FastQCFastQC Report
Fri 17 Jun 2016
SRR1526802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23755
Sequences flagged as poor quality0
Sequence length64
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3221.3555041043990739No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2701.1366028204588507No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2581.0860871395495686No Hit
ACCTGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.4251736476531256No Hit
ACCTGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.3367712060618817No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.3199326457587876No Hit
ACCTGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.23573984424331718No Hit
ACCTGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.17259524310671437No Hit
ACCTGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.16417596295516734No Hit
ACCTGAGGGCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATCA380.1599663228793938No Hit
ACCTGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.1599663228793938No Hit
ACCTGAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.15154704272784678No Hit
ACCTGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.10945064197011155No Hit
ACCTGAGGGGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGG240.10103136181856451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAATT150.00458540658.14406646
TATCACC201.9873807E-457.8987416
CCATATT150.004663056757.89873525
TATTAGC150.004663056757.89873528
CATATTA150.004663056757.89873526
ATATTAG150.004663056757.89873527
AGCTGGT150.004663056757.89873511
AGGGGTA501.8189894E-1257.7768486
GAAGTGT202.0082796E-457.7768449
GAAGTGG453.092282E-1157.7768449
AGGGTTC202.0082796E-457.7768446
AGGGTGG800.057.7768446
AGGGATA202.0082796E-457.7768446
AAGATGT202.0082796E-457.77684410
GGGAATG202.0082796E-457.7768447
GGGAACG202.0082796E-457.7768447
GAGGGTT406.9303496E-1057.7768445
GGGTGGC202.0082796E-457.7768447
GGAGCAG202.0082796E-457.7768448
GGGCCTA202.0082796E-457.7768447
Produced by FastQC (version 0.11.5)