 -0.44519405953148805 4 17 -0.49709917365951384 4 18 0.04245928523609166 4 19 -0.13810120525006653 4 20 -0.24104766265120503 4 21 1.288067470126741 4 22 0.5481882898721011 4 23 -0.3465946436525087 4 24 0.7704481340047167 4 25 1.0832693595093943 4 26 1.833055186358962 4 27 0.9523057862431585 4 28 0.5237654704870423 4 29 1.7042503842198826 4 30 0.9770948030066648 4 31 2.7575160998171384 4 32 2.3755097500762474 4 33 0.7249362129113379 4 34 2.0094483216800327 4 35 2.0960000564054013 4 36 4.2839551123515776 4 37 3.3665773060109956 4 38 2.2660536966596183 4 39 2.2731552908966677 4 40 3.118253250443928 4 41 3.6301021378479277 4 42 2.254294270408309 4 43 1.0928125178495947 4 44 2.0403016595727905 4 45 1.735720321924699 4 46 -0.05642389660619784 4 47 0.10674346293541248 4 48 0.8371352658927051 4 49 -0.2539381590100902 4 50 0.7212750193136905 4 51 3.2103146886718186 4 52 2.71617277104178 4 53 0.9604055284096624 4 54 1.160165962435265 4 55 2.716206800869692 4 56 1.0249060437355624 5 1 0.2087884705362697 5 2 -0.12322592734079763 5 3 0.015602702901070131 5 4 0.46053292382934075 5 5 0.3451356248114763 5 6 0.32680126648795493 5 7 0.4049298258566054 5 8 0.5197617305309663 5 9 0.5998548997059387 5 10 1.44828448782647 5 11 -0.02683859539038025 5 12 0.49270673668876697 5 13 0.8664042300762773 5 14 0.9145742385878748 5 15 -0.16960433335832192 5 16 0.7294091150716895 5 17 1.2251230485627076 5 18 0.2726180153948192 5 19 0.8936448264959651 5 20 0.4811745595710164 5 21 1.3515595336188042 5 22 0.4212041628879746 5 23 0.5184847214268586 5 24 1.1275909911475708 5 25 -0.24212746588743528 5 26 2.3648012181049936 5 27 1.468178802116178 5 28 1.7697972165187892 5 29 1.974091654061148 5 30 3.429475755387621 5 31 2.5749764172774547 5 32 1.859636734203228 5 33 3.637634625609749 5 34 2.5015118137435266 5 35 2.6436191040244523 5 36 3.101415429811894 5 37 1.9221328615665527 5 38 3.147006077611998 5 39 3.4636314813728575 5 40 3.5865072186978963 5 41 3.638038645784434 5 42 2.508262524376562 5 43 3.0928125178495947 5 44 2.460936580207715 5 45 2.4976250838294582 5 46 2.388020547838245 5 47 2.186108542300488 5 48 2.099040027797468 5 49 3.6428872378153088 5 50 2.0387353367740104 5 51 5.369044847401977 5 52 4.7637918186608275 5 53 4.833421401425536 5 54 5.422070724340028 5 55 6.636841721504613 5 56 3.88204890087842 6 1 0.33577259752039623 6 2 -0.25021005432492416 6 3 0.39655508385345684 6 4 0.420850384146803 6 5 0.40069118036703344 6 6 0.3902933299800182 6 7 0.36524728617406765 6 8 0.41658712735636527 6 9 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1.579289913227285 114 28 2.269797216518789 114 29 0.1963138762833765 114 30 -0.570524244612379 114 31 1.4083097506107904 114 32 0.4151922897587852 114 33 1.0265235144986349 114 34 1.8348451470768552 114 35 1.1436191040244523 114 36 1.9347487631452225 114 37 2.3110217504554385 114 38 2.647006077611998 114 39 1.9080759258173074 114 40 2.475396107586782 114 41 4.138038645784434 114 42 2.452706968821005 114 43 3.1483680734051447 114 44 4.349825469096601 114 45 3.886513972718344 114 46 5.054687214504916 114 47 4.686108542300488 114 48 4.765706694464132 114 49 2.0873316822597516 114 50 1.4276242256628962 114 51 4.091267069624198 114 52 3.9304584853274918 114 53 4.0000880680922 114 54 4.588737391006692 114 55 4.636841721504613 114 56 2.826493345322863 115 1 0.47862974037753503 115 2 1.0355042313893676 115 3 0.49179317909155174 115 4 0.23037419367061318 115 5 0.25783403750988754 115 6 0.4379123775990692 115 7 0.5081044290312065 115 8 0.3689680797373214 115 9 0.48080728065831835 115 10 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115 48 0.4085638373212781 115 49 0.46034755527562154 115 50 1.1498464478851211 115 51 2.353171831528961 115 52 0.09712515199415961 115 53 1.2461198141239471 115 54 1.0649278671971665 115 55 1.0019210865839767 115 56 -2.451284432454914 116 1 -0.06898930724150887 116 2 -0.2343370384519048 116 3 -0.26217507487670844 116 4 0.07164403494044791 116 5 0.011802291478140603 116 6 0.21569015537684777 116 7 0.12715204807882685 116 8 0.24198395275319484 116 9 0.09985489970593875 116 10 0.6149511544931343 116 11 0.3620502934985055 116 12 0.0482622922443241 116 13 -0.5780402143681727 116 14 -0.3632035391899038 116 15 0.05261788886389951 116 16 -0.992813107150532 116 17 -0.552654729215071 116 18 0.2170624598392621 116 19 -1.3285773957262563 116 20 -0.6299365515400979 116 21 -1.75955157749231 116 22 -1.4676847260009183 116 23 -1.7037375007953628 116 24 -0.26129789774131496 116 25 0.31342808966812186 116 26 -1.2463098930061136 116 27 -1.476265642328272 116 28 -1.1190916723700965 116 29 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49 2.189670863546297 117 50 1.4305482022710834 117 51 0.012319701203146849 117 52 -0.13094502344443626 117 53 0.8451173078582848 117 54 1.588737391006692 117 55 -1.7725150036415869 117 56 0.42590855000122474 118 1 0.20294051731989526 118 2 -0.22264113201915592 118 3 0.243672878339666 118 4 0.3406498828936648 118 5 0.3305157417705402 118 6 0.26247378110783615 118 7 0.39030994281566933 118 8 0.39403073637892305 118 9 0.36301279444278123 118 10 0.8430213299317302 118 11 0.5462608198142931 118 12 0.7851043975074816 118 13 0.9921352242283206 118 14 -0.07372985497937634 118 15 0.26022022804518485 118 16 0.06859040162139252 118 17 -0.09359040172969202 118 18 0.7492262025293215 118 19 -0.13559493958590707 118 20 -0.5217494170371708 118 21 0.3632554400515531 118 22 0.38026849037335353 118 23 0.8488940781520071 118 24 0.5398716929019614 118 25 -1.1222444249517594 118 26 1.1162632064090872 118 27 1.0675940067945362 118 28 0.2054697311386704 118 29 0.2957290809617348 118 30 0.6487740010016552 118 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-2.471365673327 119 51 -3.0905511121939853 119 52 -3.8725718177028092 119 53 -2.717083649079516 119 54 -2.7294444271751246 119 55 -2.3682087835458923 119 56 -1.9209814021518845 120 1 0.5187299910041006 120 2 -1.3805358688612657 120 3 0.7173570888659881 120 4 0.6038077776305073 120 5 0.48841047861264286 120 6 0.4730000968973158 120 7 0.17978362702619677 120 8 0.02560968374734074 120 9 0.20511805760067858 120 10 0.15881080361594258 120 11 1.072576609287978 120 12 0.8903675554022215 120 13 0.20266154001779313 120 14 0.5052175134416785 120 15 1.154957070150445 120 16 1.0159588226740297 120 17 0.6958832824808354 120 18 1.2755419920030064 120 19 1.2328261130456681 120 20 1.1624611092786168 120 21 1.8369396505778681 120 22 1.2223737535312509 120 23 1.2699467097309523 120 24 1.3819769560598516 120 25 1.2461766276798159 120 26 1.9583684695669845 120 27 1.3833834804787486 120 28 2.468627625875513 120 29 1.8220448704354197 120 30 2.12245821152797 120 31 3.659771738914884 120 32 3.3128531084722397 120 33 2.7399738068962947 120 34 2.653558597369255 120 35 3.8102857706911166 120 36 3.835333558466864 120 37 4.208682569168893 120 38 4.173321867085683 120 39 4.6390700778640905 120 40 3.8701329496920422 120 41 5.003535721807822 120 42 4.730484746598783 120 43 5.180531816095204 120 44 5.077895644535197 120 45 4.392361925934718 120 46 4.048839261288542 120 47 6.03406175867476 120 48 6.230618975165886 120 49 5.715986653019982 120 50 5.483179781218453 120 51 5.170214438045253 120 52 6.7111602397134575 120 53 5.687222571016175 120 54 6.483474233111952 120 55 7.122221838463677 120 56 0.8469611815801734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 1.0 6 0.0 7 4.0 8 6.0 9 2.0 10 10.0 11 11.0 12 15.0 13 23.0 14 45.0 15 54.0 16 72.0 17 98.0 18 116.0 19 123.0 20 129.0 21 200.0 22 201.0 23 199.0 24 251.0 25 319.0 26 338.0 27 344.0 28 402.0 29 421.0 30 486.0 31 561.0 32 678.0 33 904.0 34 1146.0 35 1635.0 36 2442.0 37 3513.0 38 4194.0 39 2258.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 97.06686786758465 1.7919456757521455 0.44798641893803637 0.6932000377251721 8 97.90536396659904 1.0850592064914848 0.4293060338727179 0.5802707930367506 9 96.05771951334528 1.570310289540696 1.1600490427237575 1.2119211543902668 10 50.42440818636235 39.07856267094218 4.659058756955579 5.837970385739885 11 34.32519098368386 24.653399981137415 27.487503536734888 13.533905498443838 12 42.0447043289635 13.826275582382344 21.663680090540414 22.46533999811374 13 28.96350089597284 18.81071394888239 24.941054418560785 27.284730736583985 14 25.46920682825615 19.654814675091956 30.015090068848437 24.860888427803452 15 25.846458549467133 25.096670753560314 24.945770065075923 24.111100631896633 16 32.627558238234464 23.634820333867772 22.295576723568804 21.442044704328964 17 30.609261529755727 20.517777987362066 25.091955107045177 23.781005375837026 18 30.500801659907573 22.65868150523437 23.851740073564084 22.988776761293973 19 31.92492690747902 22.319154956144487 21.23927190417806 24.516646232198436 20 29.28416485900217 23.40375365462605 23.45562576629256 23.85645572007922 21 30.36876355748373 21.399603885692727 26.035084410072624 22.19654814675092 22 28.793737621427894 20.68282561539187 24.224276148259925 26.299160614920307 23 25.23342450249929 24.818447609167215 24.29972649250212 25.648401395831367 24 26.27682150436218 25.564725300636642 23.249233671303937 24.90921952369724 25 27.067314495966794 25.54365771970376 22.137836690409927 25.251191093919523 26 25.94497664102685 24.265018168090226 21.891368977396063 27.89863621348686 27 24.06454961543906 21.592035105931203 25.371584957297223 28.971830321332515 28 25.571077968661506 24.08910704172173 23.971115725882576 26.36869926373419 29 28.066115702479337 23.09327036599764 21.497048406139317 27.343565525383706 30 27.657764121956983 20.883951784448122 25.015362798392815 26.442921295202083 31 26.35787282361847 24.43224829674489 22.885124905374717 26.32475397426192 32 28.69306087696892 24.23726408400738 24.880563833309683 22.189111205714017 33 30.407419675389203 21.487720626508306 23.53664884304169 24.568210855060805 34 26.60797955416726 25.48156562071087 25.060343603578023 22.85011122154385 35 26.041666666666668 24.44128787878788 26.581439393939394 22.935606060606062 36 29.422074846044527 24.91710090004737 22.695405021316912 22.965419232591188 37 27.5893110963707 24.09741305789823 24.594901923623613 23.718373922107457 38 28.436715158982135 25.219163152158465 25.479789603373927 20.864332085485476 39 28.728548402389304 22.854840238930503 21.87825922063146 26.538352138048733 40 26.906467992217532 21.43026621743463 23.926351255160633 27.736914535187207 41 26.36924537256763 23.502610346464166 26.64451827242525 23.483626008542952 42 25.735712929561423 21.33092842225176 23.860831592937156 29.07252705524967 43 25.51524361287872 20.486275999620098 27.98461392344952 26.013866464051667 44 25.800170956406117 21.89191756102194 27.006363377338776 25.301548105233167 45 25.28140584184279 20.97838993113275 27.679886012823555 26.0603182142009 46 25.21734999287377 22.010546819326336 23.811107416029266 28.96099577177063 47 25.402861624756383 23.7296192422874 25.250748680895562 25.616770452060656 48 26.765587102297044 23.04655918580872 25.00594473771817 25.18190897417606 49 27.700646633701027 23.95397489539749 23.430962343096233 24.91441612780525 50 27.00028541527923 22.495480924745504 24.05099419655599 26.453239463419276 51 23.60159817351598 24.97146118721461 25.84189497716895 25.58504566210046 52 27.415441701155984 23.643023643023643 24.80852480852481 24.13300984729556 53 27.223860717343733 21.972219579488154 25.558938255161262 25.244981448006847 54 25.09467427256603 23.694933128804944 25.72264033363693 25.487752264992093 55 27.558792725887844 22.5078548986004 24.754831952775397 25.178520422736362 56 25.14397220503546 23.954119270858122 24.625196325734137 26.27671219837228 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 2.0 11 3.0 12 6.0 13 10.5 14 12.0 15 31.0 16 50.0 17 50.0 18 50.0 19 43.5 20 37.0 21 40.0 22 39.5 23 36.0 24 60.5 25 85.0 26 91.5 27 98.0 28 151.5 29 228.5 30 252.0 31 223.0 32 194.0 33 208.0 34 222.0 35 255.5 36 289.0 37 298.0 38 310.0 39 313.0 40 353.5 41 394.0 42 386.0 43 378.0 44 444.0 45 510.0 46 581.0 47 825.5 48 999.0 49 1020.5 50 1042.0 51 917.0 52 792.0 53 872.5 54 1025.5 55 1098.0 56 1118.0 57 1138.0 58 1250.0 59 1362.0 60 1443.0 61 1524.0 62 1600.0 63 1636.0 64 1596.0 65 1432.5 66 1269.0 67 1112.5 68 956.0 69 834.5 70 713.0 71 751.0 72 587.5 73 386.0 74 366.5 75 347.0 76 272.5 77 198.0 78 149.0 79 83.0 80 66.0 81 49.5 82 33.0 83 23.0 84 13.0 85 11.0 86 9.0 87 7.0 88 2.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.04244081863623503 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004715646515137226 25 0.03300952560596058 26 0.07073469772705837 27 0.06130340469678393 28 0.08488163727247006 29 0.14618504196925397 30 0.24049797227199848 31 0.33009525605960577 32 0.3065170234839197 33 0.34424219560501745 34 0.3631047816655663 35 0.40554560030180137 36 0.45270206545317365 37 0.4715646515137225 38 0.48571159105913425 39 0.5281524096953693 40 0.6271809865132509 41 0.6413279260586626 42 0.6507592190889371 43 0.6979156842403094 44 0.6979156842403094 45 0.712062623785721 46 0.7403565028765444 47 0.7969442610581912 48 0.8441007262095633 49 0.8205224936338773 50 0.8676789587852495 51 0.8582476657549749 52 0.8723946053003867 53 0.8676789587852495 54 1.6268980477223427 55 0.943129303027445 56 0.9195510704517589 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 21206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.079883051966426 #Duplication Level Percentage of deduplicated Percentage of total 1 70.35635155096011 35.93794209186079 2 14.281757754800593 14.590210317834575 3 6.370014771048744 9.761388286334057 4 3.305022156573117 6.752805809676507 5 1.4586410635155096 3.7253607469584082 6 1.006277695716396 3.0840328208997456 7 0.6185376661742984 2.2116382155993586 8 0.45236336779911374 1.8485334339337924 9 0.3877400295420975 1.7825143827218712 >10 1.6617429837518465 14.349712345562576 >50 0.06462333825701624 2.22578515514477 >100 0.03692762186115214 3.730076393473545 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 300 1.4146939545411676 No Hit CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 201 0.9478449495425824 No Hit CATCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 189 0.8912571913609356 No Hit CATCCCGGGGAGCAAAGTTCGTTTTCCTGGGTAGGGAAGAGCGATTCTCTTGCTCC 101 0.4762802980288598 No Hit CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA 78 0.36782042818070354 No Hit CATCCCGGGGGGCGGGAGAGGAACACTTCTCTCAGGCTTGAGCAGAACCTCGCCCA 77 0.3631047816655663 No Hit CATCCCAGGAGACTACCTAGATGCTTACACCACATGAAACACTGTCTCTTCTATAG 74 0.3489578421201547 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 68 0.3206639630293313 No Hit CATCCCGGGGACCTGGCCGGTGCCCTGCGCGGGGCCTCCCCAGGACACACTGACGT 59 0.27822314439309626 No Hit CATCCCGGGATCTCAGTTGATTTTCCAAGTAACTAGATGTAGTTGGCCTCCTCTTC 59 0.27822314439309626 No Hit CATCCCGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGA 57 0.26879185136282185 No Hit CATCCCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 49 0.23106667924172405 No Hit CATCCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 49 0.23106667924172405 No Hit CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 43 0.20277280015090068 No Hit CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTT 43 0.20277280015090068 No Hit CATCCCGGGGGGGGCGTAGCAAAGGGCATAAGGCTGTCTGATCATACGGAAAGTCT 41 0.19334150712062625 No Hit CATCCCGGGGGGCCCACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCA 41 0.19334150712062625 No Hit CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAG 41 0.19334150712062625 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 37 0.17447892106007734 No Hit CATCCCGGGGAGGCAGAGATAGGTGGATCCCTGAATTGGGGACGACCTGGTCTATA 37 0.17447892106007734 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 36 0.1697632745449401 No Hit CATCCCGGGGGGCACTGTGGGCAGCCCCAGAGGGCCCTGATCCAAGGCACTTATGT 34 0.16033198151466566 No Hit CATCCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGAT 33 0.15561633499952846 No Hit CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 32 0.15090068848439123 No Hit CATCCCGGGATTGCCTGTGTATGAGAAAAACTTGGAAGTGTGCCTGTGTATATAAC 31 0.14618504196925397 No Hit CATCCCGGGAAGACCAAGCGTACCATCCAGTTTGTGGACTGGTGCCCCACTGGCTT 31 0.14618504196925397 No Hit CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 31 0.14618504196925397 No Hit CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 30 0.14146939545411674 No Hit CATCCCGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC 29 0.13675374893897954 No Hit CATCCCGGGACTTACGGTGACCTGAACCATCTAGTGTCCGCCACCATGAGTGGGGT 29 0.13675374893897954 No Hit CATCCCGGGAGTAATATAAATACCTACTTGCAATTAAACACCTCACTCACTAAAGT 29 0.13675374893897954 No Hit CATCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 28 0.1320381024238423 No Hit CATCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 28 0.1320381024238423 No Hit CATCCCGGGGGGATCTGAACCATGCTGTGGCTAAGGACCCCGTAAAATAACATCTG 27 0.12732245590870508 No Hit CATCCCAACAGCCCAGGACCCTGGTCTTCCTGGAGTCCTCACTCCTGCTTTTCCCT 27 0.12732245590870508 No Hit CATCCCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATG 26 0.12260680939356786 No Hit CATCCCGGGGCTCTTCTCCCTGACTGATCGGCGCTTGAATTCGCCTGGAGATGTGA 26 0.12260680939356786 No Hit CATCCCGGGATGTCATGCATTAGCAGTTTTAAAAAATTAGTTGTTAATTTTTGTTT 26 0.12260680939356786 No Hit CATCCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 26 0.12260680939356786 No Hit CATCCCGGGAGAAAAAGAAAGAAAAGAACGGTTAAAAAAAGAAGGAAAGCTGTTAA 25 0.11789116287843063 No Hit CATCCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 25 0.11789116287843063 No Hit CATCCCGGGATCATTAACTTGGAAAGTGCACCCCTCAGGTCCTCCTCTAGAACCCT 25 0.11789116287843063 No Hit CATCCCGGGAGAAGACCGGGTTCGCGTGTGTGCTGTGTCCGTAGTGGCCTTTTCTG 24 0.11317551636329341 No Hit CATCCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 24 0.11317551636329341 No Hit CATCCCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 23 0.10845986984815618 No Hit CATCCCGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATC 23 0.10845986984815618 No Hit CATCCCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 23 0.10845986984815618 No Hit CATCCCGGGGGTTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGC 22 0.10374422333301896 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 22 0.10374422333301896 No Hit CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT 22 0.10374422333301896 No Hit CATCCCGAGGCGTAGAGATTATTTTTTAGTCAGTAAATCTCCTGATGTAGTACCTG 22 0.10374422333301896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.04244081863623503 0.0 0.0 0.0 0.0 14 0.04244081863623503 0.0 0.0 0.0 0.0 15 0.04244081863623503 0.0 0.0 0.0 0.0 16 0.04715646515137226 0.0 0.0 0.0 0.0 17 0.04715646515137226 0.0 0.0 0.0 0.0 18 0.04715646515137226 0.0 0.0 0.0 0.0 19 0.04715646515137226 0.0 0.0 0.0 0.0 20 0.056587758181646707 0.0 0.0 0.0 0.0 21 0.06601905121192116 0.0 0.0 0.0 0.0 22 0.06601905121192116 0.0 0.0 0.0 0.0 23 0.06601905121192116 0.0 0.0 0.0 0.0 24 0.06601905121192116 0.0 0.0 0.0 0.0 25 0.07073469772705838 0.0 0.0 0.0 0.0 26 0.07073469772705838 0.0 0.0 0.0 0.0 27 0.07073469772705838 0.0 0.0 0.0 0.0 28 0.07073469772705838 0.0 0.0 0.0 0.0 29 0.07073469772705838 0.0 0.0 0.0 0.0 30 0.0754503442421956 0.0 0.0 0.0 0.0 31 0.08016599075733283 0.0 0.0 0.0 0.0 32 0.08959728378760728 0.0 0.0 0.0 0.0 33 0.09431293030274451 0.0 0.0 0.0 0.0 34 0.09431293030274451 0.0 0.0 0.0 0.0 35 0.11317551636329341 0.0 0.0 0.0 0.0 36 0.12260680939356786 0.0 0.0 0.0 0.0 37 0.14146939545411677 0.0 0.0 0.0 0.0 38 0.1509006884843912 0.0 0.0 0.0 0.0 39 0.16033198151466566 0.0 0.0 0.0 0.0 40 0.17447892106007734 0.0 0.0 0.0 0.0 41 0.17447892106007734 0.0 0.0 0.0 0.0 42 0.17447892106007734 0.0 0.0 0.0 0.0 43 0.17447892106007734 0.0 0.0 0.0 0.0 44 0.17919456757521457 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGAAA 15 0.008006136 50.483253 48 GTGTTGA 15 0.008006136 50.483253 50 TATAAAT 15 0.008006136 50.483253 49 AAGATTA 25 1.9057166E-5 50.483253 48 AGAAACA 15 0.008006136 50.483253 50 CCCTGTA 15 0.008006136 50.483253 50 AAGAAAC 15 0.008006136 50.483253 49 GATTAAG 25 1.9057166E-5 50.483253 50 AGATTAA 25 1.9057166E-5 50.483253 49 TGGTAGA 20 4.0407712E-4 50.123516 31 CCGCCAC 20 4.0407712E-4 50.123516 46 GTTCATT 20 4.0407712E-4 50.123516 29 GCTCGTG 20 4.0407712E-4 50.123516 37 GGTAGAG 20 4.0407712E-4 50.123516 32 AGCGCAG 20 4.0407712E-4 50.123516 25 TCATTGG 20 4.0407712E-4 50.123516 31 AACGTTC 15 0.008236506 50.123512 40 TGTTGCA 15 0.008236506 50.123512 31 TTGTTGC 15 0.008236506 50.123512 30 TGCGCGG 15 0.008236506 50.123512 26 >>END_MODULE