FastQCFastQC Report
Fri 17 Jun 2016
SRR1526801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27284
Sequences flagged as poor quality0
Sequence length64
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5441.9938425450813664No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3541.297463714997801No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.8393197478375605No Hit
ACCTGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.4251576015247031No Hit
ACCTGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.4031666911010116No Hit
ACCTGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG970.35551971851634656No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.3115378976689635No Hit
ACCTGAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG690.25289546987245276No Hit
ACCTGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.2492303181351708No Hit
ACCTGAGGGGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGG580.21257880076235156No Hit
ACCTGAGGGCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATCA530.19425304207594196No Hit
ACCTGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.18325758686409618No Hit
ACCTGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.17592728338953234No Hit
ACCTGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.16493182817768656No Hit
ACCTGAGGGAAGTGGCTTTAATGACCCTGAAGGAATTTCTTCTGGTGATAGCTTTTATATTATC440.16126667644040463No Hit
ACCTGAGGGAGGGGCGGCAGAGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.15393637296584078No Hit
ACCTGAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.15027122122855888No Hit
ACCTGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG400.14660606949127694No Hit
ACCTGAGGGGCCATCTATGACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACCA360.13194546254214926No Hit
ACCTGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG340.1246151590675854No Hit
ACCTGAGGGGTTTAATTTTGCATGTGATTTCTTTTTTCGTGCATCAATGAGGTTTAGTGACATT310.11361970385573962No Hit
ACCTGAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1099545521184577No Hit
ACCTGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1099545521184577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGG201.952565E-458.13653653
GTTTGAT201.952565E-458.13653648
CAGTACG201.952565E-458.13653637
TGATCCT201.952565E-458.13653651
CTGGCTC201.952565E-458.13653656
AGTACGT201.952565E-458.13653638
TCAGTAC201.952565E-458.13653636
TACGTAG201.952565E-458.13653640
GATCCTG201.952565E-458.13653652
CTCAGTA201.952565E-458.13653635
TTGATCC201.952565E-458.13653650
TAGAGTT201.952565E-458.13653644
AGTTTGA201.952565E-458.13653647
GTACGTA201.952565E-458.13653639
CCTGGCT201.952565E-458.13653655
TTTGATC201.952565E-458.13653649
TGCATCA150.00459512658.1365340
CAATGAG150.00459512658.1365345
GTGCATC150.00459512658.1365339
TTCGTGC150.00459512658.1365336
Produced by FastQC (version 0.11.5)