FastQCFastQC Report
Fri 17 Jun 2016
SRR1526800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26873
Sequences flagged as poor quality0
Sequence length64
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4821.7936218509284414No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3261.2131135340304395No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.9340229970602463No Hit
ACCTGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.4279388233542961No Hit
ACCTGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG800.29769657276820605No Hit
ACCTGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.27536932981059054No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.26048450117218025No Hit
ACCTGAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG700.26048450117218025No Hit
ACCTGAGGGCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCATCA670.24932087969337255No Hit
ACCTGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.2046663937781416No Hit
ACCTGAGGGAAGTGGCTTTAATGACCCTGAAGGAATTTCTTCTGGTGATAGCTTTTATATTATC500.18606035798012874No Hit
ACCTGAGGGGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGG450.16745432218211587No Hit
ACCTGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG400.14884828638410302No Hit
ACCTGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.14512707922450044No Hit
ACCTGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1339634577456927No Hit
ACCTGAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG350.13024225058609012No Hit
ACCTGAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.1190786291072824No Hit
ACCTGAGGGAGGGGCGGCAGAGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.11535742194767984No Hit
ACCTGAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11163621478807725No Hit
ACCTGAGGGGCCATCTATGACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACCA290.10791500762847468No Hit
ACCTGAGGGGTTTAATTTTGCATGTGATTTCTTTTTTCGTGCATCAATGAGGTTTAGTGACATT290.10791500762847468No Hit
ACCTGAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.10419380046887211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAA150.00455013658.278222
AGAGAAC150.004584187658.16885421
CTGCCGA150.004584187658.16885421
AGGGGCA550.058.059936
GGCATGG550.058.059938
AGGGGCT406.693881E-1058.0599256
GGATGGA150.00461842858.0599258
AGGGGAC150.00461842858.0599256
GGACTGT150.00461842858.0599258
AGGGTTA150.00461842858.0599256
AGGGTAC303.597761E-758.0599256
GGGAGTT150.00461842858.0599257
AGGGAGT1450.058.0599256
AGGGACG351.5486876E-858.0599256
AGGGACA800.058.0599256
TGAGGTG150.00461842858.0599254
GGGACAT351.5486876E-858.0599257
GGGACAG406.693881E-1058.0599257
GGAGGGT201.9648028E-458.0599258
GGGGCAG406.693881E-1058.0599257
Produced by FastQC (version 0.11.5)